We narrowed to 7,571 results for: Lif

1. RCP83

   Plasmid

   #163466

   Purpose

   Backbone plasmid for insertion of the Saccharomyces cerevisiae promoter library and yeGFP

   Depositor

   Frank Albert

   Article

   Renganaath et al Elife. 2020 Nov 12;9. pii: 62669. doi: 1

   Inserts

   Promoter fragment (Sharon2012_1922)
   His3 minimal promoter
   yeGFP
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. nontarget S. pyogenes scaffold CARGO array

   Plasmid

   #191319

   Purpose

   Contains an array of nontargeting guide RNAs

   Depositor

   Joanna Wysocka

   Article

   Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

   Insert

   Array of guide RNAs
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-TRE-DIO-TVA-oG-mCherry

   Plasmid

   #166602

   Purpose

   Encodes Cre-dependent TVA-oG-mCherry under control of the TRE

   Depositor

   Martin Myers

   Article

   Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

   Insert

   TVA-oG-mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   tetracycline response element

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. 35S-GWY-GAL4BD/pAM

   Plasmid

   #201231

   Purpose

   Used to produce and express in planta N-terminal fusion proteins with the GAL4 BD

   Depositor

   Michael Ayliffe

   Article

   Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

   Insert

   35S-GWY-GAL4BD
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLX304-Flag-APEX2-NES

   Plasmid

   #92158

   Purpose

   Cytosolic APEX2 construct

   Depositor

   Alice Ting

   Article

   Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

   Insert

   Flag-APEX2-NES
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ATM-Halo HRD

   Plasmid

   #207082

   Purpose

   Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous ATM locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   HaloTag followed by a PolyA signal and PuroR cassette flanked by human ATM locus sequences
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   None

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. P362-FBP_12

   Plasmid

   #139703

   Purpose

   T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway

   Depositor

   Daniel Voytas

   Article

   Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

   Insert

   pSlH4:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtuMas:NhH3H:tAtuMas, p35S:NnHisps:t35S, p35S:NnLuz:AtuOcs
   

   Use

   Luciferase

   Tags

   Expression

   Plant

   Mutation

   Promoter

   various

   Available since

   May 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV.hSyn.GLPLight1

   Plasmid

   #187466

   Purpose

   Expresses GLPLight1 in neurons

   Depositor

   Tommaso Patriarchi

   Article

   Duffet et al Elife. 2023 Jun 2;12:RP86628. doi: 10.75

   Insert

   GLPLight1
   

   Use

   AAV

   Tags

   Flag tag

   Expression

   Mutation

   Promoter

   human Synapsin-1

   Available since

   Feb. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 15x-QUAS-dTomato-T2A-GCaMP6s

   Plasmid

   #130666

   Purpose

   QUAS reporter line that expresses cytosolic dTomato and the GCaMP6s calcium indicator, separated by T2A

   Depositor

   Leslie Vosshall

   Article

   Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

   Insert

   15xQUAS-dTomato-T2A-GCaMP6s
   

   Use

   Ae. aegypti expression

   Tags

   Expression

   Insect

   Mutation

   Promoter

   15xQUAS-TATA

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HLTV-hTRIM21

    Plasmid

    #104973

    Purpose

    Purification of His-Lipoyl-hTRIM21 protein from bacteria

    Depositor

    Leo James

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    Tripartite Motif containing 21 (TRIM21 Human)
    

    Use

    Tags

    His-Lipoyl

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAGGS-Bxb1o_nlsFlag

    Plasmid

    #119901

    Purpose

    Expresses Bxb1 integrase in mammalian cells

    Depositor

    Orion Weiner

    Article

    Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

    Insert

    Mammalian codon optimized Bxb1 integrase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-SEP-GluA2 TKIT

    Plasmid

    #169442

    Purpose

    Expression of 2 guides + donor DNA

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    Super ecliptic pHluorin (SEP)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Halo-MDC1

    Plasmid

    #207108

    Purpose

    pHTN HaloTag CMV-neo (Promega; #G7721) based vector to express HaloTag-MDC1 in human cells.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    HaloTag-MDC1
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. flex-ChrimsonR-EYFP-Kv

    Plasmid

    #135319

    Purpose

    Cre-dependent expression of soma-targeted ChrimsonR

    Depositor

    Hongkui Zeng

    Article

    Hage et al Elife. 2022 Jan 21;11:e71103. doi: 10.75

    Insert

    ChrimsonR
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    synapsin

    Available since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 4xmts-G-Ca-FLITS

    Plasmid

    #191462

    Purpose

    Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    G-Ca-FLITS
    

    Use

    Tags

    4xmts

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. acta1-mRuby2-dGBP

    Plasmid

    #163855

    Purpose

    Zebrafish muscle specific expression of mRuby2-dVHH[GFP]

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    dVHH[GFP]
    

    Use

    In vitro transcription of rna

    Tags

    Ruby2

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. acta1-mRuby2-VHH[GFP]

    Plasmid

    #163856

    Purpose

    Zebrafish muscle specific expression of mRuby2-VHH[GFP]. Parton lab clone FRX

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    VHH[GFP]
    

    Use

    In vitro transcription of rna

    Tags

    Ruby2

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pT3TS-CD44b-TurboID

    Plasmid

    #163850

    Purpose

    In vitro transcription of CD44b-TurboID. Parton lab clone JTJ

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    CD44b (cd44b Zebrafish)
    

    Use

    In vitro transcription of rna

    Tags

    TurboID

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCEP-Puro-TetOn-L1RP-ORF1_R261A_Halo-GFPai

    Plasmid

    #202569

    Purpose

    Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the R261A mutation and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    LINE-1
    

    Use

    Tags

    GFP-AI retrotransposition reporter and HaloTag7 o…

    Expression

    Mammalian

    Mutation

    Changed ORF1p Arginine 261 to Alanine

    Promoter

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMJ915

    Plasmid

    #69090

    Purpose

    Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLS

    Depositor

    Jennifer Doudna

    Article

    Lin et al Elife. 2014 Dec 15;3:e04766. doi: 10.755

    Insert

    cas9
    

    Use

    Tags

    MBP, hexa His, and two SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge