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pRZ366:CMVp-mK2-Ex1-miRS1-mK2-Ex2-PEST-miRS2-BSs-pA

Plasmid

#251331

Purpose

Expression of mKate2 under CMVp promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mKate2

Expression

Mammalian

Promoter

CMVp

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR80:H2A1p-BD6-AS (50bp)2mm(18-19)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA

Plasmid

#251289

Purpose

Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mK2-Ex2

Expression

Mammalian

Promoter

H2A1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR83:H2A1p-BD5-AS(50bp, 50%GC)2mm(18-19)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA

Plasmid

#251291

Purpose

Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mK2-Ex2

Expression

Mammalian

Promoter

H2A1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR85:H2A1p-BD7-AS (30bp)2mm(12-13)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA

Plasmid

#251293

Purpose

Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mK2-Ex2

Expression

Mammalian

Promoter

H2A1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR87:H2A1p-BD8-AS (30bp)2mm(12-13)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA

Plasmid

#251295

Purpose

Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mK2-Ex2

Expression

Mammalian

Promoter

H2A1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR89:H2A1p-BD9-AS (30bp)2mm(12-13)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA

Plasmid

#251297

Purpose

Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mK2-Ex2

Expression

Mammalian

Promoter

H2A1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR91:H2A1p-BD10-AS (30bp)2mm(12-13)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA

Plasmid

#251299

Purpose

Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mK2-Ex2

Expression

Mammalian

Promoter

H2A1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR71:H2A1p-(linker2)-AFP BD-AS(50bp)2mm(18-19)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA

Plasmid

#251283

Purpose

Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mK2-Ex2

Expression

Mammalian

Promoter

H2A1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR72:H2A1p-AFP BD-AS(50bp)2mm(18-19)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA

Plasmid

#251284

Purpose

Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mK2-Ex2

Expression

Mammalian

Promoter

H2A1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 WT 30mer

Plasmid

#231888

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 WT 30mer

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 30mer

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_DBD

Plasmid

#231886

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 DBD

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 DBD

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 WW+

Plasmid

#231885

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 WW+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WW+

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

E109N, S117D, T121W, N126W, T132W

Promoter

T7

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 Swap1

Plasmid

#231879

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 Swap1

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Swap1

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 R3

Plasmid

#231874

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 R3

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 R3

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

D103R, D133R, K143R

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 P+

Plasmid

#231872

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 P+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 P+

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S122P, T131F, A141P, E143D

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 K5

Plasmid

#231866

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 K5

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 K5

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

D103K, D115K, D127K, D133K, E143K

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 E3

Plasmid

#231863

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 E3

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 E3

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

D105E, D118E, D139E

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 W120A

Plasmid

#231862

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 W120A

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

W120A

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Med15

Plasmid

#231887

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 Med15

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Med15

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 R3 W

Plasmid

#231876

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 R3 W

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 R3 W

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

D103R, N126W, D133R, K143R

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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