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pcDNA3-FLAG/MKRN1(110-482)

Plasmid

#78754

Purpose

To overexpress MKRN1(110-482) in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

Insert

MKRN1 (MKRN1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

110-482

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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GST-PC-2-CTS

Plasmid

#86068

Purpose

A bacterial expression plasmid encoding polycystin-2 ciliary targeting signal with N-terminus GST-tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Polycystin-2 (Pkd2 Human)

Tags

GST

Expression

Bacterial

Mutation

contains the region from 1-15

Promoter

tac

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-eBE-S1

Plasmid

#101092

Purpose

Expresses eBE-S1 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Cell Res. 2017 Aug 29. doi: 10.1038/cr.2

Insert

eBE-S1

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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P2RX6-bio-His

Plasmid

#53360

Purpose

Expresses full-length P2X purinoceptor 6 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

P2RX6 (P2RX6 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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mEmerald-PCNA-19-SV40NLS-4

Plasmid

#54223

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Lu et al J Cell Biol. 2010 Jun 14;189(6):967-80.

Insert

PCNA-19 SV40NLS (PCNA Human)

Tags

3xSV40NLS and mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
ENDOD1-bio-His

Plasmid

#53155

Purpose

Expresses full-length ENDOD1 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

ENDOD1 (ENDOD1 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-SH3BP4 W92A

Plasmid

#46893

Purpose

expresses HA tagged SH3BP4 W92A mutant in mammalian cells

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2012 Jun 29;46(6):833-46. doi:

Insert

SH3BP4 W92A (SH3BP4 Human)

Tags

HA

Expression

Mammalian

Mutation

W92A (mutated SH3 domain)

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pGEX RalB G23V ΔC

Plasmid

#50995

Purpose

Expresses RalB G23V ΔC in bacterial cells

Depositor

Anna Sablina

Article

Simicek et al Nat Cell Biol. 2013 Oct;15(10):1220-30.

Insert

RALB (RALB Human)

Tags

GST

Expression

Bacterial

Mutation

G23V ΔC

Promoter

tac_promoter

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX Itk TEC

Plasmid

#20711

Depositor

Leslie Berg

Article

Bunnell et al J Biol Chem. 2000 Jan 21. 275(3):2219-30

Insert

Itk TEC (Itk Mouse)

Tags

GST

Expression

Bacterial

Mutation

amino acids 97-174

Available Since

March 17, 2009

Availability

Academic Institutions and Nonprofits only
3603 pGEX4T-1 Bad S112A (104-141)

Plasmid

#8802

Depositor

Stanley Korsmeyer

Article

Harada et al Mol Cell 1999 Apr;3(4):413-22.

Insert

Bad (Bad Mouse)

Tags

GST

Expression

Bacterial

Mutation

Contains AA's 104-141; S112A

Promoter

Ptac

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pEF Itk P158A P159A myc

Plasmid

#20708

Depositor

Leslie Berg

Article

Bunnell et al J Biol Chem. 2000 Jan 21. 275(3):2219-30

Insert

Itk PR (Itk Mouse)

Tags

myc

Expression

Mammalian

Mutation

P158A P159A. Inactivates the proline-rich region.

Available Since

March 17, 2009

Availability

Academic Institutions and Nonprofits only
LB1360 (Bcl-2 promoter with MHS4)

Plasmid

#15392

Depositor

Linda Boxer

Article

Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial and Mammalian

Mutation

Bcl-2 full promoter with IgH enhancers MHS4

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pPBbsr2-miRFP703-dSal-p53NT(1-97)-S15D-hCRY2-NLS

Plasmid

#241842

Purpose

Expresses an actuator of Opto-p53 in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Tsuruoka et al Cell Struct Funct. 2025 Jun 12. doi: 10.

Insert

p53 (TP53 Human)

Tags

CRY2 and miRFP703

Expression

Mammalian

Mutation

N-terminus (1-97 aa) containing S15D

Promoter

CAGGS promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pGIPZ EF1a spnull-3xFLAG-DR18 IRES mNeonGreen-P2A-Puro

Plasmid

#248037

Purpose

Constitutively expresses 3xFLAG tagged DR18, mNeonGreen and a puromycin resistance gene in human cells

Depositor

Joshua Leonard

Article

Deol et al GEN Biotechnol. 2023 Jun 1;2(3):228-246.

Insert

3xFLAG-tagged DR18

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

human EF1a

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pGIPZ EF1a ACCx2 uORF eIL12 IRES mNeonGreen-P2A-Puro

Plasmid

#248035

Purpose

Constitutively expresses an engineered version of IL12, mNeonGreen and a puromycin resistance gene in human cells

Depositor

Joshua Leonard

Article

Deol et al GEN Biotechnol. 2023 Jun 1;2(3):228-246.

Insert

Engineered IL12

Use

Lentiviral

Expression

Mammalian

Promoter

human EF1a

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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iPE-D10A-Cas9

Plasmid

#239380

Purpose

For inverse prime editor using nCas9-D10A in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:

Insert

nCas9-D10A, M-MLV RT

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

D10A in Cas9

Promoter

CMV

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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mBaoJin-JIP4

Plasmid

#244978

Purpose

Express JIP4 in mammalian cells with mBaoJin tag (monomeric StayGold)

Depositor

Luis Bonet-Ponce

Article

Bonet-Ponce et al J Cell Biol. 2025 Nov 3;224(11):e2024040

Insert

JIP4 (SPAG9 Human)

Tags

mBaoJin

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pSmBtA3-d7F

Plasmid

#245784

Purpose

Expression of mutated bovine arrestin-3 (deleted residues 175, 177, 178, 182-185) tagged with SmBiT and Flag in mammalian cells

Depositor

Vsevolod Gurevich

Article

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

arrestin-3 (ARR3 Bovine)

Tags

Flag and SmBiT

Expression

Mammalian

Mutation

deleted residues 175, 177, 178, 182-185

Promoter

CMV

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-RILPL1 (R293A)

Plasmid

#244981

Purpose

Express RILPL1 in mammalian cells with mCherry tag expressing a R293A mutation

Depositor

Luis Bonet-Ponce

Article

Bonet-Ponce et al J Cell Biol. 2025 Nov 3;224(11):e2024040

Insert

RILPL1 (RILPL1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

R293A

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1 mouse NT

Plasmid

#242421

Purpose

Expression of a non-targeting shRNA in mouse cells

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

Non-targeting shRNA

Expression

Mammalian

Promoter

U6

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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