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Plasmid#183086Purpose2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assemblyDepositorInsertO2A
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYTK001_P2At(2nd)
Plasmid#183087Purpose2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assemblyDepositorInsertP2A
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-bhaA
Plasmid#167810PurposeExpresses His6-tagged BhaA in E. coliDepositorInsertBhaA
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-bhaB1
Plasmid#167811PurposeExpresses His6-tagged BhaB1 in E. coliDepositorInsertBhaB1
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-bhaB7
Plasmid#167813PurposeExpresses His6-tagged BhaB7 in E. coliDepositorInsertBhaB7
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15b-bhaC1
Plasmid#167814PurposeExpresses BhaC1 in E. coliDepositorInsertBhaC1
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pET30a-RAMA
Plasmid#112226PurposeExpresses RAMA domain from murine MPNDDepositorInsertMPND (Mpnd Mouse)
UseTagsFlag and HIs S-tagExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMDC84-ScSEIPIN
Plasmid#96984PurposeExpress yeast SEIPIN gene in plants.DepositorInsertScSEIPIN (SEI1 Budding Yeast)
UseTags6xHis and GFPExpressionPlantMutationPromoter35SAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pstb-LAFR5-exoY
Plasmid#86170Purposepstb-LAFR5 with the S. meliloti 1021 exoY fragment from E6 cloned into Pst/BamDepositorInsertexoY
UseTagsExpressionBacterialMutationPromoterSmb21651Available sinceFeb. 6, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-Gm1
Plasmid#53528PurposeExpresses a plant arginyl-tRNA synthetase in E.coliDepositorInsertarginyl-tRNA synthetase
UseTagsHis Tag and ThioredoxinExpressionBacterialMutationPromoterT7Available sinceJuly 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET20b- hTPI Ile170Thr
Plasmid#50725Purposeoverexpression of the human TPI1 Ile170Thr allele in E.coliDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
UseTags6xHIS tagExpressionBacterialMutationchanged Isoleucine 170 to ThreoninePromoterT7Available sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
HA GSK3 beta wt pcDNA3
Plasmid#14753PurposeMammalian expression of human GSK3 beta with HA tagDepositorInsertGSK3 beta (GSK3B Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 12, 2007AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJL1-CRM197-4xDQNAT
Plasmid#128395PurposeIn vitro expression of CRM197 with C terminal 4xDQNAT glycosylation sitesDepositorInsertDiphtheria toxin
UseTags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialMutationInactivating G52E mutationPromoterT7Available sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG-FLEx(FRT)-G
Plasmid#67828PurposeFlp-dependent expression of rabies glycoprotein to mediate trans-synaptic spread of modifed rabies virus in cellsDepositorInsertrabies glycoprotein
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
GAL-Aur1-2GFP-Hygromycin
Plasmid#184775PurposeYeast expression of Aur1 under GAL promoter. Uses antibiotic resistance marker hphMX6.DepositorInsertproGAL-Aur1 (AUR1 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-GCaMP6s-CAAX
Plasmid#52228PurposePlasma membrane targeted GCaMP6s, calcium sensorDepositorInsertGCaMP6s-CAAX
UseTags6xHis and CAAXExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-TVAmCherry-2A-oG
Plasmid#104330PurposeAAV constitutively expressing TVA-mCherry fusion and optimised Rabies Glycoprotein (oG)DepositorInsertsTVA-mCherry fusion protein
Optimised Rabies Glycoprotein (oG)
UseAAV; Adeno associated viral vectorTagsExpressionMutationPromoterAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTrEno
Plasmid#115474PurposeEno-promoter driven expression vector for protein production in Trichoderma reeseiDepositorInsertCel7A
UseUnspecifiedTagsExpressionMutationIntrons have been removedPromoterTrichoderma reesei Enolase (Eno) promoterAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMA3287
Plasmid#46883PurposeLentiviral vector encoding Tet-inducible mitochondrially targeted Y147A mutant human Uracil-N-glycosylaseDepositorInsertUracil-N-glycosylase, Y147A mutant (UNG Human)
UseLentiviralTagsMycExpressionMammalianMutationY147APromoterTRE-TightAvailable sinceAug. 14, 2013AvailabilityAcademic Institutions and Nonprofits only