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Showing: 1201 - 1220 of 8351 results

  1. pKEK3237

    Plasmid
    #234091
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    sfGFP
    Use
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    S30R/Y39N/N105T/Y145F/I171V/A206V
    Promoter
    pJ23100-BsaI
    Available since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    234091-plasmid-map-sequence-id-467987

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  2. pKEK3400

    Plasmid
    #234084
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    sfGFP
    Use
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    S30R/Y39N/N105T/Y145F/I171V/A206V
    Promoter
    pJ23100-BsaI
    Available since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234084-plasmid-map-sequence-id-464936

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  4. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB3

    Plasmid
    #201534
    Purpose
    Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)
    Depositor
    Dan Park
    Article
    Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d
    Insert
    ilvA-relE_RBS3_CB3
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    rhaS W190G
    Promoter
    PrhaBAD (rhamnose)
    Available since
    Sept. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    201534-plasmid-map-sequence-id-406801

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  6. IBMc180

    Plasmid
    #161942
    Purpose
    Carries Golden Gate substitution insert for acVHH-assisted bisection mapping
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    161942-plasmid-map-sequence-id-329042

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  10. pJK381

    Plasmid
    #71573
    Purpose
    Produces Thermoplasma acidophilum citrate synthase, Asp317>Gly mutant (TpCS-D317G)
    Depositor
    T. J. Kappock
    Article
    Kurz et al Biochemistry. 2009 Aug 25;48(33):7878-91
    Insert
    citrate synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes aspartate-317 to glycine
    Promoter
    recA
    Available since
    April 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    71573-plasmid-map-sequence-id-140454

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  11. pJK382

    Plasmid
    #71574
    Purpose
    Produces Thermoplasma acidophilum citrate synthase, Asp317>Asn mutant (TpCS-D317N)
    Depositor
    T. J. Kappock
    Article
    Kurz et al Biochemistry. 2009 Aug 25;48(33):7878-91
    Insert
    citrate synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes aspartate-317 to asparagine; silent mutat…
    Promoter
    recA
    Available since
    March 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    71574-plasmid-map-sequence-id-130942

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  13. pJK360

    Plasmid
    #71571
    Purpose
    Produces Acetobacter aceti 1023 acetyl-CoA:oxalate CoA-transferase (UctC) with Thr2>Ala mutant (UctC-T2A)
    Depositor
    T. J. Kappock
    Article
    Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d
    Insert
    acetyl-CoA:oxalate CoA-transferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes threonine-2 to alanine
    Promoter
    T7
    Available since
    Jan. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71571-plasmid-map-sequence-id-130928

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  14. pJK385

    Plasmid
    #71572
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag (AarCH6)
    Depositor
    T. J. Kappock
    Article
    Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d
    Insert
    succinyl-CoA:acetate CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71572-plasmid-map-sequence-id-130931

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  15. pJK347

    Plasmid
    #71833
    Purpose
    Cloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE(H59D), and purK genes
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to aspartate
    Promoter
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    71833-plasmid-map-sequence-id-131050

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  16. pJK348

    Plasmid
    #71832
    Purpose
    Cloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE(H59N), and purK genes
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to asparagine
    Promoter
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    71832-plasmid-map-sequence-id-131051

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  17. pJK193

    Plasmid
    #71834
    Purpose
    Cloned region contains Acetobacter aceti 1023 rpmE-radC(and beyond) region
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Phage lambda cloning vector with genomic dna inse…
    Tags
    Expression
    Mutation
    additional N-terminal methionine encoded by this …
    Promoter
    Available since
    Jan. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71834-plasmid-map-sequence-id-131053

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  18. pGS1112

    Plasmid
    #63784
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eSapphire Strep-tag II followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eSapphire-Strep-tag II followed by URA3
    Use
    E. coli cloning vector
    Tags
    Sapphire-Strep-tag II
    Expression
    Mutation
    Promoter
    none
    Available since
    April 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    63784-plasmid-map-sequence-id-106474

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Showing: 1201 - 1220 of 8351 results