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pcDNA-h-mmPtch1-B-Y233C-HA

Plasmid

#120904

Purpose

Expresses Ptch1-B (Y233C) for function assays

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291, Y…

Promoter

CMV

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-L234C-HA

Plasmid

#120905

Purpose

Expresses Ptch1-B (L234C)

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291, L…

Promoter

CMV

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-Sfxn2 (CG6812)

Plasmid

#110643

Purpose

Retroviral expression of Flag-tagged Drosophila CG6812 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

CG6812 (CG6812 Fly)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

codon-optimized cDNA (no amino acid mutations)

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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CDk4-luciferase fragment B

Plasmid

#86659

Purpose

Luciferase reporter containing CDK4 promoter

Depositor

Jaime Modiano

Article

Baksh et al Mol Cell. 2002 Nov;10(5):1071-81.

Insert

CDK4 promoter (CDK4 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

generated by cleavage at the KpnI site from 880 (…

Promoter

CDK7

Available Since

June 20, 2017

Availability

Academic Institutions and Nonprofits only

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COL4A3BP gRNA (BRDN0001148588)

Plasmid

#77848

Purpose

3rd generation lentiviral gRNA plasmid targeting human COL4A3BP

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

COL4A3BP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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ADRBK2 gRNA (BRDN0001145704)

Plasmid

#77946

Purpose

3rd generation lentiviral gRNA plasmid targeting human ADRBK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ADRBK2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ADRBK2 gRNA (BRDN0001146920)

Plasmid

#77944

Purpose

3rd generation lentiviral gRNA plasmid targeting human ADRBK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ADRBK2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ADRBK2 gRNA (BRDN0001147892)

Plasmid

#77945

Purpose

3rd generation lentiviral gRNA plasmid targeting human ADRBK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ADRBK2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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COL4A3BP gRNA (BRDN0001147254)

Plasmid

#77850

Purpose

3rd generation lentiviral gRNA plasmid targeting human COL4A3BP

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

COL4A3BP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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COL4A3BP gRNA (BRDN0001146451)

Plasmid

#77849

Purpose

3rd generation lentiviral gRNA plasmid targeting human COL4A3BP

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

COL4A3BP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge