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pDEST40-ARF1-V5-His6

Plasmid

#67432

Purpose

Mammalian expression of hArf1 with C-term V5 and His6 tag

Depositor

Richard Kahn

Publication

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF1 (ARF1 Human)

Tags

His and V5

Expression

Mammalian

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL14-GFP

Plasmid

#67406

Purpose

Mammalian expression of hArl14 with C-term GFP tag

Depositor

Richard Kahn

Publication

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL14 (ARL14 Human)

Tags

GFP

Expression

Mammalian

Mutation

bp 172 C to T; aa L58F

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDSHA-ARF6-Myc

Plasmid

#67352

Purpose

Mammalian expression of hArf6 with C-term Myc tag

Depositor

Richard Kahn

Publication

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF6 (ARF6 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDSHA-ARL1-Myc

Plasmid

#67353

Purpose

Mammalian expression of hArl1 with C-term Myc tag

Depositor

Richard Kahn

Publication

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL1 (ARL1 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDSHA-ARF3-Myc

Plasmid

#67349

Purpose

Mammalian expression of hArf3 with C-term Myc tag

Depositor

Richard Kahn

Publication

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF3 (ARF3 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
p3EHA

Plasmid

#67708

Purpose

3' entry HA tag

Depositor

Nathan Lawson

Publication

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

HA tag

Tags

HA

Promoter

None

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pDRFLIP34

Plasmid

#65512

Purpose

FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Publication

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-AFPt9-attR1 and attR2-t7TFPt9-cMyc

Expression

Yeast

Promoter

pPMA1

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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Luc-Nanog-3'UTR

Plasmid

#63893

Purpose

Luciferase reporter of mouse wildtype Nanog 3'UTR

Depositor

Lin He

Publication

Choi et al Nat Cell Biol. 2011 Oct 23;13(11):1353-6

Insert

Nanog homeobox (Nanog Mouse)

Expression

Mammalian

Available since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFRT-DestFLAGHA_RBPMS

Plasmid

#65743

Purpose

constitutively expresses RBPMS in mammalian cells

Depositor

Thomas Tuschl

Publication

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

FLAG HA

Expression

Mammalian

Promoter

CMV, 2x Tet

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only
pDEST26 Ago2 S387A

Plasmid

#59905

Purpose

Expresses 6xHis-Ago2 (N-termi tag) S387A mutant in mammalian cells

Depositor

Carl Novina

Publication

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Tags

6xHis (N-termi tag)

Expression

Mammalian

Mutation

S387A, phospho-null alanine

Promoter

CMV

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pJW4.16

Plasmid

#62871

Purpose

Bacterial expression of MBP-MBK-2

Depositor

Geraldine Seydoux

Publication

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

MBP-MBK-2

Tags

MBP

Expression

Bacterial

Available since

April 22, 2015

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHA_RBPMS

Plasmid

#59388

Purpose

Destination vector with RBPMS for stable cell line generation

Depositor

Thomas Tuschl

Publication

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

FLAG HA

Expression

Mammalian

Promoter

CMV, 2x Tet

Available since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pInducer21 Flag-Sesn2

Plasmid

#61829

Purpose

Dox inducible lentivirial expression of Flag-Sesn2 (mouse)

Depositor

Ming Li

Publication

Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

Insert

Sesn2 (Sesn2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only
LIIbeta F 1-2 RNAi

Plasmid

#54403

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Type

Empty backbone

Use

Plant expression

Available since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only

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LIIalpha F 1-2 RNAi

Plasmid

#54404

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Type

Empty backbone

Use

Plant expression

Available since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pGL4.23 C3_9

Plasmid

#60295

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Publication

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

NKX6-1 enhancer (NKX6-1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
p41Neo MagicFluor

Plasmid

#58564

Purpose

p41Neo carrying both mating type markers buffered by the Cyc1 terminator

Depositor

Leonid Kruglyak

Insert

Ste2p-Citrine, Cyc1 term, Ste3p-mCherry

Expression

Yeast

Available since

Nov. 24, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDEST-hPDIb′a′

Plasmid

#60556

Purpose

enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli

Depositor

Han Choe

Publication

Song et al Appl Biochem Biotechnol. 2013 May;170(1)

Type

Empty backbone

Tags

His6-hPDIb'a' tag

Expression

Bacterial

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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PNXGW

Plasmid

#48256

Purpose

Split-GFP assay in plants

Depositor

Wolf Frommer

Publication

Kim et al Plant Cell. 2009 Apr;21(4):1305-23. doi:

Type

Empty backbone

Use

Binary gateway vector

Tags

6xHIS and YFP aa1-172

Promoter

35S

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only
CCT8_pCSdest

Plasmid

#53795

Depositor

Roger Reeves

Publication

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

CCT8 (CCT8 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available since

Sept. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits