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Showing: 1161 - 1180 of 8297 results

  2. pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196078
    Purpose
    (Empty Backbone) Constitutive CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Promoter
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196078-plasmid-map-sequence-id-388989

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  3. pSBbi-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196076
    Purpose
    (Empty Backbone) Constitutive CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Promoter
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196076-plasmid-map-sequence-id-388979

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  4. pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196074
    Purpose
    (Empty Backbone) Constitutive CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Promoter
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196074-plasmid-map-sequence-id-389001

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  5. SMYD3

    Plasmid
    #32048
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SMYD3 (SMYD3 Human)
    Use
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196082
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Aminoglycoside phosphotransferase
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Promoter
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196082-plasmid-map-sequence-id-388978

    Enlarge

  7. pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196080
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Blasticidin S deaminase
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Promoter
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196080-plasmid-map-sequence-id-388988

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  8. pMTB-Multibow-B

    Plasmid
    #60978
    Purpose
    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes cytoplasmic EBFP2.
    Depositor
    Sean Megason
    Article
    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do
    Insert
    EBFP2
    Use
    Zebrafish
    Tags
    Expression
    Mutation
    Promoter
    Bactin2
    Available since
    Jan. 22, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. pMTB-Multibow-R

    Plasmid
    #60983
    Purpose
    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes cytoplasmic dTomato
    Depositor
    Sean Megason
    Article
    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do
    Insert
    dTomato
    Use
    Zebrafish
    Tags
    Expression
    Mutation
    Promoter
    Bactin2
    Available since
    Feb. 3, 2015
    Availability
    Academic Institutions and Nonprofits only

  10. pMTB-Multibow-Y

    Plasmid
    #60981
    Purpose
    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes cytoplasmic citrine
    Depositor
    Sean Megason
    Article
    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do
    Insert
    citrine
    Use
    Zebrafish
    Tags
    Expression
    Mutation
    Promoter
    Bactin2
    Available since
    Jan. 22, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. pMTB-Multibow-fR

    Plasmid
    #60984
    Purpose
    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes cytoplasmic mKate2.
    Depositor
    Sean Megason
    Article
    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do
    Insert
    mKate2
    Use
    Zebrafish
    Tags
    Expression
    Mutation
    Promoter
    Bactin2
    Available since
    Jan. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    60984-plasmid-map-sequence-id-98502

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  12. pMTB-Multibow-G

    Plasmid
    #60980
    Purpose
    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes cytoplasmic EGFP
    Depositor
    Sean Megason
    Article
    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do
    Insert
    EGFP
    Use
    Zebrafish
    Tags
    Expression
    Mutation
    Promoter
    Bactin2
    Available since
    Jan. 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  13. pMTB-Multibow-C

    Plasmid
    #60979
    Purpose
    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes cytoplasmic cerulean
    Depositor
    Sean Megason
    Article
    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do
    Insert
    Cerulean
    Use
    Zebrafish
    Tags
    Expression
    Mutation
    Promoter
    Bactin2
    Available since
    Feb. 3, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. pMTB-Multibow-O

    Plasmid
    #60982
    Purpose
    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes cytoplasmic mKO
    Depositor
    Sean Megason
    Article
    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do
    Insert
    mKO
    Use
    Zebrafish
    Tags
    Expression
    Mutation
    Promoter
    Bactin2
    Available since
    Jan. 22, 2015
    Availability
    Academic Institutions and Nonprofits only

  16. pMTB-Multibow-hB

    Plasmid
    #60992
    Purpose
    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear EBFP2.
    Depositor
    Sean Megason
    Article
    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do
    Insert
    EBFP2
    Use
    Zebrafish
    Tags
    histone 2B tag
    Expression
    Mutation
    Promoter
    Bactin2
    Available since
    Jan. 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  17. pMTB-Multibow-hY

    Plasmid
    #60995
    Purpose
    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear citrine
    Depositor
    Sean Megason
    Article
    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do
    Insert
    citrine
    Use
    Zebrafish
    Tags
    histone 2B tag
    Expression
    Mutation
    Promoter
    Bactin2
    Available since
    Feb. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    60995-plasmid-map-sequence-id-98504

    Enlarge

  18. pMTB-Multibow-mfR

    Plasmid
    #60991
    Purpose
    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound mKate2.
    Depositor
    Sean Megason
    Article
    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do
    Insert
    mKate2
    Use
    Zebrafish
    Tags
    membrane tag
    Expression
    Mutation
    Promoter
    Bactin2
    Available since
    Jan. 22, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. pMTB-Multibow-mG

    Plasmid
    #60987
    Purpose
    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound EGFP
    Depositor
    Sean Megason
    Article
    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do
    Insert
    EGFP
    Use
    Zebrafish
    Tags
    membrane tag
    Expression
    Mutation
    Promoter
    Bactin2
    Available since
    Jan. 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. pMTB-Multibow-hR

    Plasmid
    #60997
    Purpose
    Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes nuclear dTomato
    Depositor
    Sean Megason
    Article
    Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do
    Insert
    dTomato
    Use
    Zebrafish
    Tags
    histone 2B tag
    Expression
    Mutation
    Promoter
    Bactin2
    Available since
    March 9, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 1161 - 1180 of 8297 results