We narrowed to 42,542 results for: cha;

1. pFA6a-kanMX6-PGAL1-GFP

   Plasmid

   #41614

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   GAL1 promoter (GAL1 Budding Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   Promoter

   Available since

   Dec. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. HM3C NaVAe1_p pET24b

   Plasmid

   #127854

   Purpose

   Bacterial expression vector for NaVAe1 bacterial sodium channel residues 143-288. It expresses HIStag-MBP-3C-channel protein.

   Depositor

   Dan Minor

   Article

   Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

   Insert

   NaVAe1p
   

   Use

   Tags

   3C Cleavage site, His, and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRPL28(K55M) SpHIS5 (pNTI666)

   Plasmid

   #115436

   Purpose

   Expresses K55M RPL28 from HIS3 yeast plasmid

   Depositor

   Nicholas Ingolia

   Article

   McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

   Inserts

   RPL28 (RPL28 Budding Yeast)
   HIS5
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   K55M; MfeI site in intron

   Promoter

   Ashbya gospii TEF1 and endogenous

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJT11

   Plasmid

   #98308

   Purpose

   Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Citrus limon limonene synthase LIS1 under the control of GAL1/GAL2 promoter

   Depositor

   Claudia Vickers

   Article

   Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

   Insert

   TRPL3ClLIS1>TRPL41B
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFA6a-His3MX6-PGAL1-3HA

   Plasmid

   #41610

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   GAL1 promoter (GAL1 Budding Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   3xHA, A. gossypii translation elongation factor 1…

   Expression

   Mutation

   Promoter

   Available since

   Dec. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pIRTetR-GAL80

   Plasmid

   #185853

   Purpose

   Plugging the mechanism of tetracycline-mediated repression of GAL promoters in Saccharomyces cerevisiae

   Depositor

   Claudia Vickers

   Article

   Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

   Insert

   gal80Arm1-PAgTEF1-KanMX4-TAgTEF1-PHAC1>TetR>TUP1>PABF1- PTEF1+[TetO]>GAL80Arm2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HpMscS

   Plasmid

   #78310

   Purpose

   H. pylori MscS with N-Term 6x Hisitidine Tag and C-Term FLAG Tag

   Depositor

   Douglas Rees

   Article

   Lai et al Protein Sci. 2013 Apr;22(4):502-9. doi:

   Insert

   Mechanosensitive channel of small conductance
   

   Use

   Tags

   6x His Tag and FLAG Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJT10R

   Plasmid

   #98307

   Purpose

   Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of GAL1/GAL2 promoter

   Depositor

   Claudia Vickers

   Article

   Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

   Insert

   TRPL3AcNES1>TRPL41B
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   ERG20 N127W

   Promoter

   Available since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJT2

   Plasmid

   #98306

   Purpose

   Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoter

   Depositor

   Claudia Vickers

   Article

   Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

   Insert

   PTEF2>ScERG20N127W>TRPL3>PTEF1>AcNES1>TRPL41B
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   ERG20 N127W

   Promoter

   Available since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HM3C NaVSp1_p pET24b

    Plasmid

    #127852

    Purpose

    Bacterial expression vector for NaVSp1 bacterial sodium channel residues 122-258. It expresses HIStag-MBP-3C-channel protein.

    Depositor

    Dan Minor

    Article

    Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

    Insert

    NaVSp1
    

    Use

    Tags

    3C Cleavage site, His Tag, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV CaMKIIa ChR2 E90R D156N T159C 2A tDimer

    Plasmid

    #52878

    Purpose

    Chloride-conducting Channelrhodopsin with slow kinetics, neuron-specific promoter, plus red fluorescent protein, excitatory neurons

    Depositor

    Thomas Oertner

    Article

    Wietek et al Science. 2014 Mar 27.

    Inserts

    Channelrhodopsin-2
    red fluorescent protein
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    E90R, D156N, T159C

    Promoter

    CaMKIIa

    Available since

    July 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. myc-SpELL pBacPAK8

    Plasmid

    #15318

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

    Insert

    eleven nineteen lysine rich in leukemia (ell1 Fission Yeast)
    

    Use

    Baculovirus construction

    Tags

    Myc

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

13. FLAG-SpEAF 60-251 pBacPAK8

    Plasmid

    #15321

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

    Insert

    EAF1 (eaf1 Fission Yeast)
    

    Use

    Baculovirus construction

    Tags

    FLAG

    Expression

    Mutation

    N terminal deletion lacking first 59 amino acids …

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

14. MCUb-mVenus-pLJM

    Plasmid

    #236789

    Purpose

    MCUB tagged with mVenus for FRET / MW change for stoichiometry

    Depositor

    Dipayan Chaudhuri

    Article

    Rai et al J Cell Physiol. 2025 Apr;240(4):e70033.

    Insert

    MCUB (MCUB Human)
    

    Use

    Lentiviral

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJET-LB-geneticin

    Plasmid

    #213468

    Purpose

    Vector to amplify the LB-geneticin fragment for transformation into TSI locus 1

    Depositor

    Kim Hammond-Kosack

    Article

    Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

    Insert

    Left border of the TSI locus 1-split fragment of geneticin cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPG-GPD-TRPC

    Plasmid

    #190113

    Purpose

    Expression vector for fungal (Aspergillus flavus) genes based on Aspergillus parasiticus pyrG gene selection

    Depositor

    Perng-Kuang Chang

    Article

    Chang et al Fungal Genet Biol. 2017 Jul;104:29-37. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-ybbr-XMod-Doc (WT, S199AzF)-HIS

    Plasmid

    #153443

    Purpose

    E. coli expression (amber suppression) of Rc. XDocB with serine at position 199 replaced with amber codon.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.XDocB
    

    Use

    Tags

    6xHis and ybbr tag

    Expression

    Bacterial

    Mutation

    Serine 199 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMH-HT_ygiF-TTM

    Plasmid

    #127794

    Purpose

    Expresses the TTM/CYTH domain of ygiF in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    ygiF (ygiF Escherichia coli)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    N-terminal TTM domain of ygiF (residues 1-200)

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRPL28 SpHIS5 (pNTI663)

    Plasmid

    #115433

    Purpose

    Expresses wild-type RPL28 from HIS3 yeast plasmid

    Depositor

    Nicholas Ingolia

    Article

    McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

    Inserts

    RPL28 (RPL28 Budding Yeast)
    HIS5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    MfeI site in intron

    Promoter

    Ashbya gospii TEF1 and endogenous

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRPL28(Q38P) SpHIS5 (pNTI664)

    Plasmid

    #115434

    Purpose

    Expresses Q38P RPL28 from HIS3 yeast plasmid

    Depositor

    Nicholas Ingolia

    Article

    McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

    Inserts

    RPL28 (RPL28 Budding Yeast)
    HIS5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Q38P; MfeI site in intron

    Promoter

    Ashbya gospii TEF1 and endogenous

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRPL28(H39L) SpHIS5 (pNTI665)

    Plasmid

    #115435

    Purpose

    Expresses H39L RPL28 from HIS3 yeast plasmid

    Depositor

    Nicholas Ingolia

    Article

    McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

    Inserts

    RPL28 (RPL28 Budding Yeast)
    HIS5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H39L; MfeI site in intron

    Promoter

    ashbya gospii TEF1 and endogenous

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFA6a-His3MX6-PGAL1-GST

    Plasmid

    #41613

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJC25R1+4stop

    Plasmid

    #17723

    Depositor

    Susan Lindquist

    Article

    Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

    Insert

    Sup35R1+4 (SUP35 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E.coli expression plasmid for NM-region of Sup35 …

    Promoter

    Available since

    April 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. FLAG-SpELL pNMT-TOPO

    Plasmid

    #15315

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

    Insert

    eleven nineteen lysine rich in leukemia (ell1 Fission Yeast)
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

25. FLAG-SpEAF pNMT-TOPO

    Plasmid

    #15316

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

    Insert

    ELL associated factor (eaf1 Fission Yeast)
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

26. FLAG-SpELL pBacPAK8

    Plasmid

    #15317

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

    Insert

    eleven nineteen lysine rich in leukemia (ell1 Fission Yeast)
    

    Use

    Baculovirus construction

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

27. KA1042_pPB-hCMV*1-Esrrb-IV

    Plasmid

    #124177

    Purpose

    piggyBac-based Dox-inducible expression of Esrrb

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Esrrb-IRES-Venus (Esrrb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO-CMVmin

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. KA2880_pPB-TREtight-FLAG-Esrrb-InO

    Plasmid

    #124178

    Purpose

    piggyBac-based Dox-inducible expression of FLAG-Esrrb

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    FLAG-Esrrb-IRES-H2B-mKO2 (Esrrb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO-CMVmin

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. KA2944_pPB-TREtight-Esrrb-FLAG-InO

    Plasmid

    #124179

    Purpose

    piggyBac-based Dox-inducible expression of Esrrb-FLAG

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Esrrb-FLAG-IRES-H2B-mKO2 (Esrrb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO-CMVmin

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PLJR962

    Plasmid

    #115162

    Purpose

    Sth1 dCas9 TetR and KanR L5 Int attP for M. smegmatis

    Depositor

    Sarah Fortune

    Article

    Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

    Inserts

    Sth1 sgRNA scaffold
    Sth1 dCas9
    TetR
    L5 attP
    L5 Int
    KanR
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. PLJR965

    Plasmid

    #115163

    Purpose

    Sth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosis

    Depositor

    Sarah Fortune

    Article

    Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

    Inserts

    Sth1 sgRNA scaffold
    Sth1 dCas9
    TetR (deleted TetON) MtbCO
    L5 attP
    L5 Int
    KanR
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. EGFP-CLC

    Plasmid

    #193559

    Purpose

    Expresses human Clathrin Light Chain B tagged with mEGFP in mammalian cells.

    Depositor

    Justin Taraska

    Article

    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

    Insert

    Clathrin light chain B (CLTB Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. HMBP-3C-STAR.66-285

    Plasmid

    #100094

    Purpose

    expresses MBP-tagged STARD1 protein domain (residues 66-285)

    Depositor

    Nikolai Sluchanko

    Article

    Sluchanko et al Protein Expr Purif. 2016 Mar;119:27-35.

    Insert

    STAR protein (STAR Human)
    

    Use

    Tags

    His-tagged MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1(+)_Pmel-1_TCRB

    Plasmid

    #175288

    Purpose

    Expresses Pmel-1 TCR beta chain

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    T cell receptor beta chain (Tcrb Mouse)
    

    Use

    Suitable for ivt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3.1(+)_Pmel-1_TCRA

    Plasmid

    #175287

    Purpose

    Expresses Pmel-1 TCRA alpha chain

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    T cell receptor alpha chain (Tcra Mouse)
    

    Use

    Suitable for ivt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. cacnb2a (rat) in pMT2 vector

    Plasmid

    #107424

    Purpose

    Expresses Calcium channel beta2a auxillary subunit in mammalian cells

    Depositor

    Annette Dolphin

    Article

    Wyatt et al J Physiol. 1998 Jul 15;510 ( Pt 2):347-6

    Insert

    calcium channel beta2a auxillary subunit (Cacnb2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ad MLP

    Available since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA SF-HERC2 F2

    Plasmid

    #55785

    Purpose

    Expresses N-terminal 3xFLAG/STREP tagged HERC2 (950-1750aa)

    Depositor

    David Chan

    Article

    Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

    Insert

    3xFLAG tagged HERC2 (950aa-1750aa) (HERC2 Human)
    

    Use

    Tags

    3xFLAG and STREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 23, 2014

    Availability

    Academic Institutions and Nonprofits only

38. pAAV-EF1a-DIO-H2B.APEX-P2A-EGFP

    Plasmid

    #182825

    Purpose

    Cre-dependent expression of H2B-APEX fusion

    Depositor

    Yevgenia Kozorovitskiy

    Article

    Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

    Insert

    H2B.APEX-P2A-EGFP
    

    Use

    AAV

    Tags

    EGFP reporter (P2A)

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pFA6a-His3MX6-PGAL1-GFP

    Plasmid

    #41616

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTracer_Cr-TRP1

    Plasmid

    #64881

    Purpose

    Expresses CrTRP1 in mammalian cells

    Depositor

    Sebastián Brauchi

    Article

    Arias-Darraz et al Plant Cell. 2015 Jan;27(1):177-88. doi:

    Insert

    Transient receptor potential 1
    

    Use

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only