We narrowed to 6,177 results for: nls

1. pCDH-NLS-copGFP-EF1-BlastiS

   Plasmid

   #132772

   Purpose

   Spills into the cytoplasm during nuclear envelope rupture.

   Depositor

   Jan Lammerding

   Article

   Denais et al Science. 2016 Apr 15;352(6283):353-8. do

   Insert

   NLS-copGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. phage UbiC NLS HA stdMCP stdHalo

   Plasmid

   #104999

   Purpose

   Lentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag for mRNA labeling

   Depositor

   Jeffrey Chao

   Article

   Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

   Insert

   stdMCP-stdHalo
   

   Use

   Lentiviral

   Tags

   HA tag, NLS, Two synonymous copies of the Halo-ta…

   Expression

   Bacterial and Mammalian

   Promoter

   UbiC

   Available Since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CROPseq-multi-EBFP2-NLS (pRW1447)

   Plasmid

   #216219

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi construct with nuclear-localized EBFP2 expression

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCIG-V5-Snail2-IRES-nls-GFP

   Plasmid

   #44282

   Depositor

   Martin Cheung

   Article

   Liu et al Proc Natl Acad Sci U S A. 2013 Feb 19;11

   Insert

   V5-Snail2 (SNAI2 Chicken)
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV-IE-enhancer and chicken beta-actin promoter

   Available Since

   Aug. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AsCas12a-6xNLS-E174R/S542R (pRG232)

   Plasmid

   #149723

   Purpose

   Lentiviral expression plasmid of AsCas12a-6xNLS-E174R/S542R with puromycin resistance gene

   Depositor

   Junwei Shi

   Article

   Gier et al Nat Commun. 2020 Jul 13;11(1):3455. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AAVS1-Puro CAG eGFP NLS

   Plasmid

   #170830

   Purpose

   Donor plasmid for knocking eGFP NLS into AAVS1 safe harbor locus

   Depositor

   Xiaoping Bao

   Article

   Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

   Insert

   eGFP
   

   Use

   CRISPR, Synthetic Biology, and TALEN

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLX_TRC311/NLS-Cas13d-P2A-Blast

   Plasmid

   #212196

   Purpose

   CasRx (NLS-RfxCas13d-NLS) with 2A-Blasticidin for RNA targeting. CasRx-2A-Blasticidin is driven by EF1a promoter. EGFP is driven by SV40

   Depositor

   Stacey Edwards

   Article

   Wang et al Mol Cancer. 2024 May 15;23(1):101. doi:

   Insert

   CasRx (NLS-RfxCas13d-NLS)-HA with 2A-Blasticidin
   

   Use

   CRISPR and Lentiviral

   Tags

   HA, NLS, and blasticidin S deaminase

   Expression

   Mammalian

   Promoter

   EF-1α promoter

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. phage ubc nls ha pcp gfp

   Plasmid

   #64539

   Purpose

   Used to label reporter mRNAs. Lentiviral expression of the PP7 coat protein fused to EGFP.

   Depositor

   Jeffrey Chao

   Article

   Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

   Insert

   PCP
   

   Use

   Lentiviral

   Tags

   EGFP, FactorXa site, HA, and NLS

   Expression

   Mammalian

   Promoter

   human ubiquitin C promoter

   Available Since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. NLS-Laconic/pCMV-myc-nuc

   Plasmid

   #46307

   Purpose

   a nuclear-targeted, genetically-encoded sensor for lactate

   Depositor

   Luis Felipe Barros

   Article

   Barros et al Front Cell Neurosci. 2013;7:27. doi: 10.

   Insert

   NLS-Laconic
   

   Use

   Lactate biosensor

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28-SpRY-NLS-6xHis (gbr2101)

    Plasmid

    #181743

    Purpose

    bacterial expression plasmid for SpRY with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Insert

    bacterial codon optimized SpRY
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS(SV40)-6xHis and MKIEE MBP tag

    Expression

    Bacterial

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

    Promoter

    T7

    Available Since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. phage-ubc-nls-ha-tdMCP-gfp

    Plasmid

    #40649

    Depositor

    Robert Singer

    Article

    Wu et al Biophys J. 2012 Jun 20;102(12):2936-44.

    Insert

    tdMCP-gfp
    

    Use

    Lentiviral

    Tags

    EGFP, HA, and NLS

    Expression

    Mammalian

    Promoter

    human ubiquitin C (UBC)

    Available Since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. phage-ubc-nls-ha-tdPCP-gfp

    Plasmid

    #40650

    Depositor

    Robert Singer

    Article

    Wu et al Biophys J. 2012 Jun 20;102(12):2936-44.

    Insert

    tdPCP-gfp
    

    Use

    Lentiviral

    Tags

    EGFP, HA, and NLS

    Expression

    Mammalian

    Promoter

    human ubiquitin C (UBC)

    Available Since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDNA3.1 (-) + FLAG-NLS-MS2-eGFP

    Plasmid

    #86827

    Purpose

    Expresses a nuclear localized, FLAG-tagged MS2-GFP fusion protein in mammalian cells, used for purification or localization of tagged RNAs

    Depositor

    Carl Novina

    Article

    Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

    Insert

    FLAG-NLS-MS2-eGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2017

    Availability

    Academic Institutions and Nonprofits only

14. pENTR_L1-miniTurbo-YFP-NLS-STOP-L2

    Plasmid

    #127362

    Purpose

    gateway entry vector for expressing nuclear miniTurbo-YFP under a promoter of choice

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NLS, V5, and YFP

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. hGFAP-NLS-HA-Dre-P2A-BFP

    Plasmid

    #51277

    Purpose

    Co-expression of the site-specific recombinase Dre and BFP under the control of the hGFAP promoter

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    NLS-HA-Dre-P2A-BFP
    

    Expression

    Mammalian

    Promoter

    hGFAP

    Available Since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pL6puro-pmRevErbA-Venus-NLS-Pest

    Plasmid

    #240105

    Purpose

    A lentiviral (yellow) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein)

    Depositor

    Achim Kramer

    Article

    Gabriel et al bioRxiv 2025.06.30.661926

    Insert

    murine Nr1d1 promoter+Venus-NLS-Pest-Reporter (Nr1d1 Mouse)
    

    Use

    Lentiviral

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-NLS-iPASnFR-H-mScarlet3-3xFLAG

    Plasmid

    #225245

    Purpose

    Mammalian expression of iPASnFR-H in the nucleus

    Depositor

    Loren Looger

    Article

    Tamura et al bioRxiv 2024.08.21.609037

    Insert

    iPASnFR-H
    

    Tags

    3xFLAG and mScarlet3

    Expression

    Mammalian

    Available Since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMSCV-NLS-CBGreen-FIRE-puro

    Plasmid

    #139195

    Purpose

    Luminescent reporter of ERK activity

    Depositor

    Matthew Lazzara

    Article

    Day et al Cell Rep. 2020 Mar 10;30(10):3383-3396.e

    Insert

    CBG99
    

    Use

    Luciferase and Retroviral

    Tags

    Fra1 PEST domain fragment (FIRE) and nuclear loca…

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-NLS-iPASnFR-L-mScarlet3-3xFLAG

    Plasmid

    #225246

    Purpose

    Mammalian expression of iPASnFR-L in the nucleus

    Depositor

    Loren Looger

    Article

    Tamura et al bioRxiv 2024.08.21.609037

    Insert

    iPASnFR-L
    

    Tags

    3xFLAG and mScarlet3

    Expression

    Mammalian

    Available Since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PET-B-Sniper SpCas9-NLS-6xHis

    Plasmid

    #207377

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-Sniper SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    F539S, M763I, K890N, amino acids 1005-1013 replac…

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge