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EGFP-hPMCA4b delta6

Plasmid

#47590

Depositor

Emanuel Strehler

Article

Padanyi et al Biochim Biophys Acta. 2009 Jun;1793(6):1

Insert

PMCA4bdelta6 (ATP2B4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

6 C-terminal AA deleted (needed for PDZ binding)

Promoter

CMV

Available Since

Aug. 26, 2013

Availability

Academic Institutions and Nonprofits only
pSB819-PKR-hum-FYS

Plasmid

#20046

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

Protein kinase R (EIF2AK2 Human)

Expression

Yeast

Mutation

L394F, F489Y

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pSB819-PKR-hum-FYA

Plasmid

#20049

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

Protein kinase R (EIF2AK2 Human)

Expression

Yeast

Mutation

L394F, F489Y, S492A

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pSB819-PKR-hum-LYA

Plasmid

#20050

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

Protein kinase R (EIF2AK2 Human)

Expression

Yeast

Mutation

F489Y, S492A

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pX459-Plxna1 gRNA

Plasmid

#246574

Purpose

CRISPR vector co-expressing Cas9 and a mouse Plxna1 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Plxna1 gRNA (Plxna1 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-asp-2-flag-tev-halo-his

Plasmid

#227153

Purpose

For T-REX experiments of asp-2.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

asp-2 (asp-2 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCI_IZUMO1-V5_GFPnes

Plasmid

#240497

Purpose

Cell fusion assays

Depositor

Benjamin Podbilewicz

Article

Brukman et al J Cell Biol. 2023 Feb 6;222(2):e20220714

Insert

IZUMO1 (Izumo1 Mouse)

Tags

GFPnes and V5

Expression

Mammalian

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCI_IZUMO1-V5_H2B-RFP

Plasmid

#240496

Purpose

Cell fusion assays

Depositor

Benjamin Podbilewicz

Article

Brukman et al J Cell Biol. 2023 Feb 6;222(2):e20220714

Insert

IZUMO1 (Izumo1 Mouse)

Tags

H2B-RFP and V5

Expression

Mammalian

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCI_JUNO_H2B-RFP

Plasmid

#240498

Purpose

Cell fusion assays

Depositor

Benjamin Podbilewicz

Article

Brukman et al J Cell Biol. 2023 Feb 6;222(2):e20220714

Insert

JUNO (Izumo1r Mouse)

Tags

H2B-RFP and V5

Expression

Mammalian

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCDF_AaRaf1/BSD2

Plasmid

#229516

Purpose

Expresses Anthoceros agrestis chaperones: Raf1,BSD2

Depositor

Laura Gunn

Article

Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

Inserts

Rubisco accumulation factor 1
BSD2

Expression

Bacterial

Mutation

N terminus chloroplast transit peptide truncation

Promoter

T7

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-NFHΔ36-72

Plasmid

#232060

Purpose

Expresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 36-72

Depositor

Sanjay Kumar

Article

Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

Insert

Neurofilament-Heavy tail domain lacking residues 36-72

Tags

6xHis

Expression

Bacterial

Mutation

added N-terminal tryptophan and tyrosine, and cys…

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-NFHΔ462-638

Plasmid

#232061

Purpose

Expresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 462-638

Depositor

Sanjay Kumar

Article

Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

Insert

Neurofilament-Heavy tail domain lacking residues 462-638

Tags

6xHis

Expression

Bacterial

Mutation

added N-terminal tryptophan and tyrosine, and cys…

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-NFMtail

Plasmid

#232056

Purpose

Expresses construct for surface tethering: R. norvegicus NFM tail domain

Depositor

Sanjay Kumar

Article

Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

Insert

Neurofilament-Medium tail domain

Tags

6xHis, thrombin

Expression

Bacterial

Mutation

cDNA codon-optimized for E. coli; added N-termina…

Promoter

T7

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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mproSAAS (21 kDa)

Plasmid

#231634

Purpose

Mammalian expression of the N-terminal domain of the mouse chaperone protein proSAAS-(1-180)

Depositor

Iris Lindberg

Article

Fortenberry et al J Biol Chem. 2002 Feb 15;277(7):5175-86.

Insert

Pcsk1N (Pcsk1n Mouse)

Expression

Mammalian

Mutation

Deletion of C-terminus (aa181-225)

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-rsGreenF-E-Geminin(1-110)-IRES-Dronpa-Cdt(30-120)

Plasmid

#205761

Purpose

Single-color FUCCI4 sensor for G1/S cell cycle transition and M/G1 transition

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

rsGreenF-E-Geminin(1-110)-IRES-Dronpa-Cdt(30-120)

Use

Lentiviral

Expression

Mammalian

Mutation

wt

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTBL2620 MTK3-TetR

Plasmid

#226670

Purpose

MTK3 part containing the coding sequence of TetR

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d

Insert

TetR

Use

Synthetic Biology

Promoter

none

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL2621 MTK3-LacI

Plasmid

#226671

Purpose

MTK3 part containing the coding sequence of LacI

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d

Insert

LacI

Use

Synthetic Biology

Promoter

none

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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RT-5

Plasmid

#220442

Purpose

Construct used to generate the PUF3/PUP2/3HA tagging strains. (aka pBSII/PUF3 5’ flank/PUP2-DADA/3HA/URA3/PUF3 3’ flank)

Depositor

Marvin Wickens

Article

Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

Insert

PUF3 (puf-3 Nematode)

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-Myc-Caspase-11_humanized-mutant

Plasmid

#214316

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (Casp4 Mouse)

Use

Retroviral

Tags

Myc

Mutation

KLS288YKT_KASIHS348TPRAKA

Promoter

MESV

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-Myc-Caspase-4_R269D

Plasmid

#214310

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (CASP4 Human)

Use

Retroviral

Tags

Myc

Mutation

R269D

Promoter

MESV

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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