Addgene

We narrowed to 2,052 results for: 3-Oct

Showing: 1201 - 1220 of 2052 results

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cASIC1 concatemer - fully assembled

Plasmid

#233133

Purpose

To create cASIC1 channels of defined stoichiometry in mammalian cells

Depositor

David MacLean

Article

Purohit et al Biophys J. 2024 Oct 15;123(20):3507-3518

Insert

ASIC1 (ASIC1 Chicken)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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cASIC1 concatemer - Subunit C

Plasmid

#233131

Purpose

To create cASIC1 channels of defined stoichiometry in mammalian cells

Depositor

David MacLean

Article

Purohit et al Biophys J. 2024 Oct 15;123(20):3507-3518

Insert

ASIC1 (ASIC1 Chicken)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
cASIC1 concatemer - Subunit B

Plasmid

#233130

Purpose

To create cASIC1 channels of defined stoichiometry in mammalian cells

Depositor

David MacLean

Article

Purohit et al Biophys J. 2024 Oct 15;123(20):3507-3518

Insert

ASIC1 (ASIC1 Chicken)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-FLAG-APEX2-NES-HIS3

Plasmid

#229230

Purpose

Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

Depositor

Fulvio Reggiori

Article

Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-FLAG-APEX2-NES-K.l.LEU2

Plasmid

#229229

Purpose

Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

Depositor

Fulvio Reggiori

Article

Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-FLAG-APEX2-NES-NatMX6

Plasmid

#229231

Purpose

Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

Depositor

Fulvio Reggiori

Article

Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-FLAG-APEX2-NES-TRP1

Plasmid

#229228

Purpose

Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

Depositor

Fulvio Reggiori

Article

Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pET26b(modified)-Lg-B

Plasmid

#216960

Purpose

Expression of Large BiT-Linker-GvpB

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Large BiT-Linker-GvpB

Use

Tags

FLAG-Split Nanoluciferase LargeBiT

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b(modified)-B-Lg

Plasmid

#216959

Purpose

Expression of GvpB-Linker-Large BiT

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

GvpB-Linker-Large BiT

Use

Tags

Split Nanoluciferase LargeBiT-FLAG

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pST39-Mega'

Plasmid

#216877

Purpose

Derived from the plasmid for Mega GVs, pST39-pNL29 (Addgene #91696), but the overlap between GvpS and GvpK has been resolved.

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Derived from Mega, and GvpS and GvpK overlap has been resolved

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pJFRC7-JEDI-1P

Plasmid

#202618

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-1P under the promoter hsp70 for Drosophila (insect) expression

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P

Use

Tags

Expression

Insect

Mutation

Promoter

hsp70

Available since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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pBa.Halo-KAP3B

Plasmid

#193940

Purpose

Mammalian expression of Halo-tagged KAP 3B

Depositor

Marvin Bentley

Article

Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

Insert

KAP 3B (KIFAP3 Human)

Use

Tags

Halo

Expression

Mammalian

Mutation

Promoter

Chicken Beta actin

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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CC0101 BFPmut-stop-Bdnf.t1 (pEAQ)

Plasmid

#191173

Purpose

An orthogonal trigger sequence used in plant experiments, derived from the 3' UTR of murine Bdnf

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

Bdnf.t1 (plant)

Use

Tags

Expression

Plant

Mutation

WT

Promoter

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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EK0441 CMV-TO-BFPmut-stop-EGFP.t2 (FLP-IN)

Plasmid

#191161

Purpose

Expression of a trigger sequence from EGFP CDS in 3' UTR context in mammalian cells

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

EGFP.t2

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-DiB1

Plasmid

#168800

Purpose

Fluorogen activating protein (FAP) DiB1

Depositor

Alexander Mishin

Article

Bozhanova et al Chem Sci. 2017 Oct 1;8(10):7138-7142. do

Insert

Fluorogen activating protein DiB1

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD-DiB3

Plasmid

#168802

Purpose

Fluorogen activating protein (FAP) DiB3

Depositor

Alexander Mishin

Article

Bozhanova et al Chem Sci. 2017 Oct 1;8(10):7138-7142. do

Insert

Fluorogen activating protein DiB3

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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OfCasp3b

Plasmid

#166054

Purpose

a caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but function similar to caspase-6

Depositor

Clay Clark

Article

Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1

Insert

Orbicella faveolata caspase-3b

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7 lac promoter

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pGS-3R112C/T180C

Plasmid

#85095

Purpose

Host vector to fuse a gene at the N-terminus of PCNA3 variant (R112C/T180C)

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

DNA polymerase sliding clamp 3

Use

Tags

His6 tag

Expression

Bacterial

Mutation

changed Arginine 112 to Cysteine and Threonine 18…

Promoter

T7 promoter

Available since

Nov. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Dlx4

Plasmid

#74343

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Dlx4. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

distal-less homeobox 4 (Dlx4 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

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pBGSA FPR1 Q258A

Plasmid

#66724

Purpose

Constitutive expression of FPR1 Q258A in mammalian cells.

Depositor

Heini Miettinen

Article

Miettinen et al J Immunol. 1997 Oct 15;159(8):4045-54.

Insert

FPR1 (haplotype 3) (FPR1 Human)

Use

Tags

Expression

Mammalian

Mutation

Q258A

Promoter

SR-alpha

Available since

June 23, 2015

Availability

Academic Institutions and Nonprofits only