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We narrowed to 62,955 results for: cloning

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Showing: 11921 - 11940 of 62955 results

  6. pJC.15A.sgRNA.TA

    Plasmid
    #232483
    Purpose
    sgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection.
    Depositor
    James Collins
    Publication
    Chua et al Microbiol Resour Announc. 2025 Apr 28:e0
    Inserts
    Toxin-antitoxin System from CD630
    medium-copy-number p15A origin of replication
    mCherry
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    P3 constitutive promoter and native promoter
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    232483-plasmid-map-sequence-id-466415

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  11. pGJJ055

    Plasmid
    #183622
    Purpose
    ddPCA mutagenesis plasmid - Efficient mutant library cloning plasmid suitable for oligo synthesis, error prone PCR or nicking mutagenesis strategies.
    Depositor
    Ben Lehner
    Publication
    Faure et al Nature. 2022 Apr;604(7904):175-183. doi:
    Type
    Empty backbone
    Expression
    Bacterial
    Available since
    May 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    183622-plasmid-map-sequence-id-361477

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  12. PROM_MpUBE2

    Plasmid
    #136109
    Purpose
    PROM for cloning with 5UTR_MpUBE2. PROM+5UTR_MpUBE2 has ubiquitous expression in early satges of gemmae development.
    Depositor
    Jim Haseloff
    Publication
    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102
    Insert
    PROM_MpUBE2 Mapoly0106s0019
    Use
    Synthetic Biology
    Expression
    Plant
    Mutation
    BsaI/ SapI domesticated
    Available since
    Nov. 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    136109-plasmid-map-sequence-id-275127

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  13. 5UTR_MpUBE2

    Plasmid
    #136110
    Purpose
    5UTR for cloning with PROM_MpUEB2. PROM+5UTR_MpUBE2 has ubiquitous expression in early stages of gemmae development.
    Depositor
    Jim Haseloff
    Publication
    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102
    Insert
    5UTR_MpUBE2 Mapoly0106s0019
    Use
    Synthetic Biology
    Expression
    Plant
    Mutation
    BsaI/ SapI domesticated
    Available since
    Nov. 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    136110-plasmid-map-sequence-id-275128

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  14. pSHDY*in_PpetE:BCD2:mVenus

    Plasmid
    #133970
    Purpose
    Heterologous, copper-inducible expression of the reporter fluorophor mVenus in Synechocystis sp. PCC 6803. Used as backbone for cloning of GOI downstream of the PpetE:BCD2 expression module.
    Depositor
    Pia Lindberg
    Publication
    Dienst et al Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.
    Insert
    mVenus
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    inserted gene is codon optimized for Synechocyst…
    Promoter
    PpetE:BCD2
    Available since
    April 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133970-plasmid-map-sequence-id-267750

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Showing: 11921 - 11940 of 62955 results