We narrowed to 8,805 results for: App

1. pJK281

   Plasmid

   #71137

   Purpose

   Expression of Acetobacter aceti 1023 PurE (H59F mutant) in E. coli

   Depositor

   T. J. Kappock

   Article

   Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Expression

   Bacterial

   Mutation

   changes histidine-59 to phenylalanine; additional…

   Promoter

   T7

   Available Since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJK280

   Plasmid

   #71136

   Purpose

   Expression of Acetobacter aceti 1023 PurE (H89F mutant) in E. coli

   Depositor

   T. J. Kappock

   Article

   Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Expression

   Bacterial

   Mutation

   changes histidine-89 to phenylalanine; additional…

   Promoter

   T7

   Available Since

   Jan. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK175

   Plasmid

   #71132

   Purpose

   Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase (class I PurE) in E. coli

   Depositor

   T. J. Kappock

   Article

   Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Expression

   Bacterial

   Mutation

   additional N-terminal methionine encoded by this …

   Promoter

   T7

   Available Since

   Jan. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGMCKq20-T38S38-0X

   Plasmid

   #49522

   Purpose

   Kanamycin resistant; contains qtag20 and constitutively expresses reverse TetR (tetR38); integrates into the mycobacteriophage att-L5 site

   Depositor

   Dirk Schnappinger

   Article

   Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

   Insert

   constitutive TetR38
   

   Expression

   Bacterial

   Promoter

   Ptb38

   Available Since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. 4xHRE-MinTK-CRE-ODD

   Plasmid

   #141147

   Purpose

   lentiviral expression vector to generate a hypoxia fate-mapping system

   Depositor

   Daniele Gilkes

   Article

   Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

   Insert

   4xHRE-MinTK-CRE-ODD
   

   Use

   Lentiviral

   Mutation

   contains an altered Cre gene modified by the addi…

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV-loxp-DsRed-loxp-eGFP

   Plasmid

   #141148

   Purpose

   lentiviral expression vector to generate a hypoxia fate-mapping system

   Depositor

   Daniele Gilkes

   Article

   Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

   Insert

   loxp-DsRed-loxp-eGFP
   

   Use

   Lentiviral

   Available Since

   Sept. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. IgK-IFN-luc

   Plasmid

   #14886

   Purpose

   Reporter construct for NF‐κB activation. Contains two copies of immunoglobulin kappa light chain motif (5′‐GGGGACTTTCC‐3′) upstream of the a minimal promoter driving luciferase expression

   Depositor

   David Baltimore

   Article

   Pomerantz et al EMBO J. 2002 Oct 1. 21(19):5184-94.

   Insert

   IgK-IFN (IFNB1 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   May 1, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-hDcp2

   Plasmid

   #72214

   Purpose

   bacterial expression of His-tagged human Dcp2

   Depositor

   Megerditch Kiledjian

   Article

   Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

   Insert

   Dcp2 (DCP2 Human)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pET28b-His-hCE

   Plasmid

   #112710

   Purpose

   Expresses N-terminally His-tagged human CE/RNGTT in bacterial cells

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

   Insert

   RNGTT (RNGTT Human)
   

   Tags

   6xHis and Stop codons placed after RNGTT sequence…

   Expression

   Bacterial

   Mutation

   R594H

   Promoter

   T7

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGMCtKq28-TSC10M1-sspB

    Plasmid

    #49526

    Purpose

    Kanamycin resistant; contains qtag28, expresses sspB under a promoter containing PtetO, and constutively expresses single-chain TetR10 (tsc10); integrates into the mycobacteriophage att-Tweety site

    Depositor

    Dirk Schnappinger

    Article

    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

    Insert

    TSC10M1-sspB
    

    Expression

    Bacterial

    Available Since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGMCtN-TSC10M1-sspB

    Plasmid

    #49521

    Purpose

    Nourseothricin resistant; expresses sspB under the control of a promoter containing PtetO and constitutively expressses single-chain TetR10 (tsc10); integrates into mycobacteriophage att-Tweety site

    Depositor

    Dirk Schnappinger

    Article

    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

    Insert

    TSC10M1-sspB
    

    Available Since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-myc NES-mCE NLS

    Plasmid

    #82466

    Purpose

    Expresses cytoplasmic restricted form of myc tagged mRNA capping enzyme

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    NES mRNA capping enzyme (Rngtt Mouse, HIV)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJEC726

    Plasmid

    #190814

    Purpose

    CRISPRi backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.

    Depositor

    James Chappell

    Article

    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    dCas9
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    S. pyogenes dCas9 codon optimized for Streptomyce…

    Promoter

    SP30

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJEC819

    Plasmid

    #190815

    Purpose

    CRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.

    Depositor

    James Chappell

    Article

    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    dCas9-XTEN-rpoA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    XTEN (linker), followed by the N-terminal domain …

    Promoter

    SP30

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMX-mTAF6

    Plasmid

    #44337

    Depositor

    Marc Timmers

    Article

    Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

    Insert

    TAF6
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-eDIO-EYFP

    Plasmid

    #104388

    Purpose

    PiggyBac plasmid encoding EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.

    Depositor

    Volker Busskamp

    Article

    Klapper et al Sci Rep. 2017 Oct 31;7(1):14450. doi: 10

    Insert

    Enhanced Yellow Fluorescent Protein
    

    Use

    Cre/Lox; Piggybac

    Expression

    Mammalian

    Available Since

    Jan. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Sf-U7snRNA-ESE-AS-NsiI

    Plasmid

    #190694

    Purpose

    Basic vector used for the expression of U7-based antisense oligonucleotides

    Depositor

    Pim Pijnappel

    Article

    van der Wal et al Mol Ther Nucleic Acids. 2017 Jun 16;7:90

    Insert

    U7 snRNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U7 promoter

    Available Since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG--uMASS_LF

    Plasmid

    #209763

    Purpose

    Expression of uMASS_LF (loss-of-function) in mammalian cells under CAG promoter

    Depositor

    Andre Berndt

    Article

    Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

    Insert

    uMASS_LF
    

    Expression

    Mammalian

    Available Since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-uMASS_1

    Plasmid

    #209762

    Purpose

    Expression of uMASS_1 in mammalian cells under CAG promoter

    Depositor

    Andre Berndt

    Article

    Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

    Insert

    uMASS_1
    

    Expression

    Mammalian

    Available Since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMX-hTAF7

    Plasmid

    #44338

    Depositor

    Marc Timmers

    Article

    Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

    Insert

    TAF7 (TAF7 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    May 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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