We narrowed to 13,035 results for: Mpl

1. pAG415Gal_Gcn4 WW+HA

   Plasmid

   #232991

   Purpose

   Galactose iduced expression of Gcn4 WW+HA in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 WW+
   

   Use

   Tags

   3xHA and TEV cleavage site

   Expression

   Yeast

   Mutation

   E109N, S117D, T121W, N126W, T132W

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAG415Gal_Gcn4 WW+GFP

   Plasmid

   #233003

   Purpose

   Galactose iduced expression of Gcn4 WW+GFP in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 WW+
   

   Use

   Tags

   HA-GFP-HA and TEV cleavage site

   Expression

   Yeast

   Mutation

   E109N, S117D, T121W, N126W, T132W

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAG415Gal_Gcn4 W120AGFP

   Plasmid

   #233004

   Purpose

   Galactose iduced expression of Gcn4 120AGFP in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 W120A
   

   Use

   Tags

   HA-GFP-HA and TEV cleavage site

   Expression

   Yeast

   Mutation

   W120A

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAG415Gal_Gcn4 Aro2+GFP

   Plasmid

   #233001

   Purpose

   Galactose iduced expression of Gcn4 Aro2+GFP in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 Aro2+
   

   Use

   Tags

   HA-GFP-HA and TEV cleavage site

   Expression

   Yeast

   Mutation

   N116F, S117E, E119N, P129Q, T132Y

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAG415Gal_Gcn4 S/ArotoL

   Plasmid

   #232979

   Purpose

   Galactose iduced expression of Gcn4 S/ArotoL in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 S/ArotoL
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   S101L, S104L, F108L, Y110L, S117L, S136L, S144L

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAG415Gal_Gcn4 P+

   Plasmid

   #232971

   Purpose

   Galactose iduced expression of Gcn4 Proline in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 Proline
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   S122P, T131F, A141P, E143D

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAG415Gal_Gcn4 9acidA

   Plasmid

   #232967

   Purpose

   Galactose iduced expression of Gcn4 9acidA in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 9acidA
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   E109A, E111A, E114A, D115A, E119A, D125A, D127A, …

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAG415Gal_Gcn4 E2K2

   Plasmid

   #232968

   Purpose

   Galactose iduced expression of Gcn4 EK+ in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 EK+
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   S104K, S117E, S136E, S144K

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ppAG415Gal_Gcn4 L113A+

   Plasmid

   #232972

   Purpose

   Galactose iduced expression of Gcn4 L113A in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 L113A
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   L113A, E114Y, N126H, D127E, S136A

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAG415Gal_Gcn4 Aro3+GFP

    Plasmid

    #233002

    Purpose

    Galactose iduced expression of Gcn4 Aro3+GFP in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 Aro3+
    

    Use

    Tags

    HA-GFP-HA and TEV cleavage site

    Expression

    Yeast

    Mutation

    D103Y, N112T, L113Y, S117F, K140R

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAG415Gal_Gcn4 ILVtoED

    Plasmid

    #232957

    Purpose

    Galactose iduced expression of Gcn4 ILVtoED in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 ILVtoED
    

    Use

    Tags

    TEV cleavage site

    Expression

    Yeast

    Mutation

    L123D, I128E, V130E, V135E, L137D, I142D

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAG415Gal_Gcn4 StoL

    Plasmid

    #232981

    Purpose

    Galactose iduced expression of Gcn4 StoL in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 StoL
    

    Use

    Tags

    TEV cleavage site

    Expression

    Yeast

    Mutation

    S104L, S117L, S136L, S144L

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAG415Gal_Gcn4 R3W

    Plasmid

    #232975

    Purpose

    Galactose iduced expression of Gcn4 R++ W+ in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 R++ W+
    

    Use

    Tags

    3xHA and TEV cleavage site

    Expression

    Yeast

    Mutation

    D103R, N126W, D133R, K143R

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAG415Gal_Gcn4 E3HA

    Plasmid

    #232996

    Purpose

    Galactose iduced expression of Gcn4 E+HA in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 E+
    

    Use

    Tags

    3xHA and TEV cleavage site

    Expression

    Yeast

    Mutation

    D105E, D118E, D139E

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAG415Gal_Gcn4 F108A+

    Plasmid

    #232974

    Purpose

    Galactose iduced expression of Gcn4 F108A in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 F108A
    

    Use

    Tags

    TEV cleavage site

    Expression

    Yeast

    Mutation

    F108A, T121L, A141P

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAG415Gal_Gcn4 ILVtoAS

    Plasmid

    #232959

    Purpose

    Galactose iduced expression of Gcn4 ILVtoAS in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 ILVtoAS
    

    Use

    Tags

    TEV cleavage site

    Expression

    Yeast

    Mutation

    L123S, I128S, V130A, V135A, L137S, I142S

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDest17_Gcn4 S/ArotoL

    Plasmid

    #231880

    Purpose

    Bacterial expression of N-terminally 6His tagged Gcn4 SArotoL

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 S/ArotoL
    

    Use

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Mutation

    S101L, S104L, F108L, Y110L, S117L, S136L, S144L

    Promoter

    T7

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDest17_Gcn4 solvvol W

    Plasmid

    #231878

    Purpose

    Bacterial expression of N-terminally 6His tagged Gcn4 solvvol W

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 solvvol W
    

    Use

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Mutation

    S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

    Promoter

    T7

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDest17_Gcn4 solvvol

    Plasmid

    #231877

    Purpose

    Bacterial expression of N-terminally 6His tagged Gcn4 solvvol

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 solvvol
    

    Use

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Mutation

    S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

    Promoter

    T7

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDest17_Gcn4 9acidA

    Plasmid

    #231867

    Purpose

    Bacterial expression of N-terminally 6His tagged Gcn4 9acidA

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 9acidA
    

    Use

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Mutation

    E109A, E111A, E114A, D115A, E119A, D125A, D127A, …

    Promoter

    T7

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pXD80EN5-Elen0515-eCadh-noAccs(ElA2)

    Plasmid

    #225313

    Purpose

    Expresses Eggerthella lenta A2 eCadh without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213

    Depositor

    Emily Balskus

    Article

    Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

    Insert

    eCadh (Elen_0515 )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pXD80ENSF7.1-Dadh-ΔElen0469(ElA2)

    Plasmid

    #225315

    Purpose

    Expresses Eggerthella lenta A2 Dadh (ΔElen_0469) in Gordonibacter urolithinfaciens DSM 27213

    Depositor

    Emily Balskus

    Article

    Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

    Insert

    Dadh (ΔElen_0469)
    

    Use

    Tags

    His8

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pXD80ENSF7.4-Dadh-ΔElen0474(ElA2)

    Plasmid

    #225319

    Purpose

    Expresses Eggerthella lenta A2 Dadh (ΔElen_0474) in Gordonibacter urolithinfaciens DSM 27213

    Depositor

    Emily Balskus

    Article

    Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

    Insert

    Dadh (ΔElen_0474)
    

    Use

    Tags

    His8

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pXD80ENSF7.7-Dadh-ΔElen0477(ElA2)

    Plasmid

    #225322

    Purpose

    Expresses Eggerthella lenta A2 Dadh (ΔElen_0477) in Gordonibacter urolithinfaciens DSM 27213

    Depositor

    Emily Balskus

    Article

    Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

    Insert

    Dadh (ΔElen_0477)
    

    Use

    Tags

    His8

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pXD80ENSF7.8-Dadh-ΔElen0478(ElA2)

    Plasmid

    #225323

    Purpose

    Expresses Eggerthella lenta A2 Dadh (ΔElen_0478) in Gordonibacter urolithinfaciens DSM 27213

    Depositor

    Emily Balskus

    Article

    Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

    Insert

    Dadh (ΔElen_0478)
    

    Use

    Tags

    His8

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pXD80ENSF7.9-Dadh-ΔElen0481(ElA2)

    Plasmid

    #225324

    Purpose

    Expresses Eggerthella lenta A2 Dadh (ΔElen_0481) in Gordonibacter urolithinfaciens DSM 27213

    Depositor

    Emily Balskus

    Article

    Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

    Insert

    Dadh (ΔElen_0481)
    

    Use

    Tags

    His8

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pXD80ENSF7.10-Dadh-ΔElen0482(ElA2)

    Plasmid

    #225325

    Purpose

    Expresses Eggerthella lenta A2 Dadh (ΔElen_0482) in Gordonibacter urolithinfaciens DSM 27213

    Depositor

    Emily Balskus

    Article

    Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

    Insert

    Dadh (ΔElen_0482)
    

    Use

    Tags

    His8

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pXD70DA8-DadhA(cHis8)BC-DadRS-noAccs(ElA2)

    Plasmid

    #225326

    Purpose

    Expresses Eggerthella lenta A2 Dadh in Eggerthella lenta DSM 2243

    Depositor

    Emily Balskus

    Article

    Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

    Insert

    Dadh
    

    Use

    Tags

    His8

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pXD80ENSF6-Elen0497-ElVadh-Accs(ElDSM2243)

    Plasmid

    #225301

    Purpose

    Expresses Eggerthella lenta DSM 2243 El Vadh in Gordonibacter urolithinfaciens DSM 27213

    Depositor

    Emily Balskus

    Article

    Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

    Insert

    El Vadh
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pXD80ENSF7-DadhA(cHis8)BC-DadRS-Accs(ElA2)

    Plasmid

    #225302

    Purpose

    Expresses Eggerthella lenta A2 Dadh in Gordonibacter urolithinfaciens DSM 27213

    Depositor

    Emily Balskus

    Article

    Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

    Insert

    Dadh
    

    Use

    Tags

    His8

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pXD80ENSF8-HcdhA(cHis8)BC-HcdR-Accs(ElA2)

    Plasmid

    #225303

    Purpose

    Expresses Eggerthella lenta A2 Hcdh in Gordonibacter urolithinfaciens DSM 27213

    Depositor

    Emily Balskus

    Article

    Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

    Insert

    Hcdh
    

    Use

    Tags

    His8

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pXD80ENSF18-C1878_02750_GsVadh(Gs28C)

    Plasmid

    #225304

    Purpose

    Expresses Gordonibacter sp. 28C Gs Vadh in Gordonibacter urolithinfaciens DSM 27213

    Depositor

    Emily Balskus

    Article

    Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

    Insert

    Gs Vadh
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pXD80EN3-Elen0507-Cadh-noAccs(ElA2)

    Plasmid

    #225311

    Purpose

    Expresses Eggerthella lenta A2 Cadh without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213

    Depositor

    Emily Balskus

    Article

    Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

    Insert

    Cadh
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pXD80EN4-Elen0511-(–)-Eldh1-noAccs(ElA2)

    Plasmid

    #225312

    Purpose

    Expresses Eggerthella lenta A2 (–)-Eldh1 without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213

    Depositor

    Emily Balskus

    Article

    Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

    Insert

    (–)-Eldh1 (Elen_0511 )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pXD80ENSF3-Elen0507-Cadh-Accs(ElA2)

    Plasmid

    #225298

    Purpose

    Expresses Eggerthella lenta A2 Cadh in Gordonibacter urolithinfaciens DSM 27213

    Depositor

    Emily Balskus

    Article

    Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

    Insert

    Cadh
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pXD70EN25-Pct5-(–)-Eddh(Gp3C)

    Plasmid

    #233877

    Purpose

    Expresses Gordonibacter pamelaeae 3C (–)-Eddh in Eggerthella lenta DSM 2243 with a cumate inducible promoter

    Depositor

    Emily Balskus

    Article

    Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

    Insert

    (–)-Eddh
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMR-A-3-Sasg

    Plasmid

    #214349

    Purpose

    Expresses T7-RNAP, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    T7-RNAP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDest17_Gcn4 WTSLFD

    Plasmid

    #231881

    Purpose

    Bacterial expression of N-terminally 6His tagged Gcn4 WTSLFD

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 WTSLFD
    

    Use

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Mutation

    F108W, E109T, Y110S, E111L, N112F, L113D

    Promoter

    T7

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDest17_Gcn4 ILVtoED W

    Plasmid

    #231871

    Purpose

    Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED W

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 ILVtoED W
    

    Use

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Mutation

    N126W, I128E, V130E, V135E, L137D, I142D

    Promoter

    T7

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDest17_Gcn4 ILVtoAS

    Plasmid

    #231859

    Purpose

    Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoAS

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 ILVtoAS
    

    Use

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Mutation

    L123S, I128S, V130A, V135A, L137S, I142S

    Promoter

    T7

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pDest17_Gcn4 ILVtoED

    Plasmid

    #231857

    Purpose

    Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 ILVtoED
    

    Use

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Mutation

    L123D, I128E, V130E, V135E, L137D, I142D

    Promoter

    T7

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pTYB1-T4gp47

    Plasmid

    #228212

    Purpose

    Expression in BL21(DE3) of T4 SbcD-like subunit of palindrome specific endonuclease

    Depositor

    Stephen Benkovic

    Article

    Herdendorf et al J Biol Chem. 2011 Jan 28;286(4):2382-92.

    Insert

    47 (47 Escherichia phage T4)
    

    Use

    Tags

    Sce VMA intein-chitin binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pGGA016

    Plasmid

    #228231

    Purpose

    Level 0 for 5xOp (GreenGate A module)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    5xOp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pFA6a-BirA-HA3-HIS3MX6

    Plasmid

    #227975

    Purpose

    To insert HA-tagged biotin ligase, BirA, at the C-terminus of genes; yeast BioID, HIS3MX6 marker

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    BirA
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pRS416-CUP1p-TID-FRB-HA3

    Plasmid

    #228008

    Purpose

    Expresses TurboID-FRB fusion from CUP1 promoter; yeast BioID

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID-FRB
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pRS416-CUP1p-TID-FRB-NLS-HA3

    Plasmid

    #228009

    Purpose

    Expresses TurboID-FRB-NLS fusion from CUP1 promoter; yeast BioID

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID-FRB
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pRS416-CUP1p-TID-FKBP-HA3

    Plasmid

    #228010

    Purpose

    Expresses TurboID-FKBP fusion from CUP1 promoter; yeast BioID

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID-FKBP
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pRS416-CUP1p- TID-FKBP-NLS-HA3

    Plasmid

    #228011

    Purpose

    Expresses TurboID-FKBP-NLS fusion from CUP1 promoter; yeast BioID

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID-FKBP
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pRS416-CHA1p-TID-FRB-HA3

    Plasmid

    #228012

    Purpose

    Expresses TurboID-FRB fusion from CHA1 promoter; yeast BioID

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID-FRB
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    CHA1

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pRS416-CHA1p-TID-FRB-NLS-HA3

    Plasmid

    #228013

    Purpose

    Expresses TurboID-FRB-NLS fusion from CHA1 promoter; yeast BioID

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID-FRB
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    CHA1

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge