Addgene

We narrowed to 5,865 results for: pcDNA 3.1

Showing: 1831 - 1860 of 5865 results

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pcDNA3.1_Linear Tau 0N4R 3X Flag tag

Plasmid

#194171

Purpose

Insert contains linear tau 0N4R with a 3X Flag tag. Expresses linear protein tau 0N4R with a 3X Flag tag for Immunoprecipitation.

Depositor

Stefan Stamm

Article

Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

Insert

Microtubule-Associated Protein Tau 0N4R

Use

Tags

3X Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A HIS-TEV-DPP9S

Plasmid

#166791

Purpose

Mammalian cell expression vector for His-tagged DPP9S

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Use

Tags

HIS-TEV

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcdna 3.1 DSG-3 Tension Sensor

Plasmid

#182717

Purpose

expresses DSG-3 TSmod

Depositor

Daniel Conway

Article

Jin et al bioRxiv [Preprint]. 2024 May 6:2024.05.0

Insert

desmoglein-3 (Dsg3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

TSmod inserted between G726 and G727

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) mouse C/EBP gamma

Plasmid

#12560

Depositor

Peter Johnson

Article

Parkin et al J Biol Chem. 2002 Jun 28;277(26):23563-7

Insert

CCAAT enhancer binding protein gamma (Cebpg Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-dCas9-MQ1(S317A)-EGFP

Plasmid

#89636

Purpose

Targeted CpG methylation

Depositor

Margaret Goodell

Article

Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

Insert

site-specific DNA-methyltransferase SssI

Use

CRISPR

Tags

3*FLAG and 6*His

Expression

Mammalian

Mutation

S317A, AGC-->GCC

Promoter

CMV

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Kozak-SP-SNAPf-lk-EK-ADGRL3-CTF

Plasmid

#201217

Purpose

pCDNA3.1 with Kozak (GCCACC), ADGRL3 signal peptide, SNAP-tag, GS linker, and Flag-tag (EK site) ahead of ADGRL3-CTF

Depositor

Jonathan Javitch

Article

Perry-Hauser et al J Biol Chem. 2022 Dec;298(12):102594. do

Insert

ADGRL3 (Adgrl3 Mouse)

Use

Tags

Flag and SNAP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLAG-SIV-RCM-vpx

Plasmid

#115844

Purpose

Encodes codon optimized FLAG tagged SIVRCM-Vpx

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVRCM-Vpx

Use

Tags

3x-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Myc-His(-)-HSulf-1 deltaCC

Plasmid

#13005

Depositor

Steven Rosen

Article

Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

Insert

human sulfatase 1 delta CC (SULF1 Human)

Use

Tags

Expression

Mammalian

Mutation

changed Cysteine 87 to Alanine

Promoter

Available since

Oct. 30, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-CD81-(TEV)-FKBP-Myc

Plasmid

#177908

Purpose

human CD81-TEV cleavage site-FKBP-Myc tag

Depositor

Masaharu Somiya

Article

Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

Insert

human CD81 (CD81 Human, Synthetic)

Use

Tags

FKBP, Myc tag, and TEV protease cleavage site

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNATM3.1/V5-His TOPO-PKCi-K274W

Plasmid

#55690

Purpose

expresses dominant negative human PKCi tagged with V5

Depositor

Pedro Salas

Article

Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

Insert

Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)

Use

Tags

V5/His

Expression

Mammalian

Mutation

A120E and K274W

Promoter

CMV

Available since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 LIC 6A HIS-TEV-DPP8

Plasmid

#166793

Purpose

Mammalian cell expression vector for Flag-tagged DPP8

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP8 (DPP8 Human)

Use

Tags

HIS-TEV

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 S D614G-trunc

Plasmid

#158077

Purpose

Expresses SARS-CoV-2 spike protein with a D614G truncated mutation

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 spike (S )

Use

Tags

Expression

Mammalian

Mutation

D614G

Promoter

Available since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A FLAG-TEV-DPP8

Plasmid

#166795

Purpose

Mammalian cell expression vector for FLAG-tagged DPP8

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP8 (DPP8 Human)

Use

Tags

FLAG-TEV

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-2xSTREP-CCND1(T286A)

Plasmid

#172647

Purpose

Expresses 2xFLAG-2xSTREP-tagged cyclin D1(T286A) in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Thr286Ala

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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NC3 pCDNA3.1 FLAG WTX (1-804)

Plasmid

#36958

Depositor

Randall Moon

Article

Major et al Science. 2007 May 18;316(5827):1043-6.

Insert

WTX (AMER1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)/AUG-nLuc-3XFLAG-PEST

Plasmid

#127312

Purpose

Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from AUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG and PEST

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNATM3.1/V5-His TOPO-PKCi-A120E

Plasmid

#55687

Purpose

expresses constitutively active human PKCi tagged with V5

Depositor

Pedro Salas

Article

Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

Insert

Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)

Use

Tags

V5/His

Expression

Mammalian

Mutation

A120E

Promoter

CMV

Available since

July 28, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 LIC 6A CARD8-FLAG S297A

Plasmid

#169949

Purpose

Mammalian expression of CARD8-FLAG that is autoprocessing-deficient (S297A)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S297A

Promoter

CMV

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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(137) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4)

Plasmid

#160740

Purpose

Expresses a truncated OGT (4.5 TPRs) fused to the EPEA nanobody

Depositor

Christina Woo

Article

Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

Insert

HA-nEPEA-(EAAAK)4-OGT(4)

Use

Tags

HA-Tag

Expression

Mammalian

Mutation

Promoter

T7/CMV

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Myr-DnaJB1-PKAcat-mCherry

Plasmid

#181853

Purpose

mCherry-tagged DnaJB1-PKA catalytic subunit fusion with N-terminal myristoylation site added; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

Myr-DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Laccase2 MCS-ciRS7 Exon

Plasmid

#69900

Purpose

Expresses the human ciRS7/CDR1as circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ciRS7 (stw, CDR1-AS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-dCas9-MQ1(C141S)-EGFP

Plasmid

#89635

Purpose

Targeted CpG methylation

Depositor

Margaret Goodell

Article

Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

Insert

site-specific DNA-methyltransferase SssI

Use

CRISPR

Tags

3*FLAG, 6*His, and T2A-EGFP

Expression

Mammalian

Mutation

C141S, TGC-->TCC

Promoter

CMV

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-tvD-Cit-tevs-DHFR

Plasmid

#116054

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

tvD-Cit-tevs-DHFR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-) Full length Her2(ERBB2)-Fc

Plasmid

#194330

Purpose

Mammalian expression of full length Human epidermal growth factor (Her)-2-Fc fusion protein

Depositor

Alexander McLellan

Insert

Her2-Fc (ERBB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag HBx (G124L, I127A)

Plasmid

#42598

Depositor

Ding Xue

Article

Geng et al Proc Natl Acad Sci U S A. 2012 Nov 6;109

Insert

Hepatitis B virus protein X (G124L, I127A)

Use

Tags

Flag

Expression

Mammalian

Mutation

G124L I127A

Promoter

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+) ZKSCAN1 MCS-HIPK3 Exon

Plasmid

#69905

Purpose

Expresses the human HIPK3 exon 2 circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

HIPK3 (HIPK3 Human, Fly)

Use

Tags

Expression

Mammalian

Mutation

Expresses aa1-194 of HIPK3

Promoter

CMV

Available since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CIP2A(1-905) R522D V5 His

Plasmid

#119289

Purpose

CIP2A R522D V5 overexpression, CIP2A dimer defective mutant

Depositor

Jukka Westermarck

Article

Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

Insert

KIAA1524 (CIP2A Human)

Use

Tags

V5 His

Expression

Mammalian

Mutation

R522D

Promoter

T7

Available since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 GIL-11 myc TEV TwinStrep

Plasmid

#199627

Purpose

Eucaryotic expression plasmid for chimeric cytokine of IL-11 and LIF (GIL-11) with a TwinStrep-tag

Depositor

Jurgen Scheller

Article

Rafii et al Commun Biol. 2023 Apr 15;6(1):418. doi:

Insert

GIL-11

Use

Tags

TwinStrep-tag

Expression

Mammalian

Mutation

Promoter

T7

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-CIBN-mCherry-VAMP2

Plasmid

#178573

Purpose

Mammalian expression plasmid of CIBN-VAMP2 of Opto-vTrap

Depositor

Won Do Heo

Article

Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

Insert

CIBN-mCherry-VAMP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 S D614-trunc

Plasmid

#158076

Purpose

Expresses SARS-CoV-2 spike protein with a truncation

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 spike (S )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge