We narrowed to 15,548 results for: LEA

1. TALEN-CCR5R18-NtermN2

   Plasmid

   #51447

   Purpose

   Expresses TALEN targeting right CCR5A site (R18) with N2 N-terminal domain

   Depositor

   David Liu

   Article

   Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

   Insert

   TALEN CCR5 Right (R18) N2 N-term
   

   Use

   TALEN

   Tags

   3xFLAG and Fok1 KK variant

   Expression

   Mammalian

   Mutation

   K150Q and K153Q

   Promoter

   CMV

   Available since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2+-Zeo-miR-430-G-G mm

   Plasmid

   #221728

   Purpose

   Expression of Zeocin resistance marker followed by miR-430 target site insertion with G-G mismatch at position 6 in zebrafish embryos.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   BleoR-miR-430-G-G mm
   

   Use

   Fish expression

   Tags

   Expression

   Mutation

   G-G mismatch of miR-430 site

   Promoter

   simian CMV IE94

   Available since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2+-Zeo-miR-430-10-11 mm

   Plasmid

   #221727

   Purpose

   Expression of Zeocin resistance marker followed by miR-430 target site insertion with mismatches at position 10 and 11 in zebrafish embryos.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   BleoR-miR-430-10-11 mm
   

   Use

   Fish expression

   Tags

   Expression

   Mutation

   Mismatch at positions 10 and 11 of miR-430 site

   Promoter

   simian CMV IE94

   Available since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2+-drAgo2

   Plasmid

   #221711

   Purpose

   Expression of zebrafish Ago2 in zebrafish embryos.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   drAgo2 (ago2 Zebrafish)
   

   Use

   Fish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMCSG7-10xHis-ecRNHI*

   Plasmid

   #219523

   Purpose

   bacterial expression of cysteine-free variant of the E. coli ribonuclease HI gene

   Depositor

   Arthur Palmer

   Article

   Bhattacharya et al J Biomol NMR. 2024 Jun 10. doi: 10.1007/

   Insert

   cysteine-free variant of the E. coli ribonuclease HI gene
   

   Use

   Tags

   N terminal 10xHis Tag

   Expression

   Bacterial

   Mutation

   Cysteine free variant

   Promoter

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. KBB430

   Plasmid

   #185091

   Purpose

   NMA111-GFP with first nuclear localization signal mutated (amino acids 28 - 30, KRK -> AAA)

   Depositor

   Ken Belanger

   Article

   Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

   Insert

   NMA111
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   First nuclear localization signal mutated (amino …

   Promoter

   Available since

   July 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. KBB428

   Plasmid

   #185090

   Purpose

   NMA111-GFP with first nuclear localization signal mutated (amino acids 9 - 11, KKR -> AAA)

   Depositor

   Ken Belanger

   Article

   Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

   Insert

   NMA111
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   First nuclear localization signal mutated (amino …

   Promoter

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-CMV-SMCHD1-Exon1-11E.12D-14.47M-48-3xFLAG

   Plasmid

   #130684

   Purpose

   Expresses human SMCHD1 Exon1-11E.12D-14.47M-48 with a C-terminal 3xFLAG tag

   Depositor

   Stephen Tapscott

   Article

   Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

   Insert

   SMCHD1 Exon1-11E.12D-14.47M-48 (SMCHD1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D453_F489del, E653_G1960del

   Promoter

   CMV

   Available since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-CMV-SMCHD1-Exon1-10A.11D-14.47M-48-3xFLAG

   Plasmid

   #130683

   Purpose

   Expresses human SMCHD1 Exon1-10A.11D-14.47M-48 with a C-terminal 3xFLAG tag

   Depositor

   Stephen Tapscott

   Article

   Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

   Insert

   SMCHD1 Exon1-10A.11D-14.47M-48 (SMCHD1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D409_E452del, E653_G1960del

   Promoter

   CMV

   Available since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCR8_TaNAM-A1

    Plasmid

    #124574

    Purpose

    TaNAM-A1 CDS in the Gateway-compatible pCR8 vector.

    Depositor

    Cristobal Uauy

    Article

    Harrington et al BMC Plant Biol. 2019 Sep 18;19(1):407. d

    Insert

    TaNAM-A1
    

    Use

    Topo gateway cloning vector

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Banshee-ShCit-A

    Plasmid

    #85216

    Purpose

    Short hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.

    Depositor

    Alex Minella

    Article

    Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002

    Insert

    shCit-A
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Banshee-ShCit-B

    Plasmid

    #85217

    Purpose

    Short hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.

    Depositor

    Alex Minella

    Article

    Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002

    Insert

    shCit-B
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TALEN-CCR5L18-NtermN3

    Plasmid

    #51448

    Purpose

    Expresses TALEN targeting left CCR5A site (L18) with N3 N-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Left (L18) N3 N-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 EL variant

    Expression

    Mammalian

    Mutation

    K150Q, K153Q, and R154Q

    Promoter

    CMV

    Available since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TALEN-CCR5R18-NtermN3

    Plasmid

    #51449

    Purpose

    Expresses TALEN targeting right CCR5A site (R18) with N3 N-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Right (R18) N3 N-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 KK variant

    Expression

    Mammalian

    Mutation

    K150Q, K153Q, and R154Q

    Promoter

    CMV

    Available since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LBR EGFP

    Plasmid

    #128150

    Purpose

    Expression of lamin B receptor

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Ellenberg et al J Cell Biol. 1997 Sep 22. 138(6):1193-20

    Insert

    lamin B receptor (LBR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3 V5 mDispatched WT 2x HA

    Plasmid

    #126410

    Purpose

    Expression of V5 Dispatched WT HA in mammalian cell lines.

    Depositor

    Stacey Ogden

    Article

    Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

    Insert

    m-Dispatched1 (Disp1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    V5 iserted between A106 and A107

    Promoter

    Available since

    June 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lenti-eCas9

    Plasmid

    #140237

    Purpose

    Lentiviral vector for expression of high-specificity eSpCas9(1.1) and sgRNA scaffold

    Depositor

    Urs Greber

    Article

    Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a core

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS2+-FLAG3-drAgo2

    Plasmid

    #221712

    Purpose

    Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    drAgo2 (ago2 Zebrafish)
    

    Use

    Fish expression

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1 HisC EXO1b

    Plasmid

    #68268

    Purpose

    Mammalian expression of human EXO1

    Depositor

    Stefano Ferrari

    Article

    El-Shemerly et al Cancer Res. 2005 May 1;65(9):3604-9.

    Insert

    EXO1 (EXO1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    b form, codons 1-846. Please see depositor commen…

    Promoter

    CMV

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pM9-BetaGal-8C

    Plasmid

    #172489

    Purpose

    Plasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequence

    Depositor

    Siegfried Musser

    Article

    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

    Insert

    M9-BetaGal-8C
    

    Use

    Tags

    6xHis and M9 nuclear localization sequence (from …

    Expression

    Bacterial

    Mutation

    BetaGal has two cysteine mutations (C77A, C1022S)…

    Promoter

    T7

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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