We narrowed to 24,434 results for: lars;

1. pcDNA3 ER-Ras-LOCKR-PL Key

   Plasmid

   #216506

   Purpose

   Ras activity dependent proximity labeler, Key only, ER localized

   Depositor

   David Baker

   Article

   Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

   Insert

   ER-Ras-LOCKR-PL Key
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3 Mito-Ras-LOCKR-PL Key

   Plasmid

   #216507

   Purpose

   Ras activity dependent proximity labeler, Key only, mitochondria localized

   Depositor

   David Baker

   Article

   Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

   Insert

   Mito-Ras-LOCKR-PL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mCherry-Vector

   Plasmid

   #214477

   Purpose

   Vector control for mCherry fusions. Created from the backbone of Addgene #55043 mCherry-Ezrin-N-14 from Michael Davidson.

   Depositor

   Natalie Ahn

   Article

   Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. P1K-HFL1-8flag-LEU

   Plasmid

   #207035

   Purpose

   Expression of Hfl1-8Flag. Uses auxotrophic marker LEU2(Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   HFL1
   

   Use

   Tags

   8xFLAG

   Expression

   Yeast

   Mutation

   Promoter

   pHFL1

   Available since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET SUMO-FL-syt7

   Plasmid

   #213630

   Purpose

   Bacterial expression of SUMO-tagged mouse FL-syt7

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   FL-syt7 (Syt7 Mouse)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PCUP1-4V5-Pho8-Met

   Plasmid

   #207029

   Purpose

   Expression of 4V5-Pho8 under CPU1 promoter.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   Pho8
   

   Use

   Tags

   4xV5

   Expression

   Yeast

   Mutation

   Promoter

   pCUP1

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. BS-Trp-PCUP1-4myc-Pho8

   Plasmid

   #207028

   Purpose

   Expression of 4Myc-Pho8 under CPU1 promoter.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   Pho8
   

   Use

   Tags

   4xMyc

   Expression

   Yeast

   Mutation

   Promoter

   pCUP1

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PCUP1-Vac8-GFP-Ura

   Plasmid

   #207027

   Purpose

   Expression of Vac8-GFP under CPU1 promoter.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   Vac8
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   pCUP1

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PCUP1-GFP-Vma13-Ura

   Plasmid

   #207026

   Purpose

   Expression of GFP-Vma13 under CPU1 promoter.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   Vma13 ORF
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   pCUP1

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. P1K-3HA-Atg8∆GR-MET15

    Plasmid

    #207033

    Purpose

    Expression of 3HA-Atg8∆GR. This variant cannot be conjugated to PE.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    ATG8
    

    Use

    Tags

    3xHA

    Expression

    Yeast

    Mutation

    ∆GR

    Promoter

    pATG8

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PCUP1-mRuby3-Pho8-406

    Plasmid

    #207043

    Purpose

    Expression of mRuby3-Pho8 under CPU1 promoter.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    PHO8
    

    Use

    Tags

    mRuby3

    Expression

    Yeast

    Mutation

    Promoter

    pCUP1

    Available since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 3'-BirA*-FLAG minimal CMV pCDNA5

    Plasmid

    #212047

    Purpose

    LR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locus

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. β2AR-PPI-CALPon1.0 (APEX)

    Plasmid

    #213406

    Purpose

    Peroxidase (APEX) reporter for beta 2-adrenergic receptor (β2AR) and β-arrestin 2 interaction (component 1)

    Depositor

    Wenjing Wang

    Article

    Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

    Insert

    β2AR-E5-TEVcs-APEX(201-250)-K5-EGFP-APEX(2-200)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Ca-LiPPI-CLAPon1.0 (TEVp)

    Plasmid

    #213409

    Purpose

    Light-gated peroxidase (APEX) reporter for detecting calcium-dependent CaM and MK2 interaction (component 2)

    Depositor

    Wenjing Wang

    Article

    Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

    Insert

    CaM-V5-TEVp
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYSD063

    Plasmid

    #212916

    Purpose

    Encodes mRuby2 fluorescent protein as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    mRuby2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 5'-3xFLAG minimal CMV pCDNA5

    Plasmid

    #212044

    Purpose

    LR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locus

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 5'-BirA*-FLAG minimal CMV pCDNA5

    Plasmid

    #212046

    Purpose

    LR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locus

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 5'-3xFLAG GFP minimal CMV pCDNA5

    Plasmid

    #212086

    Purpose

    LR vector for integration of GFP into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUC57-ITR2-H1-GFP sgRNA Merry Target 3- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid

    #210743

    Purpose

    Coding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 3

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    SaSp Cas9
    Merry sgRNA GFP Target 3
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    N-terminal Sa Cas9, C-terminal Sp Cas9

    Promoter

    H1 and eCMV

    Available since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC57-ITR2-H1-GFP sgRNA Target 3- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2

    Plasmid

    #210739

    Purpose

    Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 3

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    Sp Cas9 (cas9 S pyogenes)
    Sp sgRNA GFP Target 3
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    Promoter

    H1 and eCMV

    Available since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge