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pZR158_Lenti-U6-gRNA-10xCS1-PP7_hPGK-PCP-P65-HSF1-Puro

Plasmid

#180273

Purpose

Lentiviral expression vector for CRISPRa-SAM sgRNA with 10x capture sequence, and PCP-P65-HSF1 cassette

Depositor

Alexander Marson

Publication

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-FGFR1

Plasmid

#23922

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FGFR1 (FGFR1 Human)

Use

Gateway Cloning

Mutation

W211G and S351F compared to insert sequence provi…

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCRISPRa_all-in-one

Plasmid

#183695

Purpose

Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

Depositor

Edward Avezov

Publication

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Type

Empty backbone

Use

CRISPR

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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EPHA1 (3HIL)

Plasmid

#25322

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

epha1.911.974 (EPHA1 Human)

Tags

His

Expression

Bacterial

Available since

Aug. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pHAGE-FGFR2-N549K

Plasmid

#116380

Purpose

Lentiviral expression of FGFR2 N549K

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FGFR2 (FGFR2 Human)

Use

Lentiviral

Mutation

N549K

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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lenti-SAM-hygro

Plasmid

#138955

Purpose

Lentiviral vector for co-expressing spCas9, dRNA-MS2, p65 and HSF1 fused to MS2 binding protein, and hygromycin resistance gene

Depositor

Nadya Dimitrova

Publication

Olivero et al Mol Cell. 2020 Feb 20;77(4):761-774.e8.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

U6 AND EF1a

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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MAC_C_FGFR2

Plasmid

#187751

Purpose

MAC-tagged gene expression of human FGFR2

Depositor

Markku Varjosalo

Publication

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

FGFR2 (FGFR2 Human)

Expression

Mammalian

Available since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pBp-FGFR2b-K660E

Plasmid

#45705

Depositor

Matthew Meyerson

Publication

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIb (FGFR2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

K660E

Available since

June 24, 2013

Availability

Academic Institutions and Nonprofits only
pSAM2_mCherry_Baf60c

Plasmid

#72691

Purpose

Doxycyline regulated expression of Baf60c in mammalian cells

Depositor

Timothy Kamp

Publication

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Brg 1 (SMARCD3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MoFGFR2b-ires neo

Plasmid

#86491

Purpose

mamalian cell expression of FGFR2b

Depositor

David Ornitz

Publication

Ornitz et al J Biol Chem. 1996 Jun 21;271(25):15292-7

Insert

FgfrR2 IIIb 13A4 (FGFR2 Human)

Expression

Mammalian

Promoter

Moloney murine leukemia virus LTR

Available since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Mesp1

Plasmid

#72687

Purpose

Doxycyline regulated expression of Mesp1 in mammalian cells

Depositor

Timothy Kamp

Publication

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Mesp 1 (MESP1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBp-FGFR2b-P253R

Plasmid

#45716

Depositor

Matthew Meyerson

Publication

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIb (FGFR2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

P253R

Available since

June 27, 2013

Availability

Academic Institutions and Nonprofits only
pBp-FGFR2c-K660N

Plasmid

#45708

Depositor

Matthew Meyerson

Publication

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIc (FGFR2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

K660N

Available since

June 24, 2013

Availability

Academic Institutions and Nonprofits only
pBp-FGFR2b-K660N

Plasmid

#45707

Depositor

Matthew Meyerson

Publication

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIb (FGFR2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

K660N

Available since

June 24, 2013

Availability

Academic Institutions and Nonprofits only
pBp-FGFR2b-W290C

Plasmid

#45700

Depositor

Matthew Meyerson

Publication

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIb (FGFR2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

W290C

Available since

June 19, 2013

Availability

Academic Institutions and Nonprofits only
pBp-FGFR2c-W290C

Plasmid

#45701

Depositor

Matthew Meyerson

Publication

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIc (FGFR2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

W290C

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only
pAEM7

Plasmid

#136422

Purpose

salL gene from S. tropica CNB-440 ligated into pHIS8

Depositor

Bradley Moore

Publication

Eustaquio et al Nat Chem Biol. 2008 Jan;4(1):69-74. doi:

Insert

salL

Tags

His8 tag

Expression

Bacterial

Promoter

T7

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSAM2_mCherry_Isl1

Plasmid

#72696

Purpose

Doxycyline regulated expression of Isl1 in mammalian cells

Depositor

Timothy Kamp

Publication

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Islet1 (Isl1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRK5-HA-SAMTOR

Plasmid

#100513

Purpose

Expresses HA tagged SAMTOR in mammalian cells

Depositor

David Sabatini

Publication

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

SAMTOR (BMT2 Human)

Tags

HA

Expression

Mammalian

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-2T Sam68

Plasmid

#17687

Depositor

David Shalloway

Publication

Resnick et al Oncogene. 1997 Sep;15(11):1247-53.

Insert

Sam68 (Khdrbs1 Mouse)

Tags

GST

Expression

Bacterial

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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