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pCMVMagFRET-1-NLS

Plasmid

#50748

Purpose

Expresses MagFRET-1 in the nucleus of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal Ca2+ levels.

Depositor

Maarten Merkx

Article

Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

Insert

gene encoding for MagFRET-1 with NLS

Expression

Mammalian

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pET_RFP-SZ2

Plasmid

#124933

Purpose

Expression of RFP-SZ2

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RFP-SYNZIP2

Tags

His and mCherry

Expression

Bacterial

Mutation

SZ2 domain

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pECNUS_MP02

Plasmid

#194351

Purpose

CRISPR-Cas9 multiplexing acceptor clone with BsaI cutter

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

pcoCas9

Expression

Plant

Promoter

RPS5A

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pJB007

Plasmid

#98995

Purpose

Express FtsI tagged with tagRFP-t in E coli

Depositor

Jie Xiao

Article

Yang et al Science. 2017 Feb 17;355(6326):744-747.

Insert

FtsI

Tags

TagRFP-T

Expression

Bacterial

Promoter

T5-lac

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pU6-cGAS g3 Cas9-T2A-mCherry

Plasmid

#164254

Purpose

Transcription of cGAS guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

sgcGAS

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b+H2B

Plasmid

#157809

Purpose

Expression of human histone H2B

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

H2B

Expression

Bacterial

Promoter

T7

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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3xHA-BioID_pAS28

Plasmid

#118223

Purpose

expresses 3xHA-tagged BioID in C. elegans intestine

Depositor

Jessica Feldman

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

BioID (BirA mutant)

Tags

3x HA tag

Expression

Worm

Mutation

R118G

Promoter

ges-1p

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Muc2D1D2D3cysD1

Plasmid

#155214

Purpose

Expresses human MUC2 residues 21-1397

Depositor

Deborah Fass

Article

Javitt et al Cell. 2020 Oct 29;183(3):717-729.e16. do

Insert

Muc2 D1D2D3cysD1 (MUC2 Human)

Tags

6xHistadine

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pBAC-DsRed-QF2-hsp70

Plasmid

#104876

Purpose

General purpose vector for cloning enhancer/promoter regions to drive QF2 expression; used for mosquito

Depositor

Christopher Potter

Article

Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

Insert

QF2

Expression

Insect

Available Since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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CDC42

Plasmid

#55693

Purpose

expression of mouse CDC42

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

CDC42

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA_RFP-SZ2

Plasmid

#124936

Purpose

Expression of RFP-SZ2

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RFP-SYNZIP2

Tags

mCherry

Expression

Mammalian

Mutation

SZ2 domain

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pOCC82

Plasmid

#118862

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for untagged recombinant protein

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-NotI-ccdB-AscI-stop-HindIII cassette

Expression

Insect

Promoter

polH

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pET28-OmpT

Plasmid

#68862

Purpose

construct for expression of OmpT protease in the pET28a plasmid using the restriction sites NcoI and EcoRI.

Depositor

Neil Kelleher

Article

Wu et al Nat Methods. 2012 Jun 17;9(8):822-4. doi

Insert

OmpT (ompT )

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pSG5-p110α-WT

Plasmid

#55709

Purpose

eukaryotic expression of Flag tagged p110a WT

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

p110 alpha

Tags

flag

Expression

Mammalian

Promoter

T7

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pALPS

Plasmid

#90996

Purpose

Lentiviral vector for expression of heterologous genes

Depositor

Jeremy Luban

Article

Pertel et al Nature. 2011 Apr 21;472(7343):361-5. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-K20

Plasmid

#14743

Depositor

M Bishr Omary

Article

Zhou et al Mol Biol Cell. 2003 Jul . 14(7):2959-71.

Insert

Keratin 20 (KRT20 Human)

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pBFv-UAS3-GFP1-10

Plasmid

#138398

Purpose

Expresses split GFP fragment (GFP1-10)

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

GFP1-10

Expression

Insect

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-TREX1 g2 Cas9-T2A-mCherry

Plasmid

#164251

Purpose

Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

sgTREX1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pCMB-TPr

Plasmid

#61190

Purpose

Fluorescent reporter for tonoplast AtTIP1-1-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtTIP1-1

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

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pET15bLCFPT7

Plasmid

#53545

Purpose

Activation module. The plasmid consists of a mutant T7 RNA polymerase (T7 RNAP) activating its own expression via a T7 promoter carrying alac operator. Carbenicillin resistant

Depositor

Lingchong You

Article

Payne et al Mol Syst Biol. 2013 Oct 8;9:697. doi: 10

Insert

T7 RNAP

Expression

Bacterial

Available Since

July 28, 2014

Availability

Academic Institutions and Nonprofits only

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