Addgene

We narrowed to 9,572 results for: HAE

Showing: 1201 - 1220 of 9572 results

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Ppst93-MCRaeo-NG

Plasmid

#192763

Purpose

Expresses NG-tagged MCRaeo in Methanococcus maripaludis

Depositor

William B. Whitman

Article

Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

Insert

Methyl coenzyme M reductase

Use

Synthetic Biology; Heterologous expression in met…

Tags

Flag-Strep2

Expression

Mutation

Promoter

Available since

Dec. 6, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pX458_sgRNA_Ago1_5

Plasmid

#172470

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago1

Depositor

Constance Ciaudo

Article

Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

Insert

sgAgo1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pX458_sgRNA_Ago1_6

Plasmid

#172471

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago1.

Depositor

Constance Ciaudo

Article

Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

Insert

sgAgo1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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MPC021: CamKII CMV_sNovArch_citrine (soma targeting)

Plasmid

#153194

Purpose

encoded soma targeted NovArch

Depositor

Adam Cohen

Article

Chien et al Sci Adv. 2021 May 5;7(19). pii: 7/19/eab

Insert

sNovArch

Use

Tags

citrine

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pL2-1 ConjEpi PtEF2bsd Pt49202HMGRVenusThrFLAG

Plasmid

#139963

Purpose

Over-expression of native 3-hydroxy-3-methyl glutaryl CoA reductase

Depositor

Raffaela Abbriano

Article

Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

Insert

ConjEpi PtEF2_bsd_PttEF2 Pt49202_HMGRVenusThrFLAG_Ptt49202

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL-SsoPCNA3

Plasmid

#107069

Purpose

Expresses S. solfataricus PCNA3 fused to MBP in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA3

Use

Tags

6xHis-thrombin cleavage site and MBP

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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jam1a-pCRII

Plasmid

#68803

Purpose

For in situ antisense probe, NotI w/ Sp6

Depositor

David Traver

Article

Kobayashi et al Nature. 2014 Aug 21;512(7514):319-23. do

Insert

jam1a (f11r.1 Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only
pET11+PCNA3-CYP119

Plasmid

#66136

Purpose

Expresses the wild type of CYP119 fused to Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

Insert

CYP119 (cyp119 )

Use

Tags

Sulfolobus solfataricus PCNA3

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pET11+PCNA3-CYP119(T214V)

Plasmid

#66137

Purpose

Expresses the T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

Insert

CYP119 (cyp119 )

Use

Tags

Sulfolobus solfataricus PCNA3

Expression

Bacterial

Mutation

T214V

Promoter

T7 promoter

Available since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET11+PCNA3-CYP119(D77R/T214V)

Plasmid

#66138

Purpose

Expresses the D77R/T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

Insert

CYP119 (cyp119 )

Use

Tags

Sulfolobus solfataricus PCNA3

Expression

Bacterial

Mutation

D77R, T214V

Promoter

T7 promoter

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-mIFITM1-C103A

Plasmid

#58419

Purpose

Expresses murine IFITM1-C103A with an N-terminal HA tag in mammalian cells.

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Cysteine 103 to Alanine

Promoter

CMV IE

Available since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM1-C83A

Plasmid

#58418

Purpose

Expresses murine IFITM1-C83A with an N-terminal HA tag in mammalian cells.

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Cysteine 83 to Alanine

Promoter

CMV IE

Available since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM1-C50A

Plasmid

#58417

Purpose

Expresses murine IFITM1-C50A with an N-terminal HA tag in mammalian cells.

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Cysteine 50 to Alanine

Promoter

CMV IE

Available since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM1-C49A

Plasmid

#58416

Purpose

Expresses murine IFITM1-C49A with an N-terminal HA tag in mammalian cells

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Cysteine 49 to Alanine

Promoter

CMV IE

Available since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
AF0837

Plasmid

#19091

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Cell division protein pelota (DOM34 Archaeoglobus fulgidus)

Use

Tags

C tag

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
pRSF-G1(7FTrp)RS

Plasmid

#177310

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al ACS Sens. 2022 Jan 10. doi: 10.1021/acss

Insert

tRNA synthetase

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-NLS-3xFlag

Plasmid

#132402

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1059A

Promoter

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPspCas13b-EGFP-NLS-3xFlag

Plasmid

#132397

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1058A

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-3xFlag

Plasmid

#132403

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1059A

Promoter

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPguCas13b-EGFP-NLS-3xFlag

Plasmid

#132400

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPguCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H151A; H1121A

Promoter

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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