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pENTR-Arl13b1

Plasmid

#40870

Depositor

Tamara Caspary

Article

Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

Insert

Arl13b (Arl13b Mouse)

Use

Gateway entry

Available Since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only
pETCON-TNF

Plasmid

#67748

Purpose

Tumor Necrosis Factor Alpha Yeast Display Plasmid

Depositor

Timothy Whitehead

Article

Kowalsky et al J Biol Chem. 2015 Oct 30;290(44):26457-7

Insert

TNF-Alpha

Expression

Bacterial and Yeast

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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PGL3-MK17

Plasmid

#21486

Depositor

Shoumo Bhattacharya

Article

Leung et al Genomics. 1999 Nov 1. 61(3):307-13.

Insert

Cited2 Promoter (CITED2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

July 23, 2009

Availability

Academic Institutions and Nonprofits only
px330 RAD21 gRNA

Plasmid

#165592

Purpose

Insertion of RAD21 degron

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

RAD21 (Rad21 Mouse)

Expression

Mammalian

Mutation

NA

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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E12 2/5-pCS2

Plasmid

#20913

Depositor

Stephen Tapscott

Article

Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

Insert

E12 2/5 (TCF3 Human)

Expression

Mammalian

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pSR-puro-Exo84

Plasmid

#31116

Depositor

Christopher Counter

Article

Issaq et al Mol Cancer Res. 2010 Feb . 8(2):223-31.

Insert

Exo84 shRNA (EXOC8 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 20, 2011

Availability

Academic Institutions and Nonprofits only
pMJ915v2+Nterm Spe1 +Cterm Age1 site

Plasmid

#88914

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-mFzd10-1D4

Plasmid

#42272

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 10 (Fzd10 Mouse)

Tags

1D4

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pMOD_A0908

Plasmid

#91027

Purpose

Module A, Promoter: EC1.2, Gene: TREX2-P2A-AtCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TREX2-P2A-AtCas9

Use

CRISPR

Promoter

EC1.2

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pSicoR mouse Dicer3

Plasmid

#14781

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

Dicer shRNA (Dicer1 Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only
AIRAP 10: PSMD14.TEV.GST.V1.pCDNA3

Plasmid

#21800

Depositor

David Ron

Article

Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

Insert

PSMD14 (Psmd14 Mouse)

Tags

GST and TEV

Expression

Mammalian

Available Since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-RaACR_687-EYFP

Plasmid

#111077

Purpose

Anion channelrhodopsin from Rhodomonas abbreviata for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

Insert

Anion channelrhodopsin RaACR_687

Tags

EYFP

Expression

Mammalian

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pME-nlsGal80(opt)

Plasmid

#51618

Purpose

vertebrate expression of codon-optimized Gal80 with N-terminal NLS and no stop

Depositor

Joshua Bonkowsky

Article

Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

Insert

Gal80(opt) (GAL80 Budding Yeast)

Use

Vertebrate expression

Tags

NLS

Mutation

codon optimized

Available Since

May 19, 2014

Availability

Academic Institutions and Nonprofits only

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pRU51

Plasmid

#167677

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUB4-2::spCas9

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pNLRP11major.MYC

Plasmid

#131196

Purpose

Mammalian expression of human NLRP11 188S

Depositor

Thomas Kufer

Article

Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

Insert

NLRP11 188S (NLRP11 Human)

Tags

myc

Expression

Mammalian

Mutation

188S

Promoter

pCMV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-SacB-Cas9-T2A-mCherry_(BbsI)

Plasmid

#117070

Purpose

Encodes Cas9, T2A-mCherry, and AmpR which creates a CRISPR system application with reliable positive selection

Depositor

Kim Failor

Inserts

Cas9
SacB

Use

CRISPR

Tags

3x FLAG, NLS, and T2A-mCherry

Expression

Mammalian

Promoter

CBh and U6

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pME-puro-CLIP-FLAG-CD59

Plasmid

#50375

Purpose

Express CLIP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

Depositor

Reika Watanabe

Article

Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

Insert

CLIP

Expression

Mammalian

Promoter

SR alpha

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pFA-LEU2-I-SceI

Plasmid

#67595

Purpose

I-SceI expression regulated by tetracycline inducible system in S. pombe

Depositor

Kurt Runge

Article

Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

Inserts

nmt terminator
I-SceI
TetR

Use

Gene targeting in yeast

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-nDsRedFL-miRNT

Plasmid

#177808

Purpose

A nontargeting miRNA based on the pre-miR- 30a structure (Qiu et al., 2008) has been inserted into the EcoRV of pCAG-nDsRedFL-intron.

Depositor

Rikako Sanuki

Article

Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

Insert

miR-NT

Expression

Mammalian

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAC3-ISH

Plasmid

#15854

Depositor

Peter Mombaerts

Article

Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

Insert

Adenylate Cyclase 3 (Adcy3 Mouse)

Expression

Bacterial

Mutation

contains residues 787-1021

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only