We narrowed to 24,460 results for: Spr

1. pYPQAT41

   Plasmid

   #223413

   Purpose

   T-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants select.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-ecTadA8e-SpRY-D10A-OsU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTarget_PS_VA

   Plasmid

   #186454

   Purpose

   pTarget containing protospacer for FnCas12a targeting

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTarget_PS_VB

   Plasmid

   #186456

   Purpose

   pTarget containing protospacer for AaCas12b targeting

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAraQCas-BsaI

   Plasmid

   #170633

   Purpose

   Expression of CRISPR-associated transposases

   Depositor

   Sheng Yang

   Article

   Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

   Insert

   VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)
   

   Expression

   Bacterial

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUDE1084

   Plasmid

   #168500

   Purpose

   expression of T7RNA polymerase and CRISPR endonunclease Fncas12a

   Depositor

   Jean-Marc Daran

   Article

   Randazzo et al CRISPR J. 2021 Apr 23. doi: 10.1089/cris

   Inserts

   Fncas12a
   T7RNA polymerase
   

   Use

   CRISPR

   Promoter

   TDH3 and TEF1

   Available Since

   Dec. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTarget_priming_VA

   Plasmid

   #186455

   Purpose

   pTarget containing protospacer with a mismatch on position 1, for FnCas12a targeting

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pdCas12a(RuvC)

   Plasmid

   #186447

   Purpose

   Encodes catalytically inactive Cas12a (D917A, E1006A) from F. novicida (type V-A)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   dCas12a
   

   Use

   CRISPR

   Mutation

   D917A, E1006A

   Promoter

   T7

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pdCas12b(RuvC)

   Plasmid

   #186450

   Purpose

   Encodes catalytically inactive Cas12b (E848A, D977A) from A. acidoterrestris (type V-B)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   dCas12b
   

   Use

   CRISPR

   Mutation

   E848A, D977A

   Promoter

   T7

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCas4_1_2_VA

   Plasmid

   #186438

   Purpose

   Encodes Cas4, Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas4, Cas1 and Cas2
   

   Use

   CRISPR

   Promoter

   T7

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCas4_1_Δ2_VA

    Plasmid

    #186439

    Purpose

    Encodes Cas4, Cas1, leader, repeat and one spacer from F. Novicida (type V-A)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4, Cas1 and Cas2
    

    Use

    CRISPR

    Promoter

    T7

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCasΔ4_1_2_VA

    Plasmid

    #186440

    Purpose

    Encodes Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas1 and Cas2
    

    Use

    CRISPR

    Promoter

    T7

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCas-mut4_1_2_VA

    Plasmid

    #186441

    Purpose

    Encodes Cas4 (K70A), Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4, Cas1 and Cas2
    

    Use

    CRISPR

    Promoter

    T7

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCas-4/1_2_VB

    Plasmid

    #186442

    Purpose

    Encodes Cas4/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4/1 and Cas2
    

    Use

    CRISPR

    Promoter

    T7

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCas-4/1Δ2_VB

    Plasmid

    #186443

    Purpose

    Encodes Cas4/1, leader, repeat and one spacer from A. acidoterrestris (type V-B)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4/1
    

    Use

    CRISPR

    Promoter

    T7

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCas12(PI)

    Plasmid

    #186448

    Purpose

    Encodes inactive Cas12a containing mutation in the PI domain (K613A, K617A) from F. novicida (type V-A)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas12a (PI)
    

    Use

    CRISPR

    Mutation

    K613A, K617A

    Promoter

    T7

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCas12b(PI)

    Plasmid

    #186451

    Purpose

    Encodes inactive Cas12b containing mutation in the PI domain (R122A, G143P) from A. acidoterrestris (type V-B)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas12b (PI)
    

    Use

    CRISPR

    Mutation

    R122A, G143P

    Promoter

    T7

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCas-mut4/1_2_VB

    Plasmid

    #186444

    Purpose

    Encodes Cas4(K81A)/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4/1 and Cas2
    

    Use

    CRISPR

    Mutation

    Cas4 domain (K81A)

    Promoter

    T7

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCas_4(I-U)/1_2_VB

    Plasmid

    #186445

    Purpose

    Encodes Cas4/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B). Cas4 domain swapped with type U-I Cas4 domain from G. sulfurreducens

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4/1 and Cas2
    

    Use

    CRISPR

    Mutation

    Cas4 domain swapped with Cas4 domain from G. sulf…

    Promoter

    T7

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAIP4_1TJ6

    Plasmid

    #234167

    Purpose

    Heterologous protein expression of sprouty-related, EVH1 domain-containing protein 1 (Spred-1) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    1TJ6 (spred1 Xenopus tropicalis)
    

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. PAJ389

    Plasmid

    #104147

    Purpose

    dCas9 plasmid expressing 2A sRNA trigger

    Depositor

    Alfonso Jaramillo

    Article

    Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    pLtetO-1

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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