We narrowed to 18,502 results for: multi
-
Plasmid#140956PurposeFor PHB production in E. coli (T7-pAC). Please note that this is listed as #140955 in the publication.DepositorInsertphaCAB
ExpressionBacterialAvailable SinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGNW6
Plasmid#122093Purposesuicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cellsDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBR_attB(bxb)_ccdB_lox_v2
Plasmid#198040PurposeBxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)DepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyExpressionMammalianAvailable SinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCFD4d-U6-1:white1-U6-3:white1
Plasmid#84005PurposepCFD4d expresses white sgRNA-1 from a U6:3 promoter and the same white sgRNA-1 from a U6:1 promoterDepositorInsertswhite sgRNA-1
white sgRNA-1
UseCRISPRPromoterU6:1, U6:3, and dU6-1; dU6-3Available SinceJan. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TRE-dTomato-GCaMP6s
Plasmid#99864Purposeexpresses dTomato and GCaMP6s under control of the Tet-off systemDepositorInsertdTomato, GCaMP6s
PromoterTet-responsive promoterAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6-Universal
Plasmid#52694PurposeThis plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.DepositorInsertsCas9
Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
UseCRISPR; Toxoplasma gondiiTags3X FLAG and Nuclear localization signalPromoterTgTUB1 and Toxoplasma U6Available SinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTiger-OptoSOS
Plasmid#86439PurposeSingle-construct blue light optogenetic Ras activator for use in DrosophilaDepositorInsertOptoSOS
TagstagRFPExpressionInsectPromoterGal4-UASAvailable SinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX459-homo_PTGS1-1
Plasmid#184731PurposeCOX1-KO in HeLaDepositorInsertA gRNA targeting the human COX1 gene.
UseCRISPRAvailable SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX459-homo_PTGS1-2
Plasmid#184732PurposeCOX1-KO in HeLaDepositorInsertA gRNA targeting the human COX1 gene.
UseCRISPRAvailable SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX459-homo_PTGS2-3
Plasmid#184733PurposeCOX2-KO in HeLaDepositorInsertA gRNA targeting the human COX2 gene.
UseCRISPRAvailable SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX459-homo_PTGS2-4
Plasmid#184734PurposeCOX2-KO in HeLaDepositorInsertA gRNA targeting the human COX2 gene.
UseCRISPRAvailable SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCas9CyMutL
Plasmid#166981PurposeEncodes aTc-inducible spCas9 and cumate-inducible MutLE32K for transient suppression of mismatch repairDepositorInsertsUseCRISPR and Synthetic BiologyExpressionBacterialMutationE32KPromoterpCymRC and pLacIAvailable SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMH48
Plasmid#131861PurposeProduces holo-Cph1* in E. coliDepositorInsertsCph1(1-514,Y263F)-RGD-His6-Cys
HO1
PcyA
ExpressionBacterialPromoterT7 promoter-1 and T7 promoter-2Available SinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT05
Plasmid#223377PurposeT-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.DepositorInsertZmUbi-SpCas9-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAUX_OL
Plasmid#166246Purposeuse as an auxiliary temperature-sensitive plasmid to successfully transform pdCas9_CL plasmidDepositorInsert[P112-sgRNA-term]-[J23116_B34-dCas9-B15]
ExpressionBacterialPromoterEc-TTL-P112 and BBa_J23116Available SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-LMO-IRES-EGFP
Plasmid#178309PurposeExpress the bacterial lactate 2-monooxygenase (LMO) enzyme in mammalian cellsDepositorInsertLactate 2-monooxygenase
TagsEGFPExpressionMammalianPromoterCMVAvailable SinceJan. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomKate2-CaURA3
Plasmid#44878PurposeYeast tagging vector to create gene fusion with yeast optimized mKate2 with CaUra3 selectionDepositorInsertyomKate2
ExpressionYeastAvailable SinceNov. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti-dCas9-KRAB-gRNA-TRE-blast
Plasmid#201152PurposeLentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.DepositorInsertsdCas9-KRAB
gRNA-TRE
UseCRISPR and LentiviralTagsHAMutationgRNA sequence: TACGTTCTCTATCACTGATAvailable SinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGTag Hygro EGFP nLAP -2
Plasmid#194322PurposeCRISPR/Cas9-mediated generic protein taggingDepositorInsertHygro-P2A-nLAP(EGFP)
UseBacterial cloning vectorAvailable SinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGTag Hygro EGFP nLAP -1
Plasmid#194321PurposeCRISPR/Cas9-mediated generic protein taggingDepositorInsertHygro-P2A-nLAP(EGFP)
UseBacterial cloning vectorAvailable SinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only