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pMOD_B-Pho2-1/2-2-tRNA

Plasmid

#161761

Purpose

MODULE B tRNA vector with 6x gRNAs targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pMOD_B2303 - #91068)

Depositor

Shaun Curtin

Article

Miller et al G3 (Bethesda). 2022 May 30;12(6). pii: 6

Insert

gRNAs

Use

Tags

Expression

Bacterial

Mutation

Promoter

CmYLCV Promoter

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pMOD_B-Pho2-1/2-2-Csy4

Plasmid

#161760

Purpose

MODULE B Csy4 vector with 6x gRNAs targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pMOD_B2103 - #91061)

Depositor

Shaun Curtin

Article

Miller et al G3 (Bethesda). 2022 May 30;12(6). pii: 6

Insert

gRNAs

Use

Tags

Expression

Bacterial

Mutation

Promoter

CmYLCV Promoter

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGE010-Target1 (Mpphot)

Plasmid

#186728

Purpose

Binary vector for CRISPR/Cas9 (target 1: Mpphot [positive control]) in plants (for Agrobacterium-mediated genetic transformation).

Depositor

Yutaka Kodama

Article

Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

Insert

Mphot

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGE010-Target2 (Mpphot)

Plasmid

#186729

Purpose

Binary vector for CRISPR/Cas9 (target 2: Mpphot [negative control]) in plants (for Agrobacterium-mediated genetic transformation).

Depositor

Yutaka Kodama

Article

Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

Insert

Mphot

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGE_En03-sgRNA_Target2 (Mpphot)

Plasmid

#186727

Purpose

Gateway entry vector for sgRNA (target 2: Mpphot [negative control]). Transient expression of sgRNA (target 2: Mpphot) in plant cells.

Depositor

Yutaka Kodama

Article

Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

Insert

sgRNA_Mphot

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGE_En03-sgRNA_Target1 (Mpphot)

Plasmid

#186726

Purpose

Gateway entry vector for sgRNA (target 1: Mpphot [positive control]). Transient expression of sgRNA (target 1: Mpphot) in plant cells.

Depositor

Yutaka Kodama

Article

Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

Insert

sgRNA_Mphot

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pHCKAn-phoBp-GRFT

Plasmid

#158746

Purpose

low phosphate phoBp induction of Griffithsin

Depositor

Michael Lynch

Article

Decker et al Front Bioeng Biotechnol. 2020 Aug 21;8:1

Insert

phoBp Griffithsin

Use

Tags

Expression

Bacterial

Mutation

Promoter

phoB

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_Sce-Acid Phosphatase SP

Plasmid

#153494

Purpose

S. cerevisiae Acid Phosphatase Signal Peptide Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

Acid Phosphatase SP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pHCKan-phoEp-GFPuv

Plasmid

#139074

Purpose

Low phosphate inducible promoter phoEp for GFP expression

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

phoEp-GFPuv

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-phoHp1-GFPuv

Plasmid

#139075

Purpose

Low phosphate inducible promoter phoHp1 for GFP expression

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

phoHp-GFPuv

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-phoUp-GFPuv

Plasmid

#139076

Purpose

Low phosphate inducible promoter phoUp for GFP expression

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

phoUp-GFPuv

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Synapsin-pHoenix-WPRE

Plasmid

#70111

Purpose

lentiviral expression of pHoenix (Synaptophysin-pHluorin-Arch3-mKate2) driven by human Synapsin I promoter for optogenetic acidification of synaptic vesicles

Depositor

Christian Rosenmund

Article

Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

Insert

pHoenix (Syp Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Rat)

Use

Lentiviral

Tags

Arch3, H+/K+ ATPase beta-subunit, mKate2, and pHl…

Expression

Mutation

Promoter

Synapsin

Available since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-NES-PhoCl2f-mCherry

Plasmid

#164048

Purpose

Optogenetic control of protein localization by PhoCl2f

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

NES-PhoCl2f-mCherry

Use

Tags

kozak sequence

Expression

Mammalian

Mutation

Promoter

CMV promotor

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-NES-PhoCl-mCherry

Plasmid

#87690

Purpose

Express NES-PhoCl-mCherry in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

NES-PhoCl-mCherry

Use

Tags

nuclear export signal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-PHOX2B-MUT

Plasmid

#194577

Purpose

Mammalian Expression of mEGFP-PHOX2B Mutant (S207Afs*102) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

PHOX2B (PHOX2B Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

S207Afs*102

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pfa6a PhoCl-TsCC(B)::KANMX

Plasmid

#185906

Purpose

Plasmid for cloning PhoCl fused to TsCC(B) into yeast.

Depositor

Matthew Good

Article

Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

Insert

PhoCl-TsCC(B)

Use

Tags

PhoCl fluorophore with C-terminal TsCC(B)

Expression

Yeast

Mutation

Promoter

SP6

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-NBid-PhoCl2c-CBid

Plasmid

#164051

Purpose

Optogenetic manipulation of cell apoptosis using PhoCl2c

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

NBid-PhoCl2c-CBid

Use

Tags

kozak sequence

Expression

Mammalian

Mutation

Promoter

CMV promotor

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcNDA-NES-PhoCl2c-mCherry

Plasmid

#164037

Purpose

Optogenetic control of protein localization by PhoCl2c

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

NES-PhoCl2c-mCherry

Use

Tags

kozak sequence

Expression

Mammalian

Mutation

Promoter

CMV promotor

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-NLS-PhoCl-mCherry

Plasmid

#87691

Purpose

Express NLS-PhoCl-mCherry in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

NLS-PhoCl-mCherry

Use

Tags

nuclear localization signal

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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delCMV-mCherry-3x-p67phox-GBD

Plasmid

#214141

Purpose

Rac activity sensor

Depositor

Leif Dehmelt

Article

Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

Insert

3xp67phox-GBD (NCF2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Only the GTPase binding domain (GBD) is included

Promoter

delCMV

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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