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pet16b-Avi-ubiquitin_rbs_BirA

Plasmid

#134897

Purpose

Expresses HIS-Avi-tagged ubiquitin for recombinant protein production

Depositor

Andrew Deans

Publication

Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

Inserts

Ubiquitin (UBB Human)
BirA

Tags

10HIS-AviTag-PreScission(3C)protease

Expression

Bacterial

Promoter

RBS and T7

Available since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET-28c(+)-YTHDF2

Plasmid

#102275

Purpose

For bacterial expression of His-tagged human YTHDF2

Depositor

Samie Jaffrey

Publication

Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

Insert

YTHDF2 (YTHDF2 Human)

Tags

6X HIS

Expression

Bacterial

Promoter

T7

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pFB1_hMSH2

Plasmid

#129423

Purpose

Expression human MSH2 protein using baculovirus

Depositor

Peggy Hsieh

Publication

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Insert

human MSH2 (MSH2 Human)

Expression

Insect

Promoter

Polyhedrin promoter

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKM402

Plasmid

#107770

Purpose

Expression of phage Che9c RecT for oligo-mediated recombineering in mycobacteria

Depositor

Kenan Murphy

Publication

Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

Inserts

Che9C RecT
TetR (tet repressor)
cat
kan

Expression

Bacterial

Promoter

Cat promoter, Ptet, kan promoter, and pTB21

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pmCherry-Zdk1-EB1C

Plasmid

#107695

Purpose

C-terminal half of π-EB1, mCherry-tagged, dissociates from microtubule ends in blue light

Depositor

Torsten Wittmann

Publication

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE1 (MAPRE1 Human)

Tags

Zdk1 and mCherry

Expression

Mammalian

Mutation

EB1 aa 186-268

Promoter

CMV

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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YEp195-CUP1-His-Ubi

Plasmid

#99540

Purpose

Yeast episomal vector for expression of His-tagged ubiquitin; URA3 marker

Depositor

Helle Ulrich

Publication

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

UBI3(1-76) (RPL40A Budding Yeast)

Tags

7xHis

Expression

Yeast

Promoter

CUP1

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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CMV10-3xFlag Skp2 delta-F

Plasmid

#81116

Purpose

mammalian expression of F-box deleted Skp2

Depositor

Sung Hee Baek

Publication

Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

Insert

SKP2 (SKP2 Human)

Tags

3xFlag

Expression

Mammalian

Mutation

deleted F box

Promoter

CMV

Available since

Sept. 24, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
MSCV PIG C/EBPa

Plasmid

#66985

Purpose

Retroviral expression plasmid for mouse C/EBPa expression; containing bicistronic IRES-GFP

Depositor

Christopher Vakoc

Publication

Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

Insert

C/EBP alpha (Cebpa Mouse)

Use

Retroviral

Promoter

LTR

Available since

July 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CAG:: ChR2HA-2a-hM4D

Plasmid

#52520

Purpose

Co-expresses HA tagged channelrhodopsin and hM4D (Gi)

Depositor

Scott Sternson

Publication

Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

Insert

ChR2-2a-hM4D

Tags

2xHA on ChR2

Expression

Mammalian

Promoter

CAG

Available since

May 9, 2014

Availability

Academic Institutions and Nonprofits only

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pLJM60-S6K1(wt) barcoded

Plasmid

#48801

Purpose

expresses wt S6K1

Depositor

David Sabatini

Publication

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

S6K1 (Rps6kb1 Rat)

Use

Lentiviral

Tags

FLAG and barcode: GGATCC

Expression

Mammalian

Promoter

CMV

Available since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only
pAC5-dual-dCas9VP48-sgTetO

Plasmid

#48237

Purpose

Dual expression construct expressing both dCas9VP48 and sgTetO from separate promoters

Depositor

Rudolf Jaenisch

Publication

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9VP48 and sgTetO

Use

CRISPR

Tags

HA-tag and VP48

Expression

Mammalian

Mutation

D10A H840A (catalytically inactive)

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) mMff isoform 5

Plasmid

#44601

Depositor

David Chan

Publication

Loson et al Mol Biol Cell. 2013 Jan 2.

Insert

mMff isoform 5 (Mff Mouse)

Expression

Mammalian

Available since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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FLAG-EglN2-pLenti6

Plasmid

#36950

Depositor

William Kaelin

Publication

Koivunen et al Nature. 2012 Feb 15;483(7390):484-8. doi

Insert

EglN2 (EGLN2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Wildtype

Promoter

CMV

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGEX 4T1 ERbeta

Plasmid

#35563

Depositor

Harish Srinivas

Publication

Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

Insert

Estrogen Receptor beta (ESR2 Human)

Tags

GST

Expression

Bacterial

Available since

March 26, 2012

Availability

Academic Institutions and Nonprofits only

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p3HA-hMIB2

Plasmid

#33312

Depositor

Vanessa Redecke

Publication

Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

Insert

MIB2 (MIB2 Human)

Tags

3xHA

Expression

Mammalian

Promoter

CMV

Available since

March 26, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pcDNA6.MCV.cLT206.V5(CM2B4)

Plasmid

#28189

Depositor

Patrick Moore

Insert

MCPyV Large T antigen (206 wild-type strain)

Tags

V5

Expression

Bacterial and Mammalian

Available since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pEGFP-Bax1-2/L-6

Plasmid

#30533

Depositor

Richard Youle

Publication

Edlich et al Cell. 2011 Apr 1. 145(1):104-16.

Insert

BCL2-associated X protein (BAX Human)

Tags

eGFP

Expression

Mammalian

Mutation

F30C E44C C62S L63C C126S P130C

Available since

July 28, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pcDNA3.1 AKT3 HA (CT-gfp)

Plasmid

#27293

Depositor

Morris Birnbaum

Insert

protein kinase B gamma (Akt3 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Y473F

Available since

April 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pRS-puro shNEDD4L #1

Plasmid

#27016

Depositor

Joan Massague

Publication

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

shRNA NEDD4L #1 (NEDD4L Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAL190

Plasmid

#22279

Purpose

Expresses Tim DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment

Depositor

Christopher Voigt

Publication

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

TimDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)

Use

Synthetic Biology

Expression

Mammalian

Promoter

SV40

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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