We narrowed to 10,722 results for: Kars

1. A365Y-GFP Donor for iWTr EED

   Plasmid

   #131324

   Purpose

   Donor to generate i-WT-r system

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   i-WT-r.Donor
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. EED-KO-gRNA-2

   Plasmid

   #131322

   Purpose

   gRNA to knockout EED. Use with EED-KO-gRNA-1

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   EED-KO-gRNA-2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

3. pLY133

   Plasmid

   #130961

   Purpose

   Engineered sgRNA-LEB4 generator including Anderson promoter J23100 for golden gate assembly

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Insert

   sgRNA-LEB4
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Anderson promoter J23100

   Available since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFA-dabAB2

   Plasmid

   #133012

   Purpose

   pFA carrying the badabA2 (locus_tag: BAS2958) and badabB2 genes (locus_tag: BAS2959)

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Inserts

   dabB2
   dabA2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tetR

   Available since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFE-dabAB2 C351A

   Plasmid

   #133005

   Purpose

   pFE carrying a dabA2 gene with a C351A mutation and a dabB2 gene (Uniprot: D0KWS8)

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Inserts

   dabB2
   dabA2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C351A

   Promoter

   tetR

   Available since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFE-baDAB

   Plasmid

   #133003

   Purpose

   pFE carrying the badabA and badabB genes

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Inserts

   badabB
   vcdabA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tetR

   Available since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLY86

   Plasmid

   #130949

   Purpose

   Engineered sgRNA-LEA2-WTmis2 generator circuit including promoter Plux2, 2 BoxB aptamers and 2 mis-matches in sgRNA scaffold

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Insert

   sgRNA-LEA2-WTmis2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plux2

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLY132

   Plasmid

   #130960

   Purpose

   Engineered sgRNA-LEB3 generator including Anderson promoter J23100 for golden gate assembly

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Insert

   sgRNA-LEB3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Anderson promoter J23100

   Available since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLY130

   Plasmid

   #130952

   Purpose

   Engineered sgRNA-LEB1 generator including Anderson promoter J23100 for golden gate assembly

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Insert

   sgRNA-LEB1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Anderson promoter J23100

   Available since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMV306-ribo-Sec-APEX2

    Plasmid

    #111701

    Purpose

    Theophylline-inducible expression of periplasmic APEX2 from a mycobacterial shuttle plasmid (integrating)

    Depositor

    Jessica Seeliger

    Article

    Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

    Insert

    APEX2
    

    Use

    Tags

    V5 and mpt63 signal sequence

    Expression

    Bacterial

    Mutation

    Promoter

    hsp60-ribo

    Available since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a-YJR129c

    Plasmid

    #85118

    Purpose

    bacterial expression of YJR129c

    Depositor

    Pal Falnes

    Article

    Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

    Insert

    EFM3 (EFM3 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTriEX Tom20 mVenus BoPD

    Plasmid

    #114466

    Purpose

    Mammalian expression of mitochondrially targeted, mVenus labelled BoPD

    Depositor

    Andrew Woolley

    Article

    Reis et al ACS Synth Biol. 2018 Oct 19;7(10):2355-2

    Insert

    BoPD
    

    Use

    Tags

    HIS, TEV and FLAG and TOM20 and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLPC-Flag-TZAPZNF1-8

    Plasmid

    #87181

    Purpose

    retroviral stable expression of truncation protein Flag-TZAPZNF1-8

    Depositor

    Eros Lazzerini Denchi

    Article

    Li et al Science. 2017 Jan 12. pii: aah6752. doi:

    Insert

    TZAP (ZBTB48 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLPC-Flag-TZAPZNF7-9

    Plasmid

    #87183

    Purpose

    retroviral stable expression of truncation protein Flag-TZAPZNF7-9

    Depositor

    Eros Lazzerini Denchi

    Article

    Li et al Science. 2017 Jan 12. pii: aah6752. doi:

    Insert

    TZAP (ZBTB48 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLPC-Flag-TZAPZNF7-11

    Plasmid

    #87182

    Purpose

    retroviral stable expression of truncation protein Flag-TZAPZNF7-11

    Depositor

    Eros Lazzerini Denchi

    Article

    Li et al Science. 2017 Jan 12. pii: aah6752. doi:

    Insert

    TZAP (ZBTB48 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDNA-H1-sgRNA-mTZAP

    Plasmid

    #87187

    Purpose

    guideRNA targeting exon2 of mouse TZAP

    Depositor

    Eros Lazzerini Denchi

    Article

    Li et al Science. 2017 Jan 12. pii: aah6752. doi:

    Insert

    TZAP (Zbtb48 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    March 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR221-OSF-TRIM5-21R(1-300)

    Plasmid

    #79029

    Purpose

    Gateway entry vector for cloning and propagation in DH5alpha

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    TRIM5-21R (1-300)
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    Promoter

    T7

    Available since

    March 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. BC-A1-016

    Plasmid

    #78704

    Purpose

    A weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a weak RBS.

    Depositor

    Brian Chow

    Article

    Magaraci et al ACS Synth Biol. 2016 Apr 25.

    Insert

    BBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0033-GFP-Terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. BC-A1-015

    Plasmid

    #78703

    Purpose

    A weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a weak RBS.

    Depositor

    Brian Chow

    Article

    Magaraci et al ACS Synth Biol. 2016 Apr 25.

    Insert

    BBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. BC-A1-010

    Plasmid

    #78697

    Purpose

    A strong promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a strong RBS.

    Depositor

    Brian Chow

    Article

    Magaraci et al ACS Synth Biol. 2016 Apr 25.

    Insert

    BBa_J23100-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP-Terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. BC-A1-008

    Plasmid

    #78695

    Purpose

    A weak promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a weak RBS.

    Depositor

    Brian Chow

    Article

    Magaraci et al ACS Synth Biol. 2016 Apr 25.

    Insert

    BBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP-Terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. BC-A1-007

    Plasmid

    #78694

    Purpose

    A weak promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a weak RBS.

    Depositor

    Brian Chow

    Article

    Magaraci et al ACS Synth Biol. 2016 Apr 25.

    Insert

    BBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJK278

    Plasmid

    #71134

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H89N mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-89 to asparagine; additional N-…

    Promoter

    T7

    Available since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJK130

    Plasmid

    #71129

    Purpose

    Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide synthetase (PurK) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changed valine-319 to phenylalanine

    Promoter

    T7

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJK173

    Plasmid

    #71831

    Purpose

    Cloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE, and purK genes

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Inserts

    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJK320

    Plasmid

    #71144

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H59S mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-59 to serine; additional N-term…

    Promoter

    T7

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJK319

    Plasmid

    #71143

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H59A mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-59 to alanine; additional N-ter…

    Promoter

    T7

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJK318

    Plasmid

    #71142

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H59Q mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-59 to glutamine; additional N-t…

    Promoter

    T7

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJK315

    Plasmid

    #71141

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H89G mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-89 to glycine; additional N-ter…

    Promoter

    T7

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJK314

    Plasmid

    #71140

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H89V mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-89 to valine; additional N-term…

    Promoter

    T7

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pJK174

    Plasmid

    #71835

    Purpose

    Expression of Acetobacter aceti 1023 PurE and PurK in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Inserts

    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    additional N-terminal methionine encoded by this …

    Promoter

    T7 and endogenous

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pJK279

    Plasmid

    #71135

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H89D mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-89 to aspartate; additional N-t…

    Promoter

    T7

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pJK283

    Plasmid

    #71139

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H59N mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-59 to asparagine; additional N-…

    Promoter

    T7

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJK282

    Plasmid

    #71138

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H59D mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-59 to aspartate; additional N-t…

    Promoter

    T7

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pJK281

    Plasmid

    #71137

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H59F mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-59 to phenylalanine; additional…

    Promoter

    T7

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJK280

    Plasmid

    #71136

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H89F mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-89 to phenylalanine; additional…

    Promoter

    T7

    Available since

    Jan. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pJK175

    Plasmid

    #71132

    Purpose

    Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase (class I PurE) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    additional N-terminal methionine encoded by this …

    Promoter

    T7

    Available since

    Jan. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTH729-minRLuc

    Plasmid

    #38225

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Insert

    Renilla Luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Every codon of the Renilla coding sequence was ex…

    Promoter

    TDH3 (=GPD)

    Available since

    Dec. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTH738-CENBEVYslow

    Plasmid

    #38220

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Insert

    GCN4-based single uORF-containing 5'-UTR
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    The start codons of uORFs2, 3 and4 of the yeast G…

    Promoter

    TDH3 (=GPD)

    Available since

    Dec. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. human pirh2-(138-261)

    Plasmid

    #24882

    Depositor

    Cheryl Arrowsmith

    Article

    Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

    Insert

    Pirh2 (RCHY1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains aa 138-261.

    Promoter

    Available since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

41. human pirh2-(187-261)

    Plasmid

    #24883

    Depositor

    Cheryl Arrowsmith

    Article

    Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

    Insert

    Pirh2 (RCHY1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains aa 187-261.

    Promoter

    Available since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

42. AIRAP 5: AIRAP(1-50).GST,V1.pcDNA3

    Plasmid

    #21795

    Depositor

    David Ron

    Article

    Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

    Insert

    AIRAP (Zfand2a Mouse)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    amino acids 1-50

    Promoter

    Available since

    Sept. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pLenti-UMI

    Plasmid

    #222694

    Purpose

    CRISPR-screening

    Depositor

    Ulrich Elling

    Article

    Michlits et al Nat Methods. 2017 Dec;14(12):1191-1197.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PGK without BsmBI cutsite

    Promoter

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. BPK1520

    Plasmid

    #65777

    Purpose

    Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    SpCas9 gRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. SIN40C.SFFV.Luciferase.IRES.GFP

    Plasmid

    #169308

    Purpose

    Constitutive overexpression of Luciferase cDNA with GFP as a reporter fluorescent protein

    Depositor

    Jan-Henning Klusmann

    Article

    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

    Insert

    Luciferase
    

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. 014LP-pAAV-ANF-657- Cre-pA

    Plasmid

    #120279

    Purpose

    AAV vector for Atrial specific Cre recombinase expression

    Depositor

    Xander Wehrens

    Article

    Ni et al Circ Res. 2019 Jan 18;124(2):256-262. do

    Insert

    Cre
    

    Use

    AAV

    Tags

    CRE

    Expression

    Mutation

    Promoter

    ANF promoter

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. PB_iMYOD1_P2A_GFP_Puro

    Plasmid

    #168804

    Purpose

    PiggyBac transposon construct with doxycycline inducible MYOD1 + GFP expression

    Depositor

    Prashant Mali

    Article

    Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

    Insert

    MYOD1 (MYOD1 Human, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pDF0159 pCMV - huDisCas7-11 mammalian expression

    Plasmid

    #172507

    Purpose

    Encodes human codon optimized DisCas7-11 protein alone for expression in mammalian cells

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    pCMV - huDisCas7-11 mammalian expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. lenti-NLS-miRFP670nano-miRFP670

    Plasmid

    #216131

    Purpose

    Lentiviral vector for expressing a fusion of miRFP670nano and miRFP670 localizing to the nucleus.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

    Insert

    EF1a-NuclearLocalizationSignal-miRFP670nano-miRFP670-WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. rs214-minor

    Plasmid

    #113380

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs2149943 minor allele (T)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs2149943 minor allele
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge