We narrowed to 88,932 results for: Mal

1. pdCas9-DNMT3A-PuroR

   Plasmid

   #71667

   Purpose

   Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

   Depositor

   Vlatka Zoldos

   Article

   Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

   Insert

   S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Human, Synthetic, S. pyogenes)
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and T2A-PuroR

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9

   Promoter

   CBh

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAS_4xhyb PylT A41AA C55A CRFR1 95TAA 263TAG-HA

   Plasmid

   #154780

   Purpose

   plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and rat CRFR1 95TAA 263TAG, for transient transfection or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   CRFR1 (Crhr1 Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   95TAA 263TAG in CRFR1-HA, hybrid PylT with A41AA …

   Promoter

   EF1

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q60

   Plasmid

   #111749

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q60 (polyQ repeat)

   Promoter

   CMV and P10

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTAHR TH-p2a-TD:Tomato (floxed selection Puro)

   Plasmid

   #135814

   Purpose

   Fluorescent reporter for knock-in of TdTomato into the TH locus

   Depositor

   Tim Ahfeldt, Lee Rubin

   Article

   Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

   Insert

   TH-p2a-TD:Tomato (red fluorophore protein) construct as a reporter for TH expression. (TH Human)
   

   Tags

   p2a-TdTomato

   Expression

   Mammalian

   Available Since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. PX458-3xHA-en31FnCas9

   Plasmid

   #201949

   Purpose

   Mammalian expression plasmid of enhanced activity FnCas9 variant en31FnCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-en31FnCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   G1243T, E1369R, E1603H on FnCas9

   Promoter

   Cbh

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. HDM-SARS2-Spike-delta21

   Plasmid

   #155130

   Purpose

   Encodes SARS-CoV-2 Spike with 21 aa C-terminal deletions for lentiviral pseudotyping

   Depositor

   Jesse Bloom

   Article

   Crawford et al Viruses. 2020 May 6;12(5). pii: v1205051

   Insert

   SARS-CoV-2 Spike-delta21 (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Codon optimized to H. sapiens using IDT codon opt…

   Promoter

   CMV

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q23

   Plasmid

   #111726

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q23 (polyQ repeat)

   Promoter

   CMV and P10

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK5_mEGFP-CRTC1-MAML2

   Plasmid

   #237638

   Purpose

   For overexpression of mEGFP-CRTC1-MAML2

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   CRTC1-MAML2 (CRTC1, MAML2 Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.3-Nm-dCas9-p300 Core

   Plasmid

   #61365

   Purpose

   encodes N. meningiditis dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   Nm-dCas9-p300 Core (EP300 Human, Neisseria meningiditis, Synthetic)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   HA tag and NLS

   Expression

   Mammalian

   Mutation

   D16A, D587A, H588A, N611A in Nm Cas9

   Promoter

   CMV

   Available Since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q19

    Plasmid

    #111733

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q19 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q15

    Plasmid

    #111732

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q15 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHA TST 3C SMO EABR

    Plasmid

    #234989

    Purpose

    For production of Extracellular Vesicles (EVs), with the receptor Smoothened (Smo), Twin-Strep-tag (TST), and an HA tag at its N-terminus on their surface

    Depositor

    Monica Gonzalez-Magaldi, Daniel Leahy

    Article

    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

    Insert

    SMO-EABR (SMO Human)
    

    Tags

    HA, Twin-Strep-Tag, HRV 3C site

    Expression

    Mammalian

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pdCas9-DNMT3A-PuroR_v2

    Plasmid

    #74407

    Purpose

    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR (v2) for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR_v2 (DNMT3A Human, Synthetic, S. pyogenes)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    CBh

    Available Since

    April 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-N1_c-SRC

    Plasmid

    #171850

    Purpose

    pEGFP-N1_c-SRC

    Depositor

    Michele Pagano

    Article

    Moro et al Nat Cell Biol. 2020 Sep;22(9):1130-1142.

    Insert

    c-SRC (SRC Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Added a linker sequence in 3' of c-SRC: GSGS

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q78

    Plasmid

    #111737

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q78 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Spike Display_6P-D614G

    Plasmid

    #172734

    Purpose

    Expression vector for SARS-CoV-2 (HexaPro-D614G) spike used in the Spike Display system

    Depositor

    IIya Finkelstein

    Article

    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

    Insert

    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

    Expression

    Mammalian

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    CMV

    Available Since

    Aug. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. PX458-3xHA-en1FnCas9

    Plasmid

    #201947

    Purpose

    Mammalian expression plasmid of enhanced activity FnCas9 variant en1FnCas9 with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    3xHA-NLS-en1FnCas9-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    E1369R on FnCas9

    Promoter

    Cbh

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFUSE-rIgG-Fc2-ICAM1

    Plasmid

    #156462

    Purpose

    Expresses a rabbit Fc-ICAM1 fusion protein in mammalian cells

    Depositor

    Dirk Mielenz

    Article

    Reimer et al Cell Rep. 2020 Aug 11;32(6):108030. doi:

    Insert

    Intercellular Adhesion Molecule 1 (Icam1 Mouse)
    

    Tags

    ICAM1-rFc

    Expression

    Bacterial and Mammalian

    Mutation

    endogenous signal peptide replaced with IL2 signa…

    Promoter

    hEF1-HTLC

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q23

    Plasmid

    #111723

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q23 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lenti-CMV-PE2-IRES-ZeoR

    Plasmid

    #207352

    Purpose

    Lentiviral transfer plasmid containing CMV-driven expression cassette encoding PE2 enzyme for prime editing.

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    Prime editor 2 (PE2) enzyme
    

    Use

    Lentiviral

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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