We narrowed to 16,982 results for: bon

1. TVBB C-term-mTurquoise2

   Plasmid

   #169223

   Purpose

   Targeting vector backbone to support a knock-in of Linker-mTurquoise2 at the C-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked mTurquoise2
   

   Use

   Targeting vector backbone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn1-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA

   Plasmid

   #68720

   Purpose

   Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.

   Depositor

   Mark Huebener, Tobias Bonhoeffer, Tobias Rose

   Article

   Rose et al Science. 2016 Jun 10;352(6291):1319-22.

   Has Service

   AAV1

   Insert

   mRuby2-P2A-GCaMP6s
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Cre-dependent expression from inverted open readi…

   Promoter

   hSyn1-FLEX

   Available since

   June 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCh-KIF1A*-strep

   Plasmid

   #120165

   Purpose

   Expresses a chimera of mCherry (fluorescent tag), a KIF1A-based anterograde motor and streptavidin

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   kinesin family member 1A (Kif1a Mouse)
   

   Use

   Tags

   Streptavidin and mCherry

   Expression

   Mammalian

   Mutation

   Truncated KIF1A: 1-405 aa

   Promoter

   CMV

   Available since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Strep-KIFC1*-HA

   Plasmid

   #120171

   Purpose

   Expresses a chimera of streptavidin, a KIFC1-based retrograde motor and HA tag

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   kinesin family member C1 (KIFC1 Human)
   

   Use

   Tags

   HA and Streptavidin

   Expression

   Mammalian

   Mutation

   Truncated KIFC1: 125-673 aa

   Promoter

   CMV

   Available since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Str-STIM1-NN_puromycin

   Plasmid

   #65310

   Purpose

   Luminal ER hook only (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   STIM1 mutated for binding motif to microtubule (STIM1 Human)
   

   Use

   Tags

   streptavidin

   Expression

   Mammalian

   Mutation

   2 amino acids of STIM1 (IP from SxIP motif) were …

   Promoter

   CMV

   Available since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. TVBB N-term-mNeongreen

   Plasmid

   #169226

   Purpose

   Targeting vector backbone to support a knock-in of mNeongreen-Linker at the N-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked mNeongreen
   

   Use

   Targeting vector backbone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pINTO-NFH::mEzh2

   Plasmid

   #65925

   Purpose

   Expression of mouse Ezh2 fused to Flag-HA tag (N-terminus)

   Depositor

   Roberto Bonasio

   Article

   Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

   Insert

   Enhancer of zeste homolog 2 (Ezh2 Mouse)
   

   Use

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TO (T-REx) and CMV/TetO2 (T-REx)

   Available since

   July 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCI-Myc3-HPS4

   Plasmid

   #133931

   Purpose

   Expresses 3xMyc-HPS4 construct in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Martina et al J Biol Chem. 2003 Aug 1;278(31):29376-84

   Insert

   HPS4 (HPS4 Human)
   

   Use

   Tags

   3xMyc tag

   Expression

   Mammalian

   Mutation

   Glutamic acid 229 to Glycine; Valine 552 to Methi…

   Promoter

   CMV I.E.

   Available since

   Nov. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCh-BICD2*-strep

   Plasmid

   #120168

   Purpose

   Expresses a chimera of mCherry (fluorescent tag), a BICD2-based adaptor and streptavidin

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   BICD cargo adaptor 2 (Bicd2 Mouse)
   

   Use

   Tags

   Streptavidin and mCherry

   Expression

   Mammalian

   Mutation

   Truncated BICD2: 15-595 aa

   Promoter

   CMV

   Available since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pINTO-NSA::mEzh2

    Plasmid

    #61725

    Purpose

    Expression of mouse Ezh2 fused to monomeric streptavidin (N-terminus)

    Depositor

    Roberto Bonasio

    Article

    Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

    Insert

    Enhancer of zeste homolog 2 (Ezh2 Mouse)
    

    Use

    Tags

    mSA (monomeric streptavidin)

    Expression

    Mammalian

    Mutation

    Promoter

    T-REx (CMV + 2xTetO2)

    Available since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. 2xmyc-RAB35

    Plasmid

    #164632

    Purpose

    Expresses RAB35 in mammalian cells with a 2xmyc tag

    Depositor

    Mark Cookson

    Article

    Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

    Insert

    RAB35 (RAB35 Human)
    

    Use

    Tags

    2xmyc

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC19-U6-BsmBI_cassette-CjeCas9-sgRNA (KAC482)

    Plasmid

    #133793

    Purpose

    U6 promoter sgRNA entry vector used for all CjeCas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V2-TGAA linker sgRNA architecture from Kim et al. Nature Communications 2017

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    CjeCas9 sgRNA entry vector
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V2-TGAA linker sgRNA architecture from Kim et al.…

    Promoter

    U6

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. TVBB C-term-Dendra2

    Plasmid

    #169216

    Purpose

    Targeting vector backbone to support a knock-in of Linker-Dendra2 at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked Dendra2-P2A-Puro
    

    Use

    Targeting vector backbone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. PX-TIR-GFP

    Plasmid

    #52737

    Purpose

    Retroviral vector expresses the lipid binding domain of p40-phox fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable marker

    Depositor

    Jonathan Kagan

    Article

    Bonham et al Cell. 2014 Feb 13;156(4):705-16. doi: 10

    Insert

    Toll/IL-1 Receptor adaptor protein (TIRAP Human)
    

    Use

    Retroviral

    Tags

    GFP and p40 Phox lipid binding domain

    Expression

    Mammalian

    Mutation

    Deleted amino acids 1-85

    Promoter

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pENTR-AtmiR173aTS-B/c

    Plasmid

    #227964

    Purpose

    Entry plasmid with AtmiR173aTS and a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

    Depositor

    Alberto Carbonell

    Article

    Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Inserts

    B/c
    AtmiR173aTS
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMDC32B-AtmiR173aTS-B/c

    Plasmid

    #227965

    Purpose

    Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. For expressing syn-tasiRNAs in Arabidopsis thaliana.

    Depositor

    Alberto Carbonell

    Article

    Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Inserts

    B/c
    AtmiR173aTS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S and None

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR-NbmiR482aTS-B/c

    Plasmid

    #227966

    Purpose

    Entry plasmid with NbmiR482aTS and a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

    Depositor

    Alberto Carbonell

    Article

    Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Inserts

    B/c
    NbmiR482aTS
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMDC32B-NbmiR482aTS-B/c

    Plasmid

    #227967

    Purpose

    Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. For expressing syn-tasiRNAs in Nicotiana benthamiana.

    Depositor

    Alberto Carbonell

    Article

    Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Inserts

    B/c
    NbmiR482aTS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S and None

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Str-KDEL_SBP-EGFP-CCR5-Cys3A

    Plasmid

    #222313

    Purpose

    Synchronize the trafficking of CCR5-Cys3A from the ER.

    Depositor

    Franck Perez

    Article

    Boncompain et al Sci Adv. 2019 Oct 16;5(10):eaax0821. doi

    Insert

    Streptavidin-KDEL and CCR5-Cys3A fused to SBP-EGFP (CCR5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C321A, C323A, C324A

    Promoter

    CMV

    Available since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOTTC1751 - pcDNA3.1 CMV-IE V5-ER-LBD

    Plasmid

    #201818

    Purpose

    A plasmid expressing the ligand-binding domain of estrogen receptor with a V5 epitope tag from a CMV promoter

    Depositor

    Christopher Richie

    Article

    Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

    Insert

    V5-ER-LBD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-IE

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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