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VN1897 pB2Hws_v28_Ddcm_wkKanR_TIR51_pLWeakRBS_TIR371_cI-Asf1B+rpoA-2GG

Plasmid

#235119

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B. In fusion cloning to rpoA using BsaI based golden gate.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Type

Empty backbone

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P

Expression

Bacterial

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(R5A)

Plasmid

#245193

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 4:2025.07.0

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

R5A

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(R4A,R5A)

Plasmid

#245194

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 4:2025.07.0

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

R4A,R5A

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(R4A)

Plasmid

#245192

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 4:2025.07.0

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

R4A

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(E139A)

Plasmid

#245207

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 4:2025.07.0

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

E139A

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-empty

Plasmid

#239099

Purpose

pGL3 luciferase vector with no promoter.

Depositor

David Cobrinik

Article

Shayler et al eLife 13:RP101918

Type

Empty backbone

Use

Luciferase

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA4-C-EF1α

Plasmid

#239097

Purpose

Empty pcDNA4-C backbone with EF1α promoter.

Depositor

David Cobrinik

Article

Shayler et al eLife 13:RP101918

Type

Empty backbone

Expression

Mammalian

Promoter

EF1α

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pVG329

Plasmid

#227771

Purpose

For genomic replacement of YOR1 gene in budding yeast

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

Genomic sequences flanking YOR1 in budding yeast (YOR1 Budding Yeast)

Expression

Yeast

Mutation

N/A

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pVG178

Plasmid

#227761

Purpose

Expresses a version of light-inducible transcription factor EL222 (Glu84Lys) under a strong constitutive PGK1pr

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

EL222 codon-optimized for budding yeast

Expression

Yeast

Mutation

Glu84KLys mutant

Promoter

PGK1pr

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pVG144

Plasmid

#227760

Purpose

Expresses a version of light-inducible transcription factor EL222 (Val121Met) under a strong constitutive PGK1pr

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

EL222 codon-optimized for budding yeast

Expression

Yeast

Mutation

Val121Met mutant

Promoter

PGK1pr

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pVG150

Plasmid

#227759

Purpose

Expresses a version of light-inducible transcription factor EL222 (Ser137Asn) under a strong constitutive PGK1pr

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

EL222 codon-optimized for budding yeast

Expression

Yeast

Mutation

Ser137Asn mutant

Promoter

PGK1pr

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pVG124

Plasmid

#227758

Purpose

Expresses a version of light-inducible transcription factor EL222 (Thr83Ser) under a strong constitutive PGK1pr

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

EL222 codon-optimized for budding yeast

Expression

Yeast

Mutation

Thr83Ser mutant

Promoter

PGK1pr

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pVG121

Plasmid

#227757

Purpose

Expresses a version of light-inducible transcription factor EL222 (Gly82Val) under a strong constitutive PGK1pr

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

EL222 codon-optimized for budding yeast

Expression

Yeast

Mutation

Gly80Glu mutant

Promoter

PGK1pr

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pVG123

Plasmid

#227755

Purpose

Expresses a version of light-inducible transcription factor EL222 (Ala157Val) under a strong constitutive PGK1pr

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

EL222 codon-optimized for budding yeast

Expression

Yeast

Mutation

Ala157Val mutant

Promoter

PGK1pr

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pVG143

Plasmid

#227754

Purpose

Expresses a version of light-inducible transcription factor EL222 (Leu87Ile) under a strong constitutive PGK1pr

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

EL222 codon-optimized for budding yeast

Expression

Yeast

Mutation

Leu87Ile

Promoter

PGK1pr

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pVG147

Plasmid

#227753

Purpose

Expresses a version of light-inducible transcription factor EL222 (Gly94Cys) under a strong constitutive PGK1pr

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

EL222 codon-optimized for budding yeast

Expression

Yeast

Mutation

Gly94Cys mutant

Promoter

PGK1pr

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pVG120

Plasmid

#227752

Purpose

Expresses a version of light-inducible transcription factor EL222 (Asp89Tyr) under a strong constitutive PGK1pr

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

EL222 codon-optimized for budding yeast

Expression

Yeast

Mutation

Asp89Tyr mutant

Promoter

PGK1pr

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pVG137

Plasmid

#227751

Purpose

Expresses a version of light-inducible transcription factor EL222 (Val201Leu) under a strong constitutive PGK1pr

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

EL222 codon-optimized for budding yeast

Expression

Yeast

Mutation

Val201Leu mutant

Promoter

PGK1pr

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pVG136

Plasmid

#227750

Purpose

Expresses a version of light-inducible transcription factor EL222 (Pro165Ser) under a strong constitutive PGK1pr

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

EL222 codon-optimized for budding yeast

Expression

Yeast

Mutation

Pro165Ser mutant

Promoter

PGK1pr

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pVG135

Plasmid

#227749

Purpose

Expresses a version of light-inducible transcription factor EL222 (Lys99Glu) under a strong constitutive PGK1pr

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Cell. 2026 Mar 6:S0092-8674(26)00167-4.

Insert

EL222 codon-optimized for budding yeast

Expression

Yeast

Mutation

Lys99Glu mutant

Promoter

PGK1pr

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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