We narrowed to 128 results for: spycatcher

1. pET11b-SpyCatcher-M2e

   Plasmid

   #165990

   Purpose

   SpyCatcher-fused influenza M2 ectodomain with removable HisTag

   Depositor

   Vesa Hytönen

   Article

   Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

   Insert

   SpyCatcher-M2e
   

   Use

   Tags

   HisTag and SpyCatcher

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCG-D-mSpyCatcher-His6-E

   Plasmid

   #87376

   Purpose

   An ORF part-containing vector harbouring the minimal SpyCatcher (ΔN1ΔC22) sequence with a His6 tag at its C-terminus and 4 bp overhangs specific for the ‘downstream SpyPart’ position within an ORF ass

   Depositor

   Tom Ellis

   Article

   Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

   Insert

   mSpyCatcher
   

   Use

   Synthetic Biology

   Tags

   His6 tag

   Expression

   Mutation

   Promoter

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIBA3-SpyCatcherEQ-sfGFP

   Plasmid

   #107421

   Purpose

   For production of inactive SpyCatcherEQ protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.

   Depositor

   Jack Leo

   Article

   Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/

   Insert

   SpyCatcher
   

   Use

   Tags

   His tag, StrepII, and superfolder GFP

   Expression

   Bacterial

   Mutation

   mutation of glutamate 77 to glutamine

   Promoter

   Tet

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-SpyCatcher -AVI

   Plasmid

   #223635

   Purpose

   Protein purification of SpyCatcher003 (S49C) with AVI and 6xHis tags from E. coli

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   SpyCatcher003 S49C
   

   Use

   Tags

   AVI tag and His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)_SpyCatcher-6aa-sfCherry2(1-10)

   Plasmid

   #117484

   Purpose

   Expresses tandem binder (SpyCatcher002-6aa-sfCherry2(1-10)) in mammalian cells

   Depositor

   Bo Huang

   Article

   Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

   Insert

   SpyCatcher002-6aa-sfCherry2(1-10)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP-Talin head-SpyCatcher003

   Plasmid

   #133566

   Purpose

   Expresses EGFP-Talin head-SpyCatcher003 in mammalian cells

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   EGFP-Talin head-SpyCatcher003
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST14-SpyCatcher003-TEVs-SpyTag003DA

   Plasmid

   #216283

   Purpose

   Expresses SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow TEV protease to remove SpyTag003DA and thereby uncage SpyCatcher003 reactivity.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   SpyCatcher003-TEVs-SpyTag003DA
   

   Use

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQE80L-SpyCatcher-ELP-GFP

   Plasmid

   #69835

   Purpose

   Bacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do

   Insert

   SpyCatcher-ELP-GFP
   

   Use

   Tags

   6x His Tag, GFP, and TEV Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T5 promoter/lac operator element

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-SIN-BX SpyCatcher003-hCD52

   Plasmid

   #210565

   Purpose

   Expresses Spycatcher003 on the cell surface using human CD52 hinge

   Depositor

   Omer Dushek

   Article

   Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

   Insert

   Spycatcher003 fused to human CD52 hinge
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET22b 6His SpyCatcher-GRFT

    Plasmid

    #204502

    Purpose

    Bacterial expression of GRFT with N-terminal SpyCatcher fusion and GS linker

    Depositor

    Urartu Seker

    Article

    Ozkul et al Adv Mater Interfaces. 2022 Sep 13:220112

    Insert

    GRFT
    

    Use

    Synthetic Biology

    Tags

    6xHis and SpyCatcher

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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