We narrowed to 18,078 results for: comp

1. PuroR-P2A-DD-TCOF1-donor

   Plasmid

   #60747

   Purpose

   Donor plasmid for HR-mediated recombination to insert puromycin resistance and append a destabilization domain to the N-terminus of TCOF1

   Depositor

   Benhur Lee

   Article

   Park et al PLoS One. 2014 Apr 17;9(4):e95101. doi:

   Insert

   TCOF1 (TCOF1 Human)
   

   Use

   Targeting donor

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pAAV-Ef1a-Coff/Fon-BFP

   Plasmid

   #137131

   Purpose

   Intersectional viral expression of BFP in cells expressing Flp AND NOT Cre

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-BFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   n/a

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACT1:Cas9-GFP, U6:sgUPRT

   Plasmid

   #122853

   Purpose

   Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum UPRT gene

   Depositor

   David Sibley

   Article

   Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

   Inserts

   Cas9-GFP
   U6-sgUPRT
   

   Use

   CRISPR; Cas9-gfp plasmid for genome editing of cr…

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   Cryptosporidium parvum U6 and Cryptosporidium par…

   Available Since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEBTetBl-CLIP-CPAP

   Plasmid

   #136864

   Purpose

   Mammalian expression of the centrosomal protein CENPJ N-terminally fused to CLIP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   CLIP-CENPJ (CENPJ Synthetic, Human)
   

   Use

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. BlastR-P2A-DD-TCOF1-donor

   Plasmid

   #60748

   Purpose

   Donor plasmid for HR-mediated recombination to insert blasticidin resistance and append a destabilization domain to the N-terminus of TCOF1

   Depositor

   Benhur Lee

   Article

   Park et al PLoS One. 2014 Apr 17;9(4):e95101. doi:

   Insert

   TCOF1 (TCOF1 Human)
   

   Use

   Targeting donor

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pLV-puro-N-Flag-h-SNRNP40

   Plasmid

   #134254

   Purpose

   Lentivector encoding Flag-tagged human SNRNP40

   Depositor

   Bruce Beutler

   Article

   Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

   Insert

   SNRP40 (SNRNP40 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   May 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTT3-SP-6xHis-PVR(FL)-FLAG

   Plasmid

   #157622

   Purpose

   Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   PVR (PVR Human)
   

   Use

   Tags

   6xHis and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR-HLA-A0201-His

   Plasmid

   #108213

   Purpose

   HLA-A0201 his tagged

   Depositor

   Sébastien Walchli

   Article

   Lampen et al Immunohorizons. 2018 Jul 2;2(6):172-184.

   Insert

   major histocompatibility complex, class I, B (HLA-A Human)
   

   Use

   Entry vector for gateway system

   Tags

   his

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223-ZNF574

   Plasmid

   #88644

   Purpose

   Donor Vector containing ZNF574 transcription factor, part of the Human TFome Collection

   Depositor

   Marc Vidal

   Article

   Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

   Insert

   ZNF574 (ZNF574 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Last nucleotide of stop codon removed to allow fo…

   Promoter

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRGEN-CMV-10XGCN4-L1-dCjCas9 S-D

    Plasmid

    #169912

    Purpose

    Expression of a SunTag-dCjCas9 fusion protein in which 10 copies of the GCN4 peptide are fused to the N terminus of dCjCas9.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Insert

    dCjCas9
    

    Use

    CRISPR

    Tags

    10XGCN4, 2xSV40NLS, and SV40NLS-HA

    Expression

    Mammalian

    Mutation

    D8A, H559A in cjCas9

    Promoter

    CMV

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-Ef1a-Con/Fon-BFP

    Plasmid

    #137129

    Purpose

    Intersectional viral expression of BFP in cells expressing both Cre AND Flp

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-BFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCOLADuet-1_nsp10 (SARS-CoV-2)

    Plasmid

    #169158

    Purpose

    To express SARS-CoV-2 nsp10 in E. coli

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    nsp10 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Methionine

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T7

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. p663-UBC-miniTurbo-V5-NRF2

    Plasmid

    #207811

    Purpose

    N-terminal tagged NRF2 for miniTurbo proximity experiments

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    miniTurbo-V5-NRF2 (NFE2L2 Human)
    

    Use

    Lentiviral

    Tags

    miniTurbo-V5

    Expression

    Mutation

    Promoter

    UBC

    Available Since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. p667-UBC-NRF2-V5-miniTurbo

    Plasmid

    #207812

    Purpose

    C-terminal tagged NRF2 for miniTurbo proximity experiments

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    NRF2-V5-miniTurbo (NFE2L2 Human)
    

    Use

    Lentiviral

    Tags

    V5-miniTurbo

    Expression

    Mutation

    Promoter

    UBC

    Available Since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAVS1-TetOn-3XFLAG-renilla-beta-globin-PTC39-AAVS1

    Plasmid

    #184398

    Purpose

    Donor plasmid for stable integration of Renilla luciferase NMD(+) PTC39 reporter at AAVS1

    Depositor

    Robert Bradley

    Article

    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

    Insert

    Renilla luciferase beta-globin-PTC39
    

    Use

    CRISPR; Donor plasmid for hdr

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    PTC in beta-globin at amino acid position 39

    Promoter

    Tet-On 3G

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJT410 AAV-Rapamycin Inducible-NFZ-Citrine with bGH pA

    Plasmid

    #202059

    Purpose

    Inducible AAV activator

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    AAV-Rapamycin Inducible-NFZ-Citrine with bGH pA
    

    Use

    AAV; Aav with reporter

    Tags

    Expression

    Mammalian

    Mutation

    ITRs intact

    Promoter

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pD649-HAsp-ROBO4-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156864

    Purpose

    Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    ROBO4 (ROBO4 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX459-CTNNB1-S45

    Plasmid

    #164587

    Purpose

    Expresses a gRNA that overlaps the S45 codon of human CTNNB1

    Depositor

    Renee van Amerongen

    Article

    de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

    Insert

    CTNNB1 gRNA (CTNNB1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCW57.1_SPTLC1_C133W

    Plasmid

    #181921

    Purpose

    3rd generation, Inducible lentiviral plasmid for expression of human mutant SPTLC1 C133W

    Depositor

    Christian Metallo

    Article

    Cordes et al J Lipid Res. 2022 Sep 14:100281. doi: 10

    Insert

    Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1) mutant C133W (SPTLC1 Human)
    

    Use

    Lentiviral; Mammalian expression, , doxycycline i…

    Tags

    Expression

    Mammalian

    Mutation

    cysteine at position 133 of the coding region of …

    Promoter

    tight TRE promoter

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. BcLOV4 fusion tools screening plasmid #2

    Plasmid

    #174512

    Purpose

    Cloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP in a pcDNA3.1 backbone.

    Depositor

    Brian Chow

    Article

    Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

    Insert

    plasmid B [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP
    

    Use

    Tags

    3xFLAG and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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