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pVRb17_up1691

Plasmid

#49711

Depositor

Christopher Voigt

Publication

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

sfgfp

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pecf17_up1691

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only

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KHBD00130

Plasmid

#34284

Depositor

Bart Deplancke

Publication

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG3935 (al Fly)

Use

Gateway Cloning

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pDONR223-PSKH1

Plasmid

#23514

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PSKH1 (PSKH1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pFredB-PTTG1

Plasmid

#16585

Depositor

Bert Vogelstein

Publication

Jallepalli et al Cell. 2001 May 18. 105(4):445-57.

Insert

PTTG1 (PTTG1 Human)

Expression

Mammalian

Mutation

3' targeting element

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pSKB3.TM1468

Plasmid

#11431

Depositor

Sung-Hou Kim

Publication

Schulze-Gahmen et al Proteins. 2003 Mar 1. 50(4):526-30.

Insert

fatty acid binding protein

Expression

Bacterial

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

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pSKB3.MPN314

Plasmid

#11433

Depositor

Sung-Hou Kim

Publication

Chen et al Proteins. 2004 Jun 1. 55(4):785-91.

Insert

cell division protein

Expression

Bacterial

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pLIC.B2.MPN330

Plasmid

#11536

Depositor

Sung-Hou Kim

Publication

Lemesle-Varloot et al J Steroid Biochem Mol Biol. 1992 Mar . 4

Insert

FAD synthetase

Tags

HisTag, non-cleavable

Expression

Bacterial

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pAB042a

Plasmid

#79192

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Publication

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Plac62 gIII, luxAB; Ptet rpoZ-YSX1

Expression

Bacterial

Promoter

Plac62; Ptet

Availability

Academic Institutions and Nonprofits only

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pAB061i10

Plasmid

#79227

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Publication

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Ppro1 rpoZ-HA4 (Y87R); PlacIq lacI

Expression

Bacterial

Promoter

Ppro1; PlacIq

Availability

Academic Institutions and Nonprofits only

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pAB061i11

Plasmid

#79228

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Publication

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Ppro1 rpoZ-HA4 (Y87K); PlacIq lacI

Expression

Bacterial

Promoter

Ppro1; PlacIq

Availability

Academic Institutions and Nonprofits only

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pAB061i8

Plasmid

#79231

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Publication

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Ppro1 rpoZ-HA4 (Y87W); PlacIq lacI

Expression

Bacterial

Promoter

Ppro1; PlacIq

Availability

Academic Institutions and Nonprofits only

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pAB061i9

Plasmid

#79232

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Publication

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Ppro1 rpoZ-HA4 (Y87F); PlacIq lacI

Expression

Bacterial

Promoter

Ppro1; PlacIq

Availability

Academic Institutions and Nonprofits only

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Nb24-His6 (b-arrestin1 nanobody)

Plasmid

#259692

Purpose

E. coli periplasmic expression of the active-state-specific beta-arrestin1 (arrestin2) nanobody Nb24 with a C-terminal His6 tag

Depositor

Laura Wingler

Publication

Elgeti et al J Biol Chem. 2026 Feb;302(2):111117. doi

Insert

Nb24-His6

Tags

His6

Expression

Bacterial

Promoter

plac

Available since

Aug. 17, 2026

Availability

Academic Institutions and Nonprofits only

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pDONR221-SARS-CoV-2-nsp5

Plasmid

#247689

Purpose

Gateway cloning compatible entry vector for SARS-CoV-2 nsp5.

Depositor

Sho Iketani

Publication

Chithelen et al Nat Commun. 2026 May 19;17(1):6575. doi:

Insert

SARS-CoV-2 3CL protease

Use

Synthetic Biology

Available since

July 20, 2026

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(6AW)RS

Plasmid

#207619

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Aza-L-Tryptophan (6AW) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Publication

Abdelkader et al Angew Chem Int Ed Engl. 2026 Jul 13:e899

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available since

July 16, 2026

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(4AW)RS

Plasmid

#207617

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 4-Aza-L-Tryptophan (4AW) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Publication

Abdelkader et al Angew Chem Int Ed Engl. 2026 Jul 13:e899

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available since

July 16, 2026

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(5AW)RS

Plasmid

#207618

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 5-Aza-L-Tryptophan (5AW) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Publication

Abdelkader et al Angew Chem Int Ed Engl. 2026 Jul 13:e899

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available since

July 16, 2026

Availability

Academic Institutions and Nonprofits only

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OlexA_mini35S_AvrPtoB_SYFP2_pENTR_D-TOPO

Plasmid

#257233

Purpose

A cloning backbone designed for the insertion of GOI (genes of interest) to be C-terminal SYFP2 tagged

Depositor

On Sun Lau

Publication

Kou et al New Phytol. 2026 Apr;250(2):1330-1347. d

Insert

OlexA_mini35S_AvrPtoB_SYFP2

Use

Gateway Cloning

Tags

SYFP2

Promoter

OlexA_mini35S

Available since

July 14, 2026

Availability

Academic Institutions and Nonprofits only

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PCW57.1-cyto-GshF-GFP

Plasmid

#257608

Purpose

Doxycycline-inducible expression of cytosolic GshF-GFP

Depositor

Kivanc Birsoy

Publication

Liu et al Nat Cell Biol. 2026 Apr 17. doi: 10.1038

Insert

cyto-GshF

Expression

Mammalian

Promoter

tight TRE promoter

Available since

July 8, 2026

Availability

Academic Institutions and Nonprofits only

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pDMJ2_SARS1_Urbani-SARS2_WuHu1_CTD2

Plasmid

#257103

Purpose

Expreses SARS-CoV-1 Urbani Spike fused with SARS-CoV-2 WuhanHu-1 Spike CTD2

Depositor

Jeremy Luban

Publication

Yurkovetskiy et al bioRxiv [Preprint]. 2023 Apr 24:2023.03.

Insert

SARS-CoV-1 Spike

Expression

Mammalian

Mutation

Ins 575-908

Available since

June 29, 2026

Availability

Academic Institutions and Nonprofits only

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