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cACTG1

Plasmid

#236543

Purpose

For expression of ACTG1 in mammalian cells

Depositor

Sandra Citi

Article

Mauperin et al Nat Commun. 2025 Mar 13;16(1):2514. doi:

Insert

ACTG1

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

hpACTB

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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EcNusGKOW

Plasmid

#113124

Purpose

Expresses C-terminus of E. coli NusG

Depositor

James Berger

Article

Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

Insert

NusG (nusG Escherichia coli)

Use

Tags

His6-MBP-N10-TEV

Expression

Bacterial

Mutation

Deleted residues 1-124

Promoter

T7

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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EcRfaHKOW

Plasmid

#113125

Purpose

Expresses C-terminus of E. coli RfaH

Depositor

James Berger

Article

Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

Insert

RfaH (rfaH Escherichia coli)

Use

Tags

His6-MBP-N10-TEV

Expression

Bacterial

Mutation

Deleted residues 1-107

Promoter

T7

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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ARHGEF11(DHPH)-CRY2-mCherry

Plasmid

#89481

Purpose

Also known as optoGEF-RhoA, encodes for a fusion between CRY2(1-498)-mCherry and the DHPH domain of ARHGEF11

Depositor

Xavier Trepat Guixer

Article

Valon et al Nat Commun. 2017 Feb 10;8:14396. doi: 10

Insert

ARHGEF11(DHPH) - CRY2(1-498) - mCherry (CRY2, ARHGEF11 Human, Mustard Weed)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pTRE3G-FLAG-human cGAS N-T2A-deltaN-HA

Plasmid

#186886

Purpose

Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Human cGAS with T2A ribosome skipping sequences between N-terminus (1-159 a.a.) and C-terminus (160-522 a.a.) (Adamts1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mutation

Insertion of T2A ribosome skipping site

Promoter

TRE3G

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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xfz2N/pCS2+

Plasmid

#16329

Depositor

Peter Klein

Article

Deardorff et al Mech Dev. 1999 Sep . 87(1-2):229-33.

Insert

frizzled-2 (fzd2 Frog)

Use

Vertebrate expression

Tags

Expression

Mutation

Extracellular ligand binding domain of Xenopus fr…

Promoter

Available since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only
pGEX6P1-optimizedNedd4.2d

Plasmid

#165103

Purpose

bacterial expression of a GST fusion of Human Nedd4.2d

Depositor

Arthur Haas

Article

Todaro et al J Biol Chem. 2017 Nov 24;292(47):19521-1

Insert

Nedd4.2d (NEDD4L Human)

Use

Tags

GST from plasmid.

Expression

Bacterial

Mutation

codon optimized for E. coli expression

Promoter

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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F40 tension sensor

Plasmid

#101252

Purpose

Expresses the F40-based tension sensor module. The biosensor allows force measurements at 1-6 pN.

Depositor

Carsten Grashoff

Article

Austen et al Nat Cell Biol. 2015 Dec;17(12):1597-606.

Insert

YPet(short)-F40-mCherry

Use

Retroviral

Tags

His-tag and cysteine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 2, 2017

Availability

Academic Institutions and Nonprofits only

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myristoylated ct-GRK2

Plasmid

#178734

Purpose

N-myristoylation signaling peptide of Src and the C-terminal fragment of G-protein coupled receptor kinase 2 (ct-GRK2)

Depositor

Ege Kavalali

Article

Alten et al Cell Rep. 2022 Jan 18;38(3):110255. doi:

Insert

GRK2 (GRK2 Bovine)

Use

Lentiviral

Tags

Src 1-15

Expression

Mutation

only amino acids 548-671

Promoter

Ubiquitin

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Lyn11C3S-EGFP

Plasmid

#202747

Purpose

weakly plasma membrane targeted fluorescent protein

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

LYN (1-11) (C3S):EGFP (LYN Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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