We narrowed to 2,903 results for: BFP

1. LV anti-FITC-LaG16-R103A-T2A-BFP

   Plasmid

   #194935

   Purpose

   LV construct encoding R103A mutant sNRR-based SynNotch receptor

   Depositor

   John Ngo

   Article

   Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

   Insert

   antiFITC-LaG16-sNRR(R103A)-SynNotch-Gal4-VP64-T2A-BFP
   

   Use

   Lentiviral and Synthetic Biology

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Ef1a-DIO-hBFP-2A-RVG-WPRE

   Plasmid

   #120268

   Purpose

   Permits retrograde transsynaptic spread by driving Cre-dependent co-expression of the RbG plus a nuclear reporter

   Depositor

   Edward Callaway

   Article

   Faget et al Cell Rep. 2016 Jun 21;15(12):2796-808. d

   Insert

   EF1α-DIO-H2B-tagBFP-Flagx3-T2Am-cB19G
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-mir144 EF1Alpha-puro-T2A-BFP

   Plasmid

   #164791

   Purpose

   Express miR-144 under the U6 promoter in mammalian cells

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   miR-144 (MIR144 Human)
   puro-T2A-BFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1Alpha and U6

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCCL-PGK-SPdCas9-BFP-DNMT3A(E752A)

   Plasmid

   #71213

   Purpose

   dCas9 fused to BFP and the human DNMT3A catalytically inactive domain

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-BFP-DNMT3A(E752A), DNMT3A catalytic domain with inactivation mutation E752A
   

   Tags

   dSpCas9-BFP-DNMT3A(E752A)

   Expression

   Mammalian

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Ef1a-Con/Foff 2.0-BFP

   Plasmid

   #137130

   Purpose

   Intersectional viral expression of BFP in cells expressing Cre AND NOT Flp

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Foff 2.0-BFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   n/a

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTRIP-SFFV-mtagBFP-2A STING V155M 342STOP

   Plasmid

   #102594

   Purpose

   Lentivector to express STING V155M delta 342 and mTagBFP

   Depositor

   Nicolas Manel

   Article

   Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

   Insert

   STING (STING1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   V155M delta 342

   Promoter

   SFFV

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1

   Plasmid

   #164800

   Purpose

   For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   ERH CRISPRi gRNA1
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1Alpha and U6

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2

   Plasmid

   #164801

   Purpose

   For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   ERH CRISPRi gRNA2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1Alpha and U6

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. MADR pDonor TagBFP2-3XFlag (cyto) WPRE

   Plasmid

   #129421

   Purpose

   MADR donor plasmid for FlpO+Cre-mediated insertion of a TagBFP2-3XFlag cytoplasmic reporter protein

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   TagBFP2-3Flag
   

   Use

   Mosaic analysis for dual recombinase-mediated cas…

   Tags

   3X Flag epitope tag

   Promoter

   none (3X polyA to mitigate episomal expression)

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHBS1504 [IBB-GFP-mCherry3E]-[BFP-TDP43 F-S]

    Plasmid

    #133328

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    All F to S in CTD of full-length TDP43

    Available Since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. PHY98-LMNA 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

    Plasmid

    #164046

    Purpose

    CRISPR donor plasmid to insert TriTag (mTagBFP) into the N-terminus of human LMNA gene

    Depositor

    Baohui Chen

    Article

    Xu et al Nucleic Acids Res. 2020 Oct 26. pii: 594

    Insert

    mTagBFG-TriTag
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCVL SFFV HA.NLS.I-AniIY2K227M(reo).T2A.TagBFP

    Plasmid

    #31478

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    SFFV HA NLS Y2 K227M T2A BFP
    

    Use

    Lentiviral

    Tags

    HA and NLS

    Mutation

    F13Y, S111Y, K227M = Y2 nickase "reo" …

    Available Since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCCL-PGK-SPdCas9-BFP-DNMT3B(E697A)

    Plasmid

    #71214

    Purpose

    dCas9 fused to BFP and the human DNMT3B catalytically inactive domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-BFP-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A
    

    Tags

    dSpCas9-BFP-DNMT3B(E697A)

    Expression

    Mammalian

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. RAB11A-BFP HDRT Source (pTR 145)

    Plasmid

    #112014

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with BFP

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    RAB11A-BFP HDRT (RAB11A Human)
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAGGS-mTagBFP2-T2A-sfGFP-iCre-ERT2

    Plasmid

    #128166

    Purpose

    Monomeric TagBFP2 and sfGFP-iCre-ERT2 recombinase (PAPGSTM N-terminus, unskippable linker)

    Depositor

    Mario Capecchi

    Article

    Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

    Insert

    mTagBFP2-T2A-sfGFP-iCre-ERT2
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHBS1527 [IBB-GFP-mCherry3E]-[BFP-TDP43 W-S]

    Plasmid

    #133333

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    All W to S in CTD of full-length TDP43

    Available Since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTRIP-SFFV-mtagBFP-2A STING V155M 354STOP

    Plasmid

    #102595

    Purpose

    Lentivector to express STING V155M delta 354 and mTagBFP

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    V155M delta 354

    Promoter

    SFFV

    Available Since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. CD4-C-BFP HDRT Source (pTR 176)

    Plasmid

    #112020

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with BFP

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    CD4-BFP HDRT (CD4 Human)
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJM450 EF1alpha EBFP2-P2A-BlastR (TUPV1)

    Plasmid

    #139230

    Purpose

    mMoClo TUPV1, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    EBFP2-P2A-Blasticidin S Deaminase
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBW768_pCAG-PV1-FRT-lox2272-BFP-BGHpA

    Plasmid

    #87574

    Purpose

    PV1 for 2-input, 4-output decoder circuit

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    mtagBFP
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Available Since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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