We narrowed to 46,018 results for: cha
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Plasmid#207991PurposeCRISPR vector used with pAf-CRISPR-ctfR1 (#207992) to delete both aflatoxin and cyclopiazonic acid gene clusters of Aspergillus flavusDepositorInsertphoA
UseCRISPRPromoterAspergillus flavus U6Available SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAVA-LGF2-Gal11p
Plasmid#127481PurposePositive control for the AVA-seq system. LGF2 (lambda CI associated) and Gal11p (RNAp associated) interactionDepositorInsertLGF2-Gal11p (GAL11 Budding Yeast)
ExpressionBacterialAvailable SinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSBL_DK202
Plasmid#156426PurposeMammalian expression of 3a delta N(aa 42-275)-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cellsDepositorInsertSARS-CoV-2 3a protein (aa 42-275)
TagsEGFP-HisExpressionMammalianMutationaa 42-275PromoterCMVAvailable SinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGS1022
Plasmid#59576PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a URA3 selection markerDepositorInsertTwin-Strep tag optimized for PCR amplification followed by URA3 selection marker
UseE. coli cloning vectorTagsTwin-Strep tagPromoterNoneAvailable SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAVA-Gal11p-LGF2
Plasmid#127480PurposePositive control for the AVA-seq system. Gal11p (lambda CI associated) and LGF2 (RNAp associated) interactionDepositorInsertGal11p-LGF2 (GAL11 Budding Yeast)
ExpressionBacterialAvailable SinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA0055
Plasmid#131774PurposeGuide RNA (gCASS5a) and Cas9 expression plasmid for cleaving pFA6 series deletion cassettes, including KanMX, hphMX and natMX. Used to perform CRISPR-Swap of alleles.DepositorInsert20mer CASS5a guide (gCASS5a) and 5' sgRNA
ExpressionBacterial and YeastAvailable SinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1 human HUWE1 WWE (1611-1703)
Plasmid#132614Purposeexpresses human HUWE1 WWE (1611-1703) in E. coliDepositorInsertHUWE1 WWE (1611-1703)
ExpressionBacterialAvailable SinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
NET30 pEGFP-N2 (815)
Plasmid#62011Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable SinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
SNAP-MIDAS
Plasmid#171604PurposeExpresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) in bacteria.DepositorInsertMIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)
TagsHis6 tag and SNAP tagExpressionBacterialAvailable SinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA6-LRRC52-V5-Flag-His
Plasmid#127818PurposeExpression of human LRRC52 in mammalian cellsDepositorAvailable SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only