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pMRMTK-clo41

Plasmid

#204016

Purpose

Cloning plasmid containing the BFP insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Blue fluorescent protein

Expression

Plant

Promoter

N/A

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo42

Plasmid

#204017

Purpose

Cloning plasmid containing the YFP insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Yellow fluorescent protein

Expression

Plant

Promoter

N/A

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo11

Plasmid

#203981

Purpose

Cloning plasmid containing the Lambda tl3 terminator insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Lambda tl3 Terminator

Expression

Plant

Promoter

N/A

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo13

Plasmid

#203983

Purpose

Cloning plasmid containing the tonB & SoxR terminators insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

tonB & SoxR Terminator

Expression

Plant

Promoter

N/A

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo15

Plasmid

#203985

Purpose

Cloning plasmid containing the Elu ES2 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Elu ES2

Expression

Plant

Promoter

Elu ES2

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo26

Plasmid

#204000

Purpose

Cloning plasmid containing the Bpi ES1 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Bpi ES1

Expression

Plant

Promoter

Bpi ES1

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo28

Plasmid

#204002

Purpose

Cloning plasmid containing the Bpi ES3 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Bpi ES3

Expression

Plant

Promoter

Bpi ES3

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo1

Plasmid

#203919

Purpose

Cloning plasmid containing the BHR1 origin insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

BHR1 Origin of Replication

Expression

Plant

Promoter

N/A

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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TBPL1 KO 1

Plasmid

#200210

Purpose

gRNA for TBPL1 KO

Depositor

Sheila Teves

Article

Kwan et al Elife. 2023 Mar 30;12:e83810. doi: 10.75

Insert

TBP1 KO 1 (Tbpl1 Mouse)

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pET PI-SceI C-His K403A

Plasmid

#195612

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K403A active site mutation

Depositor

Frederick Gimble

Article

Gimble et al J Biol Chem. 1998 Nov 13;273(46):30524-9

Insert

PI-SceI

Expression

Bacterial

Mutation

A1463G, A1464C

Promoter

T7

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pSLiP-G1G2

Plasmid

#188965

Purpose

L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown

Depositor

Seung-Goo Lee

Article

Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

Inserts

dCas9
sgRNA 1: atcggtcgcattgttttccactagg, sgRNA 2: gttagacgctgattacatggactagg

Use

Synthetic Biology

Expression

Bacterial

Promoter

PrhaBAD

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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mU6-sgCebpa_v2-hu6-sgCebpb_v2

Plasmid

#177252

Purpose

Expresses Cebpa_v2 (mU6), Cebpb_v2(hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpa_v2/sgCebpb_v2

Use

Lentiviral

Promoter

mU6/hU6

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pGMC00003

Plasmid

#166720

Purpose

sgRNA against mouse Kitl

Depositor

Raj Chari

Article

Rice et al Nat Commun. 2018 Nov 30;9(1):5099. doi:

Insert

Kitl (Kitl Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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gh167

Plasmid

#106826

Purpose

expression of gRNA targeting A4GALT

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

A4GALT (A4GALT Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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p426_Cas9_gRNA-ARS1206a

Plasmid

#87398

Purpose

All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1206a sequence CGAACATTTTTCCATGCGCT in yeast chromosome 12.

Depositor

Aindrila Mukhopadhyay

Article

Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

Insert

Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1206a

Use

CRISPR

Promoter

ADH1, pTyrosine

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pUC19 gene 60 thru gap

Plasmid

#49160

Purpose

Template for generating gene 60 mRNA truncated two codons after coding gap

Depositor

Nils Walter

Article

Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

Insert

Bacteriophage T4 gene 60

Mutation

gene 60 mRNA truncated two codons after coding ga…

Promoter

T7

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pGLx2-set1-H1017K

Plasmid

#53258

Purpose

GAL driven LexA-tagged SET1-catalytically dead (H1017K)

Depositor

Jean Cook

Article

Rizzardi et al Genetics. 2012 Oct;192(2):371-84. doi: 1

Insert

SET1

Tags

LexA

Expression

Yeast

Mutation

H1017K mutation - catalytically dead

Promoter

GAL1/10

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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pYSD704

Plasmid

#253013

Purpose

Encodes Trichoderma reesei Hydrophobin1 (HFB1) as a Type 3b' part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Article

Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102

Insert

Hydrophobin1 (HFB1)

Expression

Bacterial

Available Since

May 7, 2026

Availability

Academic Institutions and Nonprofits only

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pET24-His6-Hatag-mCherry

Plasmid

#253054

Purpose

Encodes His6 HA mCherry in pET24 plasmid for bacterial expression with a T7 promoter

Depositor

Paul Young

Article

Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102

Insert

mCherry

Expression

Bacterial

Available Since

May 5, 2026

Availability

Academic Institutions and Nonprofits only

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pET24-His6-GFP1-10

Plasmid

#253053

Purpose

Encodes 6xHistidine-tagged GFP1-10 fluorescence complementation fragment in a modified pET24d vector backbone for expression in E. coli

Depositor

Paul Young

Article

Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102

Insert

GFP

Expression

Bacterial

Available Since

May 5, 2026

Availability

Academic Institutions and Nonprofits only

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