We narrowed to 15,723 results for: LEA

1. pOCC70

   Plasmid

   #118878

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3C

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-TEV-mGFP-3C-HIS6-stop-HindIII cassette
   

   Tags

   mGFP, cleavable with TEV protease; HIS6, cleavabl…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOCC204

   Plasmid

   #118894

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C and C-terminal mKate2, and HIS6, cleavable with 3C

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-MBP-3C-NotI-ccdB-AscI-mKate2-3C-HIS6-stop-HindIII cassette
   

   Tags

   MBP, cleavable with 3C protease and mKate2, HIS6,…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJOE6089.4

   Plasmid

   #135081

   Purpose

   pBR322-based L-rhamnose-inducible vector.

   Depositor

   Josef Altenbuchner

   Article

   Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

   Insert

   P-rhaP-BAD-eGFP-Strep-tag II
   

   Expression

   Bacterial

   Available Since

   Feb. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. TALEN-CCR5L18-CtermCan

   Plasmid

   #51438

   Purpose

   Expresses TALEN targeting left CCR5A site (L18) with canonical C-terminal domain

   Depositor

   David Liu

   Article

   Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

   Insert

   TALEN CCR5 Left (L18) Can C-term
   

   Use

   TALEN

   Tags

   3xFLAG and Fok1 EL variant

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. TALEN-CCR5R18-CtermCan

   Plasmid

   #51439

   Purpose

   Expresses TALEN targeting right CCR5A site (R18) with canonical C-terminal domain

   Depositor

   David Liu

   Article

   Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

   Insert

   TALEN CCR5 Right (R18) Can C-term
   

   Use

   TALEN

   Tags

   3xFLAG and Fok1 KK variant

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOCC230

   Plasmid

   #118871

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEV

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
   

   Tags

   HIS6, cleavable with 3C protease and mCitrine, cl…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOCC69

   Plasmid

   #118874

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal monomeric GFP, cleavable with TEV

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mGFP-stop-HindIII cassette
   

   Tags

   HIS6, cleavable with 3C protease and mGFP, cleava…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX4T-1_OCT4_S235A

   Plasmid

   #40639

   Depositor

   James Thomson

   Article

   Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

   Insert

   POU5F1 (POU5F1 Human)
   

   Tags

   GST, TEV Cleavage, TEV cleavage, Thrombin Cleavag…

   Expression

   Bacterial

   Mutation

   S235A

   Promoter

   tac

   Available Since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

9. TALEN-CCR5L18-NtermN2

   Plasmid

   #51446

   Purpose

   Expresses TALEN targeting left CCR5A site (L18) with N2 N-terminal domain

   Depositor

   David Liu

   Article

   Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

   Insert

   TALEN CCR5 Left (L18) N1 N-term
   

   Use

   TALEN

   Tags

   3xFLAG and Fok1 EL variant

   Expression

   Mammalian

   Mutation

   K150Q and K153Q

   Promoter

   CMV

   Available Since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. TALEN-CCR5R18-NtermN2

    Plasmid

    #51447

    Purpose

    Expresses TALEN targeting right CCR5A site (R18) with N2 N-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Right (R18) N2 N-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 KK variant

    Expression

    Mammalian

    Mutation

    K150Q and K153Q

    Promoter

    CMV

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2+-Zeo-miR-430-G-G mm

    Plasmid

    #221728

    Purpose

    Expression of Zeocin resistance marker followed by miR-430 target site insertion with G-G mismatch at position 6 in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    BleoR-miR-430-G-G mm
    

    Use

    Fish expression

    Mutation

    G-G mismatch of miR-430 site

    Promoter

    simian CMV IE94

    Available Since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2+-Zeo-miR-430-10-11 mm

    Plasmid

    #221727

    Purpose

    Expression of Zeocin resistance marker followed by miR-430 target site insertion with mismatches at position 10 and 11 in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    BleoR-miR-430-10-11 mm
    

    Use

    Fish expression

    Mutation

    Mismatch at positions 10 and 11 of miR-430 site

    Promoter

    simian CMV IE94

    Available Since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2+-drAgo2

    Plasmid

    #221711

    Purpose

    Expression of zebrafish Ago2 in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    drAgo2 (ago2 Zebrafish)
    

    Use

    Fish expression

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMCSG7-10xHis-ecRNHI*

    Plasmid

    #219523

    Purpose

    bacterial expression of cysteine-free variant of the E. coli ribonuclease HI gene

    Depositor

    Arthur Palmer

    Article

    Bhattacharya et al J Biomol NMR. 2024 Jun 10. doi: 10.1007/

    Insert

    cysteine-free variant of the E. coli ribonuclease HI gene
    

    Tags

    N terminal 10xHis Tag

    Expression

    Bacterial

    Mutation

    Cysteine free variant

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. KBB430

    Plasmid

    #185091

    Purpose

    NMA111-GFP with first nuclear localization signal mutated (amino acids 28 - 30, KRK -> AAA)

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    First nuclear localization signal mutated (amino …

    Available Since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. KBB428

    Plasmid

    #185090

    Purpose

    NMA111-GFP with first nuclear localization signal mutated (amino acids 9 - 11, KKR -> AAA)

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    First nuclear localization signal mutated (amino …

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-CMV-SMCHD1-Exon1-11E.12D-14.47M-48-3xFLAG

    Plasmid

    #130684

    Purpose

    Expresses human SMCHD1 Exon1-11E.12D-14.47M-48 with a C-terminal 3xFLAG tag

    Depositor

    Stephen Tapscott

    Article

    Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

    Insert

    SMCHD1 Exon1-11E.12D-14.47M-48 (SMCHD1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D453_F489del, E653_G1960del

    Promoter

    CMV

    Available Since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-CMV-SMCHD1-Exon1-10A.11D-14.47M-48-3xFLAG

    Plasmid

    #130683

    Purpose

    Expresses human SMCHD1 Exon1-10A.11D-14.47M-48 with a C-terminal 3xFLAG tag

    Depositor

    Stephen Tapscott

    Article

    Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

    Insert

    SMCHD1 Exon1-10A.11D-14.47M-48 (SMCHD1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D409_E452del, E653_G1960del

    Promoter

    CMV

    Available Since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCR8_TaNAM-A1

    Plasmid

    #124574

    Purpose

    TaNAM-A1 CDS in the Gateway-compatible pCR8 vector.

    Depositor

    Cristobal Uauy

    Article

    Harrington et al BMC Plant Biol. 2019 Sep 18;19(1):407. d

    Insert

    TaNAM-A1
    

    Use

    Topo gateway cloning vector

    Promoter

    None

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. Banshee-ShCit-A

    Plasmid

    #85216

    Purpose

    Short hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.

    Depositor

    Alex Minella

    Article

    Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002

    Insert

    shCit-A
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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