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V1-MESA-45F-M-dCas9

Plasmid

#84504

Purpose

MESA target chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, M cleavage sequence, and dCas9-VP64

Depositor

Joshua Leonard

Article

Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

Insert

V2-MESA-35F-M-dCas9

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pHD-mW-attPCC-FRT (pTL717)

Plasmid

#115158

Purpose

Vector for generating dsDNA donors for HDR. Contains an attP-CC docking site, the visible GMR-white for identifying backbone incorporation flanked by FRT sites for FLP-mediated removal.

Depositor

Tobias Langenhan

Article

Blanco-Redondo et al G3 (Bethesda). 2018 Jul 31. pii: g3.118.

Insert

mW attP-CC FRT

Expression

Insect

Available Since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Vinculin-23

Plasmid

#55453

Purpose

Localization: Focal Adhesions, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Vinculin (VCL Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
p416GAL1-Zipp.-L-IFP-F[1] (Yeast)

Plasmid

#52895

Purpose

Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[1] by the linker (GGGGS) X 2. Zipper-L-IFP-F[1] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

Zipp.-L-IFP-F[1]

Tags

L-IFP-F[1]

Expression

Yeast

Promoter

GAL1

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pSBbi-TIR1/Pur

Plasmid

#171682

Purpose

constitutive expression of TIR1-myc in sleeping beauty transposon vector with selection marker (puromycin)

Depositor

Randy Poon

Article

Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

Insert

OsTIR1-MYC

Tags

MYC

Expression

Mammalian

Promoter

EF-1a promoter

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCMB-TGN61r

Plasmid

#61175

Purpose

Fluorescent reporter for trans-Golgi network mCherry-MtSYP61 expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtSYP61

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pMOD_C3013

Plasmid

#91100

Purpose

Module C, Promoter: PvUbi2, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP (+ BaeI site for GT donor cloning)

Use

Unspecified

Promoter

PvUbi2

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-alphaCaMKII-E2-Crimson-3XNLS-pA

Plasmid

#183803

Purpose

AAV vector to express nuclear localized E2-Crimson from CaMKIIa promoter

Depositor

Jonathan Ploski

Article

de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

Insert

E2-Crimson-NLS

Use

AAV

Tags

NLS

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-scrCdh2.1 GFP

Plasmid

#209100

Purpose

GFP expressing scramble of shRNA targeting Cdh2

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

scrCdh2.1

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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141_pT3TS_iCre

Plasmid

#171793

Purpose

Plasmid for optimised iCre RNA synthesis using T3 promoter.

Depositor

Jean Giacomotto

Article

Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

Insert

optimised Cre for RNA expression

Expression

Bacterial

Promoter

T3

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-RNF17S

Plasmid

#35414

Depositor

Jeremy Wang

Article

Pan et al Development. 2005 Sep;132(18):4029-39. E

Insert

Rnf17s (Rnf17 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2013

Availability

Academic Institutions and Nonprofits only
pNRB143

Plasmid

#36452

Depositor

Douglas Bishop

Article

Bishop et al Genes Cells. 1999 Aug;4(8):425-44.

Insert

RAD54 (RAD54 Budding Yeast)

Expression

Yeast

Promoter

T7

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only
JEH127

Plasmid

#179299

Purpose

Expresses dCas9 fused to VPR

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

Insert

dCas9-VPR

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive Cas9)

Promoter

CAG

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pNM3749

Plasmid

#154108

Purpose

loxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Inserts

LoxP-SEC-LoxP
FRT::rpl-28p::GFP::his-58::FRT3

Use

Cre/Lox; Rmce

Expression

Worm

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti GFP-AMOT p130 Y242/287A

Plasmid

#32829

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

Insert

AMOT p130 (AMOT Human)

Use

Lentiviral

Tags

GFP-AMOT

Expression

Mammalian

Mutation

Y242/287A

Available Since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only
pFL-V5-GW

Plasmid

#78857

Purpose

DULIP Prey Destination Vector (Gateway, N-terminal)

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Type

Empty backbone

Use

Gateway Cloning

Tags

Firefly luciferase, V5-tag

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pPA-RL-GW

Plasmid

#78855

Purpose

DULIP Bait Destination Vector (Gateway, N-terminal)

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Type

Empty backbone

Use

Gateway Cloning

Tags

Protein A-Renilla luciferase

Expression

Mammalian

Promoter

CMV

Available Since

July 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCFJ1117 - [4-1] - Promoter - Psmu-1(no_atg)

Plasmid

#159886

Purpose

Three-fragment Gateway compatible promoter with PATCs for germline expression from arrays

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[4-1] - Promoter - Psmu-1(no_atg)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMDJ80 - pENTR[4-1] - Promoter - Psmu-2 (no_start)

Plasmid

#159887

Purpose

Three-fragment Gateway compatible promoter with PATCs for germline expression from arrays

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[4-1] - Promoter - Psmu-2 (no_start)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-MKRN1 WT

Plasmid

#78766

Purpose

To detect localization of MKRN1 in live mammalian cells

Depositor

Jaewhan Song

Article

Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

Insert

MKRN1 (MKRN1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only