We narrowed to 17,068 results for: EMB

1. pEntry_SARS-CoV-1-Membrane

   Plasmid

   #168840

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Pascal Falter-Braun

   Article

   Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

   Insert

   SARS-CoV-1-Membrane (M )
   

   Use

   Gateway-compatible entry vector

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. membrane-mCherry-SpVasa-C3

   Plasmid

   #185587

   Purpose

   to recruit Vasa-C3 to membrane

   Depositor

   Mamiko Yajima

   Article

   Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

   Insert

   Vasa-C3
   

   Use

   In vitro transcription (mrna synthesis)

   Tags

   mCherry and membrane targeting sequence from Neur…

   Mutation

   C3 mutant

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. membrane-mCherry-SpVasa

   Plasmid

   #185586

   Purpose

   to recruit Vasa to membrane

   Depositor

   Mamiko Yajima

   Article

   Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

   Insert

   Vasa
   

   Use

   In vitro transcription (mrna synthesis)

   Tags

   mCherry, S-Tag and membrane targeting sequence fr…

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. membrane-HsPLK1-dead

   Plasmid

   #185572

   Purpose

   to see protein dynamics

   Depositor

   Mamiko Yajima

   Article

   Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

   Insert

   PLK1-dead (PLK1 Human)
   

   Use

   In vitro transcription (mrna synthesis)

   Tags

   membrane targeting sequence from Neuromodulin (ML…

   Mutation

   K82R kinase-dead mutant

   Available Since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Plasma membrane-targeted optoPLD

   Plasmid

   #140114

   Purpose

   Encodes CRY2-mCherry-PLD-P2A-CIBN-CAAX for mammalian expression

   Depositor

   Jeremy Baskin

   Article

   Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Phospholipase D
   

   Tags

   CAAX, CIBN, CRY2, P2A, and mCherry

   Expression

   Mammalian

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Plasma membrane-targeted optodPLD

   Plasmid

   #140061

   Purpose

   Encodes CRY2-mCherry-dPLD-P2A-CIBN-CAAX for mammalian expression

   Depositor

   Jeremy Baskin

   Article

   Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Phospholipase D
   

   Tags

   CAAX, CIBN, CRY2, P2A, and mCherry

   Expression

   Mammalian

   Mutation

   H170A (catalytic dead)

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSP64-membrane-mCherry-LOV

   Plasmid

   #135599

   Purpose

   Expression of membrane-mCherry-LOV mRNA for microinjection for use of optogenetics in S. purpuratus

   Depositor

   Mamiko Yajima

   Article

   Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

   Insert

   membrane-mCherry-LOV
   

   Use

   In vitro transcription (mrna synthesis)

   Tags

   LOV and mCherry

   Promoter

   Unknown

   Available Since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. cASIC1 concatemer - fully assembled

   Plasmid

   #233133

   Purpose

   To create cASIC1 channels of defined stoichiometry in mammalian cells

   Depositor

   David MacLean

   Article

   Purohit et al Biophys J. 2024 Oct 15;123(20):3507-3518

   Insert

   ASIC1 (ASIC1 Chicken)
   

   Expression

   Mammalian

   Available Since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. PhOTO-N (pMTB:memb-Cerulean-2A-H2B-Dendra2)

   Plasmid

   #92400

   Purpose

   Constitutive expression of nuclear green-to-red photoconvertible Dendra2 and the membrane blue fluorescent protein Cerulean.

   Depositor

   Periklis Pantazis

   Article

   Dempsey et al PLoS One. 2012;7(3):e32888. doi: 10.1371

   Inserts

   Dendra2
   Cerulean
   

   Use

   Zebrafish expression

   Tags

   H2B tag and membrane tag

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPW3756 : CMVd3-ERmembrane-HaloTag-KKMP

    Plasmid

    #185678

    Purpose

    Low-level transient expression of a single HaloTag protein with a C-terminal KKMP ER retention signal, targeted to the ER membrane.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERmembrane-HaloTag-KKMP
    

    Expression

    Mammalian

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. PhOTO-M (pMTB:memb-Dendra2-2A-H2B-Cerulean)

    Plasmid

    #92401

    Purpose

    Constitutive expression of membrane green-to-red photoconvertible Dendra2 and the nuclear blue fluorescent protein Cerulean.

    Depositor

    Periklis Pantazis

    Article

    Dempsey et al PLoS One. 2012;7(3):e32888. doi: 10.1371

    Inserts

    Dendra2
    Cerulean
    

    Use

    Zebrafish expression

    Tags

    H2B tag and membrane tag

    Promoter

    bactin2

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Nano-lantern-plasma membrane/pcDNA3

    Plasmid

    #51975

    Purpose

    mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to the plasma membrane

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern
    

    Tags

    HIS and Lyn

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pPW3757 : CMVd3-ERmembrane-2xHaloTag-KKMP

    Plasmid

    #185679

    Purpose

    Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERmembrane-2xHaloTag-KKMP
    

    Expression

    Mammalian

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. HA-MAO-N Mitochondrial Membrane-Targeting Cloning Vector

    Plasmid

    #139015

    Purpose

    To express a protein of interest fused to the N-terminus of HA-MAO for mitochondrial outer membrane targeting

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Tags

    HA-MAO

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPW3781 : CMVd3-ERmembrane-GST-HaloTag-KKMP

    Plasmid

    #185686

    Purpose

    Low-level transient expression of a HaloTag protein targeted to the ER membrane, fused with a GST for constitutive dimerization.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERmembrane-GST-HaloTag-KKMP
    

    Expression

    Mammalian

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVX-EF1a-EGFP-Membrin-IRES-Puromycin

    Plasmid

    #134865

    Purpose

    Expresses EGFP-tagged Golgi apparatus marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Membrin (GOSR2 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMOS010: CheRiff-eGFP channelrhodopsin (plasma membrane)

    Plasmid

    #163044

    Purpose

    CheRiff-eGFP channelrhodopsin (plasma membrane)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    CheRiff
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPW3783 : CMVd3-ERmembrane-GST-2xHaloTag-KKMP

    Plasmid

    #185687

    Purpose

    Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane, fused with a GST for constitutive dimerization (making a HaloTag tetramer).

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERmembrane-GST-2xHaloTag-KKMP
    

    Expression

    Mammalian

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK5 FLAG membrane-bound lipin-1 (TMD)

    Plasmid

    #120277

    Purpose

    Expression of ER/Golgi-tethered lipin-1 in mammalian cells

    Depositor

    Sirio Dupont

    Article

    Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. d

    Insert

    Lipin-1 (Lpin1 Mouse)
    

    Tags

    FLAG and in-frame fusion with the TMD Golgi-local…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pD649-HAsp-EMB-COMP5AP-AviTag-9xHis

    Plasmid

    #157244

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    EMB (EMB Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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