We narrowed to 12,188 results for: LEA
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Plasmid#8874DepositorInsertMLL min A.D. (KMT2A Human)
TagsGSTExpressionBacterialMutationF2852Y: ILPSDIMDFVL --> ILPSDIMDYVLAvailable sinceAug. 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
3703 pGEX4T-3 MLL AD V2853A
Plasmid#8875DepositorInsertMLL min A.D. (KMT2A Human)
TagsGSTExpressionBacterialMutationV2853A: ILPSDIMDFVL --> ILPSDIMDFALAvailable sinceAug. 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-DEN2-NS3(S171-K618)
Plasmid#258837PurposeExpression of His-tagged Dengue Virus Type 2 (DEN2) Helicase NS3 Catalytic Domain (S171 – K618) in E. coliDepositorInsertHelicase NS3 (Catalytic Domain) (POLY Dengue Virus Type 2)
TagsKLAAALEHHHHHH and MGRGSHHHHHHGMAExpressionBacterialMutationDeleted Amino Acids 1-170PromoterT7 lacAvailable sinceAug. 11, 2026AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-GP-TFIIB-2xStrep
Plasmid#213919PurposeSleeping beauty vector for expression of C-terminally strep-tagged TFIIBDepositorInsertTFIIB-2xStrep (GTF2B Human)
Tags2xStrepExpressionMammalianMutationCoding region changed for resistance to Dharmaco…PromoterEF-1EF1a/RPBSAAvailable sinceJune 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pAvA139
Plasmid#248145Purposemutated LuxR transcriptional activator for expression in yeast: fused with Gal4 activation domain with 'gen1' mutationsDepositorInsertluxR (gen1 mutations)
UseIntegration vectorTagsGal4 activation domain and nuclear localization s…ExpressionYeastMutationGal4_AD: N24K, P41 (CCA→CCG), N46D, T92S, V98 (GT…PromoterpPGK1Available sinceJan. 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
pLV-mEGFP-hCTNNA1_DM1-3_H0-ABD+
Plasmid#234554PurposeCTNNA1 M-domain deletion mutant with enhanced actin-binding domain mutationsDepositorAvailable sinceOct. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eVRQR-P2A-EGFP (KAC1028)
Plasmid#242663PurposeCMV promoter expression plasmid for human codon optimized SpCas9-eVRQR(S55R)-BPNLS(SV40)-3xFLAG-P2A-EGFPDepositorInsertpCMV-SpCas9-eVRQR(S55R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
UseCRISPRTagsBPNLSExpressionMammalianMutationeVRQR mutations in SpCas9(S55R/D1135V/G1218R/R133…PromoterCMV and T7Available sinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
Mirror GFP[ENE(WT)-mascRNA(mut 8356-8370)](pAVA3874)
Plasmid#239353PurposeExpresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of MALAT1 ENE(WT)-mascRNA(mut 8356-8370) reporter.DepositorInsertMALAT1 ENE(WT)-mascRNA(mut 8356-8370)
ExpressionMammalianMutationMALAT1 ENE(WT)-mascRNA(mut 8356-8370: ctacgaccacc…Available sinceAug. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR-B_2xsgRNAs_UPF3A/UPF3B (pAVA3129)
Plasmid#239329PurposeLentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting UPF3A and UPF3BDepositorInsertU6-driven sgRNA1 targting UPF3A and 7SK-driven sgRNA2 targeting UPF3B (UPF3B Human)
UseCRISPR and LentiviralAvailable sinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
E. coli Lig-N-HiBiT
Plasmid#215437PurposeBacterially expressed E. coli Lig-N-HiBiT for Ni-NTA purificationDepositorInsertNAD-dependent DNA ligase LigA [Escherichia coli], N-HiBiT, N-10XHis.Thrombin
Tags10xHis-tag with thrombin site and HiBiT TagExpressionBacterialMutationCodon Optimized for bacterial expression, T7 gene…PromoterT7 PromotorAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
E. coli Lig-C-HiBiT
Plasmid#215438PurposeBacterially expressed E. coli Lig-C-HiBiT for Ni-NTA purificationDepositorInsertNAD-dependent DNA ligase LigA [Escherichia coli], C-HiBiT, C-10XHis.Thrombin
Tags10xHis-tag with thrombin site and HiBiT TagExpressionBacterialMutationCodon Optimized for bacterial expression, T7 gene…PromoterT7 PromotorAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST (E44A L47A D49A A50K Q51A; deltaWIPI2)
Plasmid#223777PurposeExpression of recombinant protein for purificationDepositorInsertBNIP3 soluble part (BNIP3 Human)
TagsThrombin cleavage-GSTExpressionInsectMutationE44A L47A D49A A50K Q51A; disrupts WIPI2 bindingAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST (I224A/L227A/W276A/I279A; deltaLIR1+3)
Plasmid#223754PurposeExpression of recombinant protein for purificationDepositorInsertBCL2L13 soluble part (BCL2L13 Human)
TagsThrombin cleavage-GSTExpressionBacterialMutationexpresses aa 1-465, with I224A/L227A/W276A/I279A;…Available sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST (W276A/I279A/I307A/V310A; deltaLIR1+4)
Plasmid#223755PurposeExpression of recombinant protein for purificationDepositorInsertBCL2L13 soluble part (BCL2L13 Human)
TagsThrombin cleavage-GSTExpressionBacterialMutationexpresses aa 1-465, with W276A/I279A/I307A/V310A;…Available sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST (Y213A/I216A/W276A/I279A; deltaLIR1+2)
Plasmid#223752PurposeExpression of recombinant protein for purificationDepositorInsertBCL2L13 soluble part (BCL2L13 Human)
TagsThrombin cleavage-GSTExpressionBacterialMutationexpresses aa 1-465, with Y213A/I216A/W276A/I279A;…Available sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_NIX(1-182aa)-THROMBIN-GST (E72A/L75A/D77A/E81A; deltaWIPI2)
Plasmid#223753PurposeExpression of recombinant protein for purificationDepositorInsertNIX (BNIP3L) soluble part (BNIP3L Human)
TagsThrombin cleavage-GSTExpressionBacterialMutationexpresses aa 1-182, with E72A/L75A/D77A/E81A; del…Available sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 ILVtoED solvvol
Plasmid#232958PurposeGalactose iduced expression of Gcn4 LVtoED solvvol in yeastDepositorInsertGcn4 ILVtoED solvvol
TagsTEV cleavage siteExpressionYeastMutationS101T, D103E, S104T, D115E, N116Q, S117T, S122T, …PromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Twin-strep-TEV-DGKδ2-DSAMD
Plasmid#223721PurposeExpress human diacylglycerol kinase delta 2- SAM domain deletion mutant (N-terminal TEV protease cleavable Twin-Strep-fusion protein) in mammalian cellDepositorInsertDGKD (DGKD Human)
TagsTEV cleavable site and Twin-Strep-tagExpressionMammalianMutationdeleted sterile alpha motif domain (deleted amino…PromoterCAG and chicken β-actin promoterAvailable sinceJan. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H322
Plasmid#228246PurposemT2Del_EPACdDEPCD_Q270E_L777A_K778A_tdcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertmT2Del_EPACdDEPCD_Q270E_L777A_K778A_tdcp173Venus(ST) (RAPGEF3 Human)
ExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-NTOM20-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-bArr2-TevCs-P2A-EGFP
Plasmid#210505Purposeexpresses NTOM20-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-bArr2-TevCs-P2A-EGFP component in mammalian cellsDepositorInsertNTOM20-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-bArr2-TevCs-P2A-EGFP
ExpressionMammalianPromoterCMVAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only