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Plasmid#156958PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertPTPRK (PTPRK Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-PTPRU-Fc(DAPA)-AviTag-6xHis
Plasmid#156960PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertPTPRU (PTPRU Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-PTPRT-Fc(DAPA)-AviTag-6xHis
Plasmid#156954PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertPTPRT (PTPRT Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-PTPRM-Fc(DAPA)-AviTag-6xHis
Plasmid#156955PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertPTPRM (PTPRM Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-PTPRQ(Trunc1)-COMP5AP-AviTag-9xHis
Plasmid#157614PurposeMammalian expression of cell-surface protein partial extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertPTPRQ (PTPRQ Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-PTPRQ(Trunc3)-COMP5AP-AviTag-9xHis
Plasmid#157574PurposeMammalian expression of cell-surface protein partial extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertPTPRQ (PTPRQ Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-PTPRQ(Trunc2)-COMP5AP-AviTag-9xHis
Plasmid#157575PurposeMammalian expression of cell-surface protein partial extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertPTPRQ (PTPRQ Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-PTPRQ(Trunc1)-Fc(DAPA)-AviTag-6xHis
Plasmid#157050PurposeMammalian expression of cell-surface protein partial extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertPTPRQ (PTPRQ Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-PTPRQ(Trunc3)-Fc(DAPA)-AviTag-6xHis
Plasmid#157010PurposeMammalian expression of cell-surface protein partial extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertPTPRQ (PTPRQ Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-PTPRQ(Trunc2)-Fc(DAPA)-AviTag-6xHis
Plasmid#157011PurposeMammalian expression of cell-surface protein partial extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertPTPRQ (PTPRQ Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CTE-Luc
Plasmid#60881PurposeReporter construct to study unspliced RNA export. Coding sequence of luciferase gene was sandwiched between 5' and 3' splice site sequences originating from the HIV gag/pol gene followed by CTEDepositorInsertLuc-CTE
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmMTOR_AA
Plasmid#148202PurposeInsect Expression of DmMTORDepositorInsertDmMTOR (Mtor Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmMTOR_AA
Plasmid#148214PurposeInsect Expression of DmMTORDepositorInsertDmMTOR (Mtor Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5-LAP-MPS1-d200
Plasmid#114047PurposeExpression of exogenous LAP tagged MPS1 delta 200 (NTE and TPR are deleted)DepositorInsertMPS1-d200 (TTK Human)
UseTagsExpressionMammalianMutationdelta 200 (deletion of NTE and TPR domains)PromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBJG1 T7-8xHis-OGT 5N5A
Plasmid#154280PurposeBacterial expression of full length human OGT with 5N5A TPR mutations (inefficient substrate binding). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.DepositorInsertO-GlcNAc Transferase (OGT Human)
UseTags8x His, HRV3C protease site, and T7 leaderExpressionBacterialMutationN322A, N356A, N390A, N424A, N458A mutations; bact…PromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTU424
Plasmid#178983PurposepACYC containing Candidatus “Scalindua brodae” TPR-CHAT (codon-optimized)DepositorInsertTPR-CHAT
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTU425
Plasmid#178984PurposepACYC containing Candidatus “Scalindua brodae” TPR-CHAT H585A C627A (codon-optimized)DepositorInsertTPR-CHAT H585A C627A
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLacI-GFP-HEC1 WT
Plasmid#114049PurposeExpression of exogenous LacI-GFP-HEC1 WTDepositorInsertGFP-HEC1 (NDC80 Human)
UseTagsExpressionMammalianMutationWT HEC1 sequencePromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLacI-GFP-HEC1-d207
Plasmid#114050PurposeExpression of exogenous LacI-GFP-HEC1 delta 207DepositorInsertGFP-HEC1-d207 (NDC80 Human)
UseTagsExpressionMammalianMutationHEC1 delta 207PromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-hygro-eGFP-HEC1-d207
Plasmid#114051PurposeExpression of exogenous eGFP HEC1 delta 207DepositorInserteGFP-HEC1-d207 (NDC80 Human)
UseTagsExpressionMammalianMutationHEC1 delta 207PromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only