We narrowed to 12,205 results for: 112

1. pGL3-MTS-ATP6-W68-L16-G1333C-UGI-Puro

   Plasmid

   #198838

   Purpose

   Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ATP6) by swapping Trp codon TGA with stop codon TAA in C6 cells

   Depositor

   Bin Shen

   Article

   Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

   Insert

   ATP6-W68-L16 TALEN
   

   Expression

   Mammalian

   Available Since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL3-MTS-ND4-W24-L18-G1333C-UGI-Puro

   Plasmid

   #198856

   Purpose

   Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND4) by swapping Trp codon TGA with stop codon TAA in C6 cells

   Depositor

   Bin Shen

   Article

   Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

   Insert

   ND4-W24-L18 TALEN
   

   Expression

   Mammalian

   Available Since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3-MTS-ND2-W30-L16-G1333C-UGI-Puro

   Plasmid

   #198852

   Purpose

   Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND2) by swapping Trp codon TGA with stop codon TAA in C6 cells

   Depositor

   Bin Shen

   Article

   Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

   Insert

   ND2-W30-L16 TALEN
   

   Expression

   Mammalian

   Available Since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL3-MTS-CYTB-W77-L15-G1333C-UGI-Puro

   Plasmid

   #198848

   Purpose

   Employ DdCBE-mediated mtDNA editing to ablate mtProtein(CYTB) by swapping Trp codon TGA with stop codon TAA in C6 cells

   Depositor

   Bin Shen

   Article

   Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

   Insert

   CYTB-W77-L15 TALEN
   

   Expression

   Mammalian

   Available Since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL3-MTS-COX1-W25-L15-G1333C-UGI-Puro

   Plasmid

   #198842

   Purpose

   Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX1) by swapping Trp codon TGA with stop codon TAA in C6 cells

   Depositor

   Bin Shen

   Article

   Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

   Insert

   COX1-W25-L15 TALEN
   

   Expression

   Mammalian

   Available Since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL3-MTS-ND6-W82-L15-G1333N-UGI-Puro

   Plasmid

   #198860

   Purpose

   Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND6) by swapping Trp codon TGA with stop codon TAA in C6 cells

   Depositor

   Bin Shen

   Article

   Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

   Insert

   ND6-W82-L15 TALEN
   

   Expression

   Mammalian

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pD649-HAsp-NECTIN2-COMP5AP-AviTag-9xHis

   Plasmid

   #157331

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   NECTIN2 (NECTIN2 Human)
   

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. MDM4_Deletion_Downstream_gRNA_2

   Plasmid

   #195137

   Purpose

   gRNA in a third generation gRNA backbone with mCherry, targeting region immediately downstream of MDM4, to be used with MDM4_Deletion_Upstream_gRNA_1/2 for MDM4 deletion

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   MDM4 Deletion Downstream gRNA 2 (MDM4 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. MDM4_CRISPRi_gRNA_2

   Plasmid

   #195133

   Purpose

   gRNA from the Weissman CRISPRi library targeting MDM4, cloned into a third generation gRNA backbone with mCherry

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   MDM4 CRISPRi gRNA 2 (MDM4 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. MDM4_CRISPRi_gRNA_1

    Plasmid

    #195132

    Purpose

    gRNA from the Weissman CRISPRi library targeting MDM4, cloned into a third generation gRNA backbone with mCherry

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    MDM4 CRISPRi gRNA 1 (MDM4 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. MDM4_Deletion_Downstream_gRNA_1

    Plasmid

    #195136

    Purpose

    gRNA in a third generation gRNA backbone with mCherry, targeting region immediately downstream of MDM4, to be used with MDM4_Deletion_Upstream_gRNA_1/2 for MDM4 deletion

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    MDM4 Deletion Downstream gRNA 1 (MDM4 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMT-FLAG-CBPΔN

    Plasmid

    #182840

    Purpose

    A 5.2 kb Drosophila CBP cDNA fragment (cut by Nhe I and Nsi I), encoding C-terminal residues 1476-3222, was inserted into the pMT-FLAG vector. Expression in S2 cells by CuSO4 induction.

    Depositor

    Peter Harte

    Article

    Tie et al Development. 2009 Sep;136(18):3131-41. d

    Insert

    CBP cDNA fragment (cut by Nhe I and Nsi I) (nej Fly)
    

    Tags

    FLAG tag

    Expression

    Insect

    Promoter

    MT

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221-ZNF385A

    Plasmid

    #101615

    Purpose

    Donor Vector containing ZNF385A transcription factor, part of the Human TFome Collection

    Depositor

    George Church

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF385A (ZNF385A Human)
    

    Use

    Gateway shuttling vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOmnibac_ZZ_linker_K490_GFP_SNAP

    Plasmid

    #159721

    Purpose

    Expresses kinesin-1 (amino acids 1-490) in Sf9 cells with a C-terminal GFP and SNAP tag

    Depositor

    Ahmet Yildiz

    Article

    Ferro et al Science. 2022 Jan 21;375(6578):326-331.

    Insert

    KIF5B (only amino acids 1-490), K490 (KIF5B Human)
    

    Tags

    GFP and SNAP tags and ZZ affinity tag

    Expression

    Insect

    Mutation

    Only amino acids (1-490)

    Available Since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOmnibac_ZZ_linker_ybbR_MAP7_N

    Plasmid

    #159728

    Purpose

    Expresses MAP7 (amino acids 1-316) in Sf9 cells with a ZZ affinity tag and a ybbR tag

    Depositor

    Ahmet Yildiz

    Article

    Ferro et al Science. 2022 Jan 21;375(6578):326-331.

    Insert

    MAP7 (only amino acids 1-316)(includes MTBD and P123 domains) (MAP7 Human)
    

    Tags

    ZZ affinity tag and ybbR tag

    Expression

    Insect

    Available Since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET3d-SF-DCTN1-TS

    Plasmid

    #169375

    Purpose

    E. coli expression plasmid for human DCTN1 (longest transcript variant p150glued/isoform 1), with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.

    Depositor

    Bernd Bukau

    Article

    Bertolini et al Science. 2021 Jan 1;371(6524):57-64. doi

    Insert

    dynactin subunit 1 isoform 1 (DCTN1 Human)
    

    Tags

    6xHis Tag, SplitFlAsH Tag, and TwinStrep Tag

    Expression

    Bacterial

    Mutation

    Point mutations: A139T, I304T, Q322R, T516A, E577…

    Available Since

    May 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Venus-Puma-4E-pEGFP-C1

    Plasmid

    #166746

    Purpose

    Express Venus fused to the N-terminus of the Bcl-2 family protein, Puma with mutation in BH3 domain to disrupt binding anti-apoptotic proteins

    Depositor

    David Andrews

    Article

    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

    Insert

    Puma-4E (BBC3 Human)
    

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    4 hydrophobic residues in the BH3 region mutated …

    Promoter

    CMV

    Available Since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Venus-tBid-2E-pEGFP-C1

    Plasmid

    #166747

    Purpose

    Express Venus fused to the N-terminus of the Bcl-2 family protein, tBid with mutation to 2 positions in the BH3 domain

    Depositor

    David Andrews

    Article

    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

    Insert

    tBid-2E (Bid Mouse)
    

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    2 hydrophobic residues in the BH3 region mutated …

    Promoter

    CMV

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. sgRNA targeting human SLC12A5 gene stop codon

    Plasmid

    #158577

    Purpose

    For generate double-strand DNA break near the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.

    Depositor

    Rudolf Jaenisch

    Article

    Tang et al Sci Transl Med. 2019 Jul 31;11(503). pii

    Insert

    sgRNA targeting human SLC12A5 gene stop codon
    

    Use

    CRISPR

    Tags

    Cas9 and GFP

    Expression

    Mammalian

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-sACE2v2(732)

    Plasmid

    #154099

    Purpose

    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (untagged)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease and neck domains …

    Promoter

    CMV

    Available Since

    June 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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