We narrowed to 20,122 results for: Kin

1. pX462-hPRKAA1-gRNA_A

   Plasmid

   #74374

   Purpose

   gRNA_A to knockout human AMPK alpha 1 using Cas9n

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   human AMPK alpha 1 exon1 gRNA (PRKAA1 Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pProEx Rap1A(G12C, L96F) CL

   Plasmid

   #224264

   Purpose

   Protein purification of Rap1A (G12C, L96F)

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   Rap1A (RAP1A Human)
   

   Expression

   Bacterial

   Mutation

   G12C, L96F

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBSFI-myr-akt-T308A/S473A

   Plasmid

   #49191

   Purpose

   cDNA of Akt1 with myristyolation signal and point mutation from T to A at position 308 and S to A at position 473 in cloning vector

   Depositor

   Peter Vogt

   Article

   Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Adaptor plasmid

   Tags

   HA tag and myristolation signal

   Mutation

   T308A; S473A

   Promoter

   T7

   Available Since

   Dec. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)-HLA-cmyc-natT1R2

   Plasmid

   #113946

   Purpose

   mammalian expression plasmid for c-myc-tagged human T1R2 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R2 (TAS1R2 Human)
   

   Tags

   Signal/leader sequence from HLA class I histocomp…

   Expression

   Mammalian

   Mutation

   residues 22-839

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. lentiCRISPRv2.sgNf1.1

   Plasmid

   #91895

   Purpose

   sgNf1.1 for Nf1 deletion

   Depositor

   Wen Xue

   Article

   Song et al Gastroenterology. 2017 Apr;152(5):1161-1

   Insert

   sgNf1.1
   

   Use

   Lentiviral and Mouse Targeting

   Expression

   Mammalian

   Available Since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLCHKOv3-mCherry

   Plasmid

   #209035

   Purpose

   Lentiviral backbone for expressing U6 driven hybrid guide (hg)RNAs with BveI cloning sites, mCherry and puromycin selection marker

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiCRISPRv2.sgNf1.4

   Plasmid

   #92029

   Purpose

   sgNf1.4 for Nf1 deletion

   Depositor

   Wen Xue

   Article

   Song et al Gastroenterology. 2017 Apr;152(5):1161-1

   Insert

   sgNf1.4 for Nf1 deletion
   

   Use

   Lentiviral and Mouse Targeting

   Expression

   Mammalian

   Available Since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSP64-hKCNQ1

   Plasmid

   #53048

   Purpose

   Expresses alpha subunit for hKCNQ1 channel in Xenopus oocytes

   Depositor

   Michael Sanguinetti

   Article

   Sanguinetti et al Nature. 1996 Nov 7;384(6604):80-3.

   Insert

   Potassium voltage-gated channel subfamily KQT member 1 (KCNQ1 Human)
   

   Use

   Xenopus oocyte expression

   Promoter

   SP6

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. FLAG-MED7

   Plasmid

   #15416

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

   Insert

   MED7 (MED7 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N/A

   Available Since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pTag-BFP-C-h-Rab4a-c-Myc

    Plasmid

    #79799

    Purpose

    Express TagBFP labeled human Rab4a (GTPase located on recycling endosomal membranes).

    Depositor

    James Johnson

    Article

    Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

    Insert

    Rab4a (RAB4A Human)
    

    Tags

    TagBFP and myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. TTR C10S S46C

    Plasmid

    #190008

    Purpose

    Expression of human TTR in E coli

    Depositor

    Peter Wright

    Article

    Sun et al J Biol Chem. 2022 Jun 18;298(8):102162.

    Insert

    TTR (TTR Human)
    

    Expression

    Bacterial

    Mutation

    C10S, S46C

    Promoter

    T7

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. 2_T7-mScarlet3-KRas

    Plasmid

    #225929

    Purpose

    In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the K-Ras membrane localisation signal

    Depositor

    Michalis Averof

    Article

    Karapidaki et al bioRxiv 2024.11.12.623055

    Insert

    mScarlet3-KRas
    

    Use

    In vitro transcription

    Promoter

    T7

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEMHE-mClover3-KASH5-DN

    Plasmid

    #171492

    Purpose

    T7 promotor drives in vitro transcription of mClover3-tagged bovine Kash5 DN mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    KASH5-DN (CCDC155 B. tarus (bovine))
    

    Use

    Pgehme

    Tags

    mClover3

    Promoter

    T7

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEMHE-GST-KASH5-DN

    Plasmid

    #171493

    Purpose

    T7 promotor drives in vitro transcription of GST-tagged bovine Kash5 DN mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    KASH5-DN (CCDC155 B. tarus (bovine))
    

    Use

    Pgehme

    Tags

    GST

    Promoter

    T7

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR221-eGFP_mito

    Plasmid

    #221414

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    eGFP_mito
    

    Expression

    Mammalian

    Available Since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. PGK ARF6(Q67L)-CFP

    Plasmid

    #200428

    Purpose

    Mammalian expression of Q67L mutation in ARF6

    Depositor

    Erika Holzbaur

    Article

    Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

    Insert

    ARF6 (ARF6 Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Q67L

    Promoter

    PGK

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCK301

    Plasmid

    #87767

    Purpose

    E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest to express using L-rhamnose, three terminators, ampR, pBR322 origin, lacI

    Depositor

    John Heap

    Article

    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1

    Insert

    PrhaBAD-sfGFP
    

    Use

    Synthetic Biology

    Tags

    6xHis Tag

    Expression

    Bacterial

    Promoter

    PrhaBAD rhamnose-inducible promoter from E. coli

    Available Since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL3-IRES-miR34a-P2

    Plasmid

    #64786

    Purpose

    Luciferase expression driven by miR-34a promoter P2

    Depositor

    Joshua Mendell

    Article

    Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

    Insert

    miR-34a promoter P2 (MIR34A Human)
    

    Use

    Luciferase

    Available Since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. Dnase1L3 R206C pIRES-DsRed

    Plasmid

    #98847

    Purpose

    Expresses Human Dnase1L3 with activity-reducing HUVS mutation R206C in mammalian cells along with DsRed

    Depositor

    Peter Keyel

    Article

    Shi et al Front Immunol. 2017 May 8;8:522. doi: 10

    Insert

    Dnase1L3 (DNASE1L3 Human)
    

    Tags

    IRES DsRed

    Expression

    Mammalian

    Mutation

    R206C which greatly reduces nuclease activity

    Promoter

    CMV

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. DRH323: pAAV_hSyn-QuasAr2

    Plasmid

    #107705

    Purpose

    in vivo voltage imaging

    Depositor

    Adam Cohen

    Article

    Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

    Insert

    QuasAr2
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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