We narrowed to 5,067 results for: codon optimized

1. pCMV-T7-SpRY-P2A-EGFP (RTW4830)

   Plasmid

   #139989

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

   Promoter

   CMV and T7

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)

   Plasmid

   #140003

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

   Promoter

   CMV and T7

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)

   Plasmid

   #139997

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

   Promoter

   CMV and T7

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE2-EF1a-E1MLS-2xMyc-Ku-IRES-Hygro

   Plasmid

   #234950

   Purpose

   Human codon optimized Ku (Mycobacterium) with N-terminal E1 MLS expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   human codon optimized Ku with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE2-EF1a-E1MLS-2xFLAG-LigD-IRES-tdTomato

   Plasmid

   #234951

   Purpose

   Human codon optimized LigD (Mycobacterium) with N-terminal E1 MLS expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   Human codon optimized LigD with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBOB-CAG-SARS-CoV2-Spike-HA

   Plasmid

   #141347

   Purpose

   3. Generation lentiviral vector expressing the codon optimized SARS-CoV2 Spike Glycoprotein ORF with a C-terminal HA tag generated by Junko Ogawa & Gerald M Pao

   Depositor

   Gerald Pao

   Article

   Ogawa et al bioRxiv. 2020 Jul 22. doi: 10.1101/2020.

   Insert

   SARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   resynthesized with human codon optimization nucle…

   Promoter

   CAG

   Available since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNMSB104

   Plasmid

   #199314

   Purpose

   MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChRmine::SL2::jRGECO1a::let-858 3'UTR at ttTi5605

   Depositor

   Michael Krieg

   Article

   Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

   Insert

   flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChRmine::SL2::jRGECO1a::let-858 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   mTagBFP2 from pJJR81, C. elegans codon optimized …

   Promoter

   flp-18

   Available since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. LeGO-iBSD-5LO-WT

   Plasmid

   #182741

   Purpose

   Lentiviral expression vector for codon-optimized 5-Lipoxygenase (WT) in mamallian cells with blasticidin S resistance (bsd).

   Depositor

   Dieter Steinhilber

   Article

   Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d

   Insert

   5-Lipoxygenase (ALOX5 Human)
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   codon optimized for expression in homo sapiens

   Promoter

   SFFV

   Available since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSH-EFIRES-P-GFP(1-10)opti

   Plasmid

   #129416

   Purpose

   Expressing codon-optimized GFP(1-10) fragment in human cells

   Depositor

   Elina Ikonen

   Article

   Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

   Insert

   codon-optimized GFP(1-10)
   

   Use

   CRISPR, TALEN, and Unspecified ; Human safe harbo…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHL-EF1a-SphcCas9-iP-A

    Plasmid

    #60599

    Purpose

    Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

    Depositor

    Akitsu Hotta

    Article

    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

    Insert

    CRISPR Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Codon usage optimized for human usage

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5-FRT-TO-Nprot-HF_GC3opt

    Plasmid

    #157732

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nprot-HF_GC3opt (N SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pCAG-hFnCas12a-NLS(nucleoplasmin)-3xHA (AAS1472)

    Plasmid

    #114089

    Purpose

    CAG promoter expression plasmid for human codon optimized FnCas12a nuclease with C-terminal NLS and HA tag

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized FnCas12a
    

    Use

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5-FRT-TO-Nsp8-Nsp7-HF_GC3opt

    Plasmid

    #157702

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp8-Nsp7-HF_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pcDNA-FRT-TO-FH-Nsp8-Nsp7_GC3opt

    Plasmid

    #157701

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp8-Nsp7_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pCAG-hMbCas12a-NLS(nucleoplasmin)-3xHA (AAS2134)

    Plasmid

    #114090

    Purpose

    CAG promoter expression plasmid for human codon optimized MbCas12a nuclease with C-terminal NLS and HA tag

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized MbCas12a
    

    Use

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTE3328

    Plasmid

    #107539

    Purpose

    Expresses As crRNA and human codon optimized AsCpf1(RR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    As crRNA
    hAsCpf1(RR mutant)
    

    Use

    Tags

    3xHA, NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    S542R, K607R

    Promoter

    CMV and human U6

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTE3329

    Plasmid

    #107538

    Purpose

    Expresses Lb crRNA and human codon optimized LbCpf1(RR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Lb crRNA
    hLbCpf1(RR mutant)
    

    Use

    Tags

    3xHA, NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    G532R, K595R

    Promoter

    CMV and human U6

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTE3327

    Plasmid

    #107535

    Purpose

    Expresses As crRNA and human codon optimized AsCpf1(RVR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    As crRNA
    hAsCpf1(RVR mutant)
    

    Use

    Tags

    3xHA, NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    S542R, K548V, N552R

    Promoter

    CMV and human U6

    Available since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUCIDT-attL1-Worm ABeta-attR5

    Plasmid

    #160437

    Purpose

    Entry vector for cloning nematode-codon-optimized Amyloid Beta(1-42) using 2-fragment gateway recombination

    Depositor

    Nicholas Tolwinski

    Article

    Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

    Insert

    Homo sapiens amyloid beta precursor protein (APP Nematode, Human)
    

    Use

    Tags

    Cleavage signal

    Expression

    Bacterial

    Mutation

    Codon-optimized for expression in C. elegans

    Promoter

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTE3334

    Plasmid

    #107537

    Purpose

    Expresses Mb crRNA and human codon optimized MbCpf1(RVR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Mb crRNA
    hMbCpf1(RVR mutant)
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    N576R, K582V, N586R

    Promoter

    CMV and human U6

    Available since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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