We narrowed to 24,947 results for: CHI;
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Plasmid#223157PurposeExpression of KRAB with dSaCas9 and empty gRNA scaffoldDepositorInsertKRAB
UseAAV and CRISPRTags3xFLAGExpressionMammalianPromoterEF1aAvailable SinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRetro-UMI
Plasmid#222686PurposeCRISPR-screeningDepositorTypeEmpty backboneUseCRISPR and RetroviralExpressionMammalianMutationPGK without BbsI cutsiteAvailable SinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tol2-U6.3-sgRNA-empty-GFP
Plasmid#221843Purposeempty vector to clone custom sgRNA into BsaI sites to express sgRNA from chick U6.3 promoter expresses GFP reporter from GAGC promoterDepositorInsertEGFP
UseCRISPR; Tol2 transposon optimised for chick expre…ExpressionMammalianPromoterCAGCAvailable SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGM258
Plasmid#220176PurposeExpresses exogenous C-terminal mutant TTC1 (I271D)DepositorAvailable SinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
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BS-ZJLEU-PTEF1-HFL1-D384A Y387A-GFP
Plasmid#207024PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
TagsGFPExpressionYeastMutationD384A Y387APromoterpTEF1Available SinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-W371A I375A-GFP
Plasmid#207023PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
TagsGFPExpressionYeastMutationW371A I375APromoterpTEF1Available SinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-Y387A-GFP
Plasmid#207022PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
TagsGFPExpressionYeastMutationY387APromoterpTEF1Available SinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-D384A-GFP
Plasmid#207021PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
TagsGFPExpressionYeastMutationD384APromoterpTEF1Available SinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-I375A-GFP
Plasmid#207020PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
TagsGFPExpressionYeastMutationI375APromoterpTEF1Available SinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-W371A-GFP
Plasmid#207019PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
TagsGFPExpressionYeastMutationW371APromoterpTEF1Available SinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ypq2-2GFP-URA
Plasmid#207010PurposeIntegration of 2xGFP tag at YPQ2 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane.DepositorInsertYPQ2
Tags2xGFPExpressionYeastAvailable SinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
Vph1-2GFP-URA
Plasmid#207009PurposeIntegration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane.DepositorInsertVPH1
Tags2xGFPExpressionYeastAvailable SinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMCSG7-10xHis-ecRNHI*
Plasmid#219523Purposebacterial expression of cysteine-free variant of the E. coli ribonuclease HI geneDepositorInsertcysteine-free variant of the E. coli ribonuclease HI gene
TagsN terminal 10xHis TagExpressionBacterialMutationCysteine free variantAvailable SinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGM268
Plasmid#220181PurposeExpresses exogenous C-terminal mutant TTC1 (I271D) with an N-terminal sfGFP tagDepositorAvailable SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-IFT22
Plasmid#218720PurposeExpresses N-terminally EGFP-tagged IFT22 in mammalian cellsDepositorAvailable SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGM216
Plasmid#220175PurposeExpresses exogenous TPR mutant TTC1 (R190A_R194A)DepositorAvailable SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ-shWT1_A
Plasmid#217636PurposeDox inducible shRNA targeting WT-1DepositorInsertshWT1_A (WT1 Human)
UseRNAiAvailable SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pToxEXPr6
Plasmid#218290PurposeIntroduction of a cyanamide-inducible I-SceI expression cassette and a TetR-TUP1 expression cassette, use together with pJQR3DepositorInserthph>tCYC1-ISceI.site-Repeat2-pDDI2>ISceI>tSynth3-pHAC1>TetR>TUP1>tABF1-HO(182,1979)
ExpressionYeastAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJQR3
Plasmid#218289PurposeIntroduction of a Zif268-hER-VP16 expression cassette and a LmrA-Mig1C expression cassette, use together with pToxEXPR6DepositorInsertHO(-253,-1)-pADH1>LmrA>MIG1(aa123-504)>tMIG1-tYGL036WExpressionYeastAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only