We narrowed to 5,516 results for: pcas

1. pCAFNF-ECFP-Actb

   Plasmid

   #169787

   Purpose

   An actin FRET sensor. Must be expressed with Venus-actin for FRET.

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Actb (Actb Mouse)
   

   Use

   Tags

   ECFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-Venus-MDGA1

   Plasmid

   #82543

   Purpose

   Overexpression vector for Venus-MDGA1 in neurons

   Depositor

   Alice Ting

   Article

   Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

   Insert

   MDGA1 (MDGA1 Human)
   

   Use

   Tags

   Venus fluorescent protein

   Expression

   Mammalian

   Mutation

   Promoter

   chicken beta actin

   Available since

   Sept. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-Izumo1-1D4

   Plasmid

   #183544

   Purpose

   Expression vector of mouse izumo sperm-egg fusion 1 (Izumo1) tagged with 1D4 at C-terminus.

   Depositor

   Masahito Ikawa

   Article

   Noda et al Commun Biol. 2022 Apr 7;5(1):332. doi: 1

   Insert

   izumo sperm-egg fusion 1 (Izumo1 Mouse)
   

   Use

   Tags

   1D4

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-Ace2_D92N_E199V-mRuby3

   Plasmid

   #129257

   Purpose

   Express Ace2_D92N_E199V-mRuby3 in mammalian cells

   Depositor

   Eric Schreiter

   Article

   Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

   Insert

   Ace2_D92N_E199V-mRuby3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D92N, E199V

   Promoter

   CAG

   Available since

   Aug. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-AaCas12b-2AeGFP

   Plasmid

   #121945

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

   Insert

   AaCas12b
   

   Use

   Tags

   3xHA tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-rTetRDBD-GBP1

   Plasmid

   #50791

   Purpose

   Expression of a GFP-dependent transcription factor component in mammalian cells

   Depositor

   Connie Cepko

   Article

   Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

   Insert

   rTetR DNA binding domain-GBP1 fusion protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL3-B-161;+80pCalb2

   Plasmid

   #66745

   Purpose

   luciferase reporter for Calb2 promoter (-161to +80, WT)

   Depositor

   Rolf Stahel, Emanuela Felley-Bosco

   Article

   Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

   Insert

   Calb2 promoter (-161bp+80) (CALB2 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Calb2

   Available since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-C1V1-TT-mRuby2-ST

   Plasmid

   #109124

   Purpose

   Cation channelrhodopsin C1V1 fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

   Depositor

   Hillel Adesnik

   Article

   Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

   Insert

   C1V1-TT-mRuby2-ST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTrex-b-NLS-hSpCas9

   Plasmid

   #62543

   Purpose

   Expression of Cas9 in T. cruzi

   Depositor

   Rick Tarleton

   Article

   Peng et al MBio. 2014 Dec 30;6(1). pii: e02097-14.

   Insert

   NLS-hSpCas9
   

   Use

   CRISPR; Trypanosoma cruzi expression

   Tags

   3xFLAG and NLS

   Expression

   Mutation

   Promoter

   Ribo-HX1

   Available since

   April 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-Cre-iBSD

    Plasmid

    #140284

    Purpose

    Expression of Cre-IRES-BSD

    Depositor

    Yusuke Miyanari

    Article

    Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

    Insert

    Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330_Actb sgRNA / hSpCas9

    Plasmid

    #172832

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1of Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 1 of Actb under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpCas9(BB)-2A-GFP-G3BP2(1)

    Plasmid

    #136060

    Purpose

    G3BP2 gRNA (#1) inserted into the pSpCas9(BB)-2A-GFP plasmid (TCATACTAAAATTCGTCATG)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP2 (G3BP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. G1333 DddAtox-N–dSpCas9

    Plasmid

    #157833

    Purpose

    expresses split DddAtox-Cas9 construct in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    bpNLS–G1333 DddAtox-N–dSpCas9–UGI–UGI–bpNLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-GBP7-p65AD

    Plasmid

    #49441

    Purpose

    Expression of a GFP-dependent transcription factor component in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    GBP7-p65 transactivation domain fusion protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMA-SpCas9-g10

    Plasmid

    #80793

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 10/20/30 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGL3-B-264;+80pCalb2

    Plasmid

    #66746

    Purpose

    luciferase reporter for Calb2 promoter (-264 to +80, WT)

    Depositor

    Rolf Stahel, Emanuela Felley-Bosco

    Article

    Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

    Insert

    Calb2 promoter (-264bp+80) (CALB2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Calb2

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGL3-B-419;+80pCalb2

    Plasmid

    #66747

    Purpose

    luciferase reporter for Calb2 promoter (-419 to +80, WT)

    Depositor

    Rolf Stahel, Emanuela Felley-Bosco

    Article

    Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

    Insert

    Calb2 promoter (-419bp+80) (CALB2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Calb2

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330-NL-DHFR-SpCas9

    Plasmid

    #124522

    Purpose

    Expresses SpCas9 fused to DHFR domains on both the N-terminus and an internal loop in mammalian cells

    Depositor

    Amit Choudhary

    Article

    Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

    Insert

    NL-DHFR-SpCas9
    

    Use

    AAV

    Tags

    Destabilized domain of E.coli dihydrofolate reduc…

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-Venus-MDGA2

    Plasmid

    #82544

    Purpose

    Overexpression vector for Venus-MDGA2 in neurons

    Depositor

    Alice Ting

    Article

    Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

    Insert

    MDGA2 (Mdga2 Rat)
    

    Use

    Tags

    Venus fluorescent protein

    Expression

    Mammalian

    Mutation

    Promoter

    chicken beta actin

    Available since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-hPPP3CB-FLAG

    Plasmid

    #179135

    Purpose

    Expression vector of human protein phosphatase 3, catalytic subunit, beta isoform (PPP3CB) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

    Insert

    protein phosphatase 3, catalytic subunit, beta isoform (PPP3CB Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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