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pTXB1-TnY

Plasmid

#163975

Purpose

IPTG-inducible expression of Transposon TnY fused to Mxe Intein and Chitin-binding domain.

Depositor

Peter Smibert

Article

Liscovitch-Brauer et al Nat Biotechnol. 2021 Apr 29. pii: 10.103

Insert

TnY

Tags

Mxe intein - Chitin-binding domain

Expression

Bacterial

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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Burden Monitor lambda version

Plasmid

#66073

Purpose

The burden monitor was cloned inside plasmid pAH63 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cells

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

Insert

GFP

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A

Plasmid

#83485

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R472A_H477A_R1048A_H1053A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R472A, H477A, R1048A, H1053A

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pBS4S

Plasmid

#55170

Purpose

Empty vector, integration at thrC, ampr, specr

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Bacillus biobrick box

Promoter

none

Available Since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only
pUASTattB-dALFANb(5mut)-mGreenLantern

Plasmid

#219661

Purpose

Gal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 5 destabilising mutations) fused to mGreenLantern

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

Destabilised ALFA nanobody (5 mutations) fused to mGreenLantern

Expression

Insect

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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p3869 HA-E6-AP C833A

Plasmid

#8649

Depositor

Peter Howley

Article

Talis et al J Biol Chem 1998 Mar 13;273(11):6439-45.

Insert

E6-AP (UBE3A Human)

Tags

HA

Expression

Mammalian

Mutation

C833A; active site Cys mutated to encode Ala

Available Since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only
HSC1-GiP

Plasmid

#58254

Purpose

This self-inactivated retroviral vector has an internal human EF1alpha promoter driving the expression of a bicistronic EGFP ires Puromycin cassette

Depositor

James Ellis

Article

Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

Insert

Enhanced Green Fluorescence Protein, Puromycin resistance gene

Use

Retroviral

Expression

Mammalian

Promoter

Human EF1-alpha

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only

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SPDK3860

Plasmid

#199245

Purpose

Modified Tobacco rattle virus (TRV) RNA2 vector for editing of Nicotiana benthamiana Phytoene desaturase (PDS) gene

Depositor

Savithramma Dinesh-Kumar

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence

Use

T-dna vector

Promoter

pPEBV

Available Since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

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CAG sHRPb-FKBP-post mGRASP

Plasmid

#73146

Purpose

Small fragment of split HRP and FKBP fused to the extracellular terminus of the "post mGRASP" scaffold published by Magee, in which the majority of the NLG1 extracellular domain is deleted.

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPb-FKBP-post mGRASP

Tags

HA tag

Expression

Mammalian

Promoter

CAG

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pC0060 AspCas13 (B04) His6-TwinStrep-SUMO-BsaI

Plasmid

#115210

Purpose

Expresses AspCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

AspCas13

Tags

SUMO

Expression

Bacterial

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hL1

Plasmid

#89411

Purpose

mammalian expression of hL1

Depositor

Fritz Rathjen

Article

De Angelis et al EMBO J. 1999 Sep 1. 18(17):4744-53.

Insert

L1CAM (L1CAM Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pRevTRE-hygro-GFP-Crumbs3a WT

Plasmid

#195217

Purpose

Retroviral Tet response vector containing GFP tagged Crumbs3a

Depositor

David Bryant, Ben Margolis

Article

Schluter et al Mol Biol Cell. 2009 Nov;20(22):4652-63.

Insert

CRB3A (CRB3 Human)

Use

Retroviral

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pUASTattB-dALFANb(3mut)-mGreenLantern

Plasmid

#219662

Purpose

Gal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 3 destabilising mutations) fused to mGreenLantern

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

Destabilised ALFA nanobody (3 mutations) fused to mGreenLantern

Expression

Insect

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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Burden Monitor phi80 version

Plasmid

#66074

Purpose

The burden monitor was cloned inside plasmid pAH153 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cell

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

Insert

GFP

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNAintron-CHIKV-Envelope protein

Plasmid

#215700

Purpose

Produces the Chikungunya virus envelope protein E3-E2-6K-E1

Depositor

Melinda Brindley

Article

Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

Insert

CHIKV E3-E2-6K-E1

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-Gad65P-eGFP-3XNLS-WPRE-pA

Plasmid

#183804

Purpose

AAV vector to express nuclear localized eGFP from a Gad65 promoter

Depositor

Jonathan Ploski

Article

de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

Insert

eGFP-NLS

Use

AAV

Tags

NLS

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pEE491

Plasmid

#149292

Purpose

T-DNA encoding TRV2 with tRNA multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_tRNA_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos

Expression

Plant

Promoter

35S

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R1079A

Plasmid

#83494

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (R1079A- pre-crRNA processing mutant)

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R1079A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R1079A

Promoter

T7

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pEE531

Plasmid

#149290

Purpose

T-DNA encoding TRV2 with spacer multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_Spacer_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos

Expression

Plant

Promoter

35S

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R1048A_H1053A

Plasmid

#83484

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 2 inactive mutant).

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R1048A_H1053A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R1048A, H1053A

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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