We narrowed to 1,174 results for: Aspa

1. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-8

   Plasmid

   #129048

   Purpose

   inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA8 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA8 (huTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-1

   Plasmid

   #129041

   Purpose

   inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA1 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA1 (huTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. N295A (EcN), N229A (EcQ) MetNIQ in pBAD

   Plasmid

   #118581

   Purpose

   N295A Mutation of MetN in Methionine ABC Transporter and N229A Mutation in Methionine Binding Protein (MetQ) for Transport Activity Assay

   Depositor

   Douglas Rees

   Article

   Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

   Inserts

   MetN (metN E. coli)
   MetI (metI E. coli)
   MetQ (metQ E. coli)
   

   Use

   Tags

   10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

   Expression

   Bacterial

   Mutation

   Changed Asparagine (Asn) 229 to Alanine (Ala) and…

   Promoter

   None and araBAD Promotoer

   Available since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX304-EZH1-D745N

   Plasmid

   #203585

   Purpose

   Expresses V5-tagged mutant version of EZH1 partially resistant to JQ-EZ-05 in mammalian cells.

   Depositor

   Abhishek Chakraborty, William Kaelin

   Article

   Chakraborty et al Sci Transl Med. 2017 Jul 12;9(398):eaal5

   Insert

   EZH1 (EZH1 Human)
   

   Use

   Lentiviral

   Tags

   V5-tagged

   Expression

   Mammalian

   Mutation

   changed Aspartic Acid 745 to Asparagine for parti…

   Promoter

   CMV

   Available since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Myc-Nix-S212D

   Plasmid

   #197515

   Purpose

   Mammalian expression of myc-tagged human Nix with an aspartic acid substitution of serine-212. Based on Myc-Nix (#100795)

   Depositor

   Joseph Gordon

   Article

   da Silva Rosa et al Autophagy. 2021 Sep;17(9):2257-2272. doi

   Insert

   BCL2 interacting protein 3 like (BNIP3L Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Serine 212 changed to aspartic acid

   Promoter

   CMV

   Available since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT2iApowriter2(#336)

   Plasmid

   #184104

   Purpose

   cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish transient transgenic

   Depositor

   Sophie Vriz

   Article

   Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

   Insert

   myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
   

   Use

   Zebrafish transient transgenesis

   Tags

   internal tag: 5myc

   Expression

   Mutation

   DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

   Promoter

   Zf-Ubi

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT22Apowriter2(#340)

   Plasmid

   #184105

   Purpose

   cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

   Insert

   myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   internal tag: 5myc

   Expression

   Mutation

   DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

   Promoter

   Zf-Ubi

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT22iuasvApoWtEos3N(#486)

   Plasmid

   #184107

   Purpose

   cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

   Insert

   myr-DIAP1-2nls-tEosFP-nls'-P2A-Casp9-ERT2
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   Expression

   Mutation

   DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

   Promoter

   4xUAS

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT22iuasvApoWChe(#488)

   Plasmid

   #184108

   Purpose

   cyclofen-inducible apoptosis activation and imaging (mCherry) in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

   Insert

   myr-DIAP1-nls'-mCherry-P2A-Casp9-ERT2
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   Expression

   Mutation

   DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

   Promoter

   4xUAS

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. CPH3569 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,K1487N,D1497K; msLink2)

    Plasmid

    #91820

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Use

    Tags

    10xHis

    Expression

    Yeast

    Mutation

    5xGly linker, PreScission Protease site (LEVLFQGP…

    Promoter

    Endogenous Rpb1 promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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