Addgene

We narrowed to 7,339 results for: Lif

Showing: 1221 - 1240 of 7339 results

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flex-ChrimsonR-EYFP-Kv

Plasmid

#135319

Purpose

Cre-dependent expression of soma-targeted ChrimsonR

Depositor

Hongkui Zeng

Article

Hage et al Elife. 2022 Jan 21;11:e71103. doi: 10.75

Insert

ChrimsonR

Use

AAV

Tags

EYFP

Expression

Mutation

Promoter

synapsin

Available since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-FIP3

Plasmid

#74753

Purpose

Expressed sfGFP-tagged FIP3 in E. coli for protein production

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

Rab11 family interacting protein 3 (RAB11FIP3 Human)

Use

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 13, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV7 mitoDendra2

Plasmid

#157757

Purpose

Expression of the fluorescent protein Dendra2 targeted to mitochondria

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

Dendra2

Use

Tags

Expression

Mammalian

Mutation

MTS coxVIII +Dendra2

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pUE1-TSP-BFLF1

Plasmid

#162657

Purpose

Expresses BFLF1 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

BFLF1

Use

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pPW3756 : CMVd3-ERmembrane-HaloTag-KKMP

Plasmid

#185678

Purpose

Low-level transient expression of a single HaloTag protein with a C-terminal KKMP ER retention signal, targeted to the ER membrane.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERmembrane-HaloTag-KKMP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-RT003-GPM

Plasmid

#163383

Purpose

To express sGFP module in the G-baToN system

Depositor

Monte Winslow

Article

Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

Insert

GFP-PDGFRtm-2A-mCherry (GPM)

Use

Lentiviral

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pH7m34GW-35S:SNACS S785A

Plasmid

#203577

Purpose

Plant SnRK2S30A kinase activity FRET sensor in plant expression vector

Depositor

Julian Schroeder

Article

Zhang et al eLife. 2020 May 28;9:e56351. doi: 10.755

Insert

SNACS S785A

Use

Tags

StrepII

Expression

Plant

Mutation

S785A

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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Halo-MDC1

Plasmid

#207108

Purpose

pHTN HaloTag CMV-neo (Promega; #G7721) based vector to express HaloTag-MDC1 in human cells.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

HaloTag-MDC1

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-SAC1

Plasmid

#108127

Purpose

Recruitable SAC1

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L (SACM1L Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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OSCA1.2

Plasmid

#136593

Purpose

Expresses the mechanosensitive channel OSCA1.2 in mammalian cells

Depositor

Ardem Patapoutian

Article

Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

Insert

OSCA1.2 (AT4G22120 Mustard Weed)

Use

Tags

pIRES2-mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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RNF168-Halo HRD

Plasmid

#207080

Purpose

Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF168 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

HaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences

Use

Tags

Expression

Mammalian

Mutation

Promoter

None

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-puro-kozak-scFv-GCN4-HAtag-msfGFP(V206K)-GBI

Plasmid

#205162

Purpose

express scFv-GCN4-HAtag-msfGFP(V206K)-GBI

Depositor

Weirui Ma

Article

Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

Insert

scFv-GCN4-HAtag-msfGFP(V206K)-GBI

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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LTR5HS S. aureus scaffold CARGO array

Plasmid

#191317

Purpose

Contains an array of guide RNAs targeting LTR5Hs elements

Depositor

Joanna Wysocka

Article

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Insert

Array of guide RNAs

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g4

Plasmid

#80787

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 4/14/24 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g1

Plasmid

#80784

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 1/11/21 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pDAS12125_PEAR-GFP

Plasmid

#177178

Purpose

PEAR-GFP target plasmid with split EGFP and disrupted 5' splice site

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Tags

Expression

Mammalian

Mutation

disrupted 5' splice site

Promoter

CMV

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g2

Plasmid

#80785

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 2/12/22 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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LTR5HS S. pyogenes scaffold CARGO array

Plasmid

#191316

Purpose

Contains an array of guide RNAs targeting LTR5Hs elements

Depositor

Joanna Wysocka

Article

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Insert

Array of guide RNAs

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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SB_convex

Plasmid

#110104

Purpose

pBXPHM3 with scaffold Sybody of the convex library

Depositor

Markus Seeger

Article

Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

Insert

scaffold Sybody of the convex library

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

PBAD

Available since

May 24, 2018

Availability

Academic Institutions and Nonprofits only

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P336-FBP_11

Plasmid

#139702

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing Luz

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNPGA:tAtug7, pCmYLCV:NnH3H:AtuOcs, pCmYLCV:NnHisps:tHsp

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

various

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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