We narrowed to 32,058 results for: eng

1. CRISPRoff-mScarletI

   Plasmid

   #217783

   Purpose

   Expresses CRISPRoff-mScarletI (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-mScarletI-KRAB) downstream of the CAG promoter for gene epigenetic silencing

   Depositor

   Adriano Aguzzi

   Article

   Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

   Insert

   CRISPRoff-mScarletI (DNMT3A-DNMT3L-dCas9-mScarletI-KRAB)
   

   Tags

   2xNLS, DNMT3A-DNMT3L, HA, KRAB, and mScarletI

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-pLenti-ACTA2-R179 (WT) (LLH661)

   Plasmid

   #242682

   Purpose

   Plasmid for expression of ACTA2-R179 (wild-type) cDNA from a lentiviral cassette

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

   Insert

   pLenti-ACTA2-R179_cDNA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. XE92 XGCNF-EnR

   Plasmid

   #17029

   Depositor

   Randall Moon

   Article

   Song et al Dev Biol. 1999 Sep 1;213(1):170-9. doi:

   Insert

   XGCNF-EnR (en Fly)
   

   Use

   Xenopus expression

   Available Since

   March 14, 2008

   Availability

   Academic Institutions and Nonprofits only

4. hNTo1-qgRNA-pYJA5

   Plasmid

   #217781

   Purpose

   Non-targeting control 1 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning

   Depositor

   Adriano Aguzzi

   Article

   Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

   Insert

   Quadruple sgRNA-expression cassette for nontargeting control for gene ablation
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Expression

   Mammalian

   Promoter

   human U6, mouse U6, human H1, human 7SK

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. hNTa1-qgRNA-pYJA5

   Plasmid

   #217779

   Purpose

   Non-targeting control 1 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning

   Depositor

   Adriano Aguzzi

   Article

   Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

   Insert

   Quadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Expression

   Mammalian

   Promoter

   human U6, mouse U6, human H1, human 7SK

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-ABE8.8m-WT-P2A-EGFP (BKS953)

   Plasmid

   #242651

   Purpose

   CMV promoter expression plasmid for human codon optimized ABE8.8m A-to-G base editor with SpCas9(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

   Insert

   pCMV-ABE8.8m-SpCas9-P2A-EGFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   ABE8.8 mutations in TadA

   Promoter

   CMV and T7

   Available Since

   Oct. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. hNTo2-qgRNA-pYJA5

   Plasmid

   #217782

   Purpose

   Non-targeting control 2 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning

   Depositor

   Adriano Aguzzi

   Article

   Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

   Insert

   Quadruple sgRNA-expression cassette for nontargeting control for gene ablation
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Expression

   Mammalian

   Promoter

   human U6, mouse U6, human H1, human 7SK

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. hNTa2-qgRNA-pYJA5

   Plasmid

   #217780

   Purpose

   Non-targeting control 2 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning

   Depositor

   Adriano Aguzzi

   Article

   Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

   Insert

   Quadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Expression

   Mammalian

   Promoter

   human U6, mouse U6, human H1, human 7SK

   Available Since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRhaB-8xHis-ABE8e-eVRQR (LLH551)

   Plasmid

   #242658

   Purpose

   pRhaB promoter expression plasmid for ABE8e-eVRQR with an N-terminal His8-tag

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

   Insert

   pRhaB-8xHis-BPNLS-TadA8e-SpCas9(D10A)-eVRQR-BPNLS
   

   Use

   CRISPR

   Tags

   8x-His

   Expression

   Bacterial

   Mutation

   eVRQR mutations in SpCas9(S55R/D1135V/G1218R/R133…

   Promoter

   RhaB

   Available Since

   Nov. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-eVRQR-P2A-EGFP (KAC1028)

    Plasmid

    #242663

    Purpose

    CMV promoter expression plasmid for human codon optimized SpCas9-eVRQR(S55R)-BPNLS(SV40)-3xFLAG-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

    Insert

    pCMV-SpCas9-eVRQR(S55R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    eVRQR mutations in SpCas9(S55R/D1135V/G1218R/R133…

    Promoter

    CMV and T7

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-SpCas9-VQR-P2A-EGFP (LRF199)

    Plasmid

    #242664

    Purpose

    CMV promoter expression plasmid for human codon optimized SpCas9-VQR-BPNLS(SV40)-3xFLAG-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

    Insert

    pCMV-SpCas9-VQR-BPNLS(SV40)-3xFLAG-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    VQR mutations in SpCas9(D1135V/R1335Q/T1337R)

    Promoter

    CMV and T7

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-PE2-SpG (HES824)

    Plasmid

    #242689

    Purpose

    Prime editing in mammalian cells with SpG-based PE2 prime editors

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

    Insert

    pCMV-NLS-SpCas9(H840A)-M-MLV-RT(D200N,T306K,W313F,T330P,L603W)-NLS
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    PE2 mutations in MMLV RT, SpG mutations in SpCas9…

    Promoter

    CMV

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. CAG-EnR

    Plasmid

    #19715

    Depositor

    Connie Cepko

    Article

    Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

    Insert

    engrailed (en Fly)
    

    Expression

    Mammalian

    Mutation

    Drosophila engrailed repressor domain

    Available Since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pCMV-ABE8e-eVRQR-P2A-EGFP (HES1425)

    Plasmid

    #242657

    Purpose

    CMV promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with neVRQR(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

    Insert

    pCMV-ABE8e-SpCas9-VRQR(S55R)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA, eVRQR mutations in SpCas…

    Promoter

    CMV and T7

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-ABE8.8m-SpG-P2A-EGFP (BKS965)

    Plasmid

    #242652

    Purpose

    CMV promoter expression plasmid for human codon optimized ABE8.8m A-to-G base editor with SpG(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

    Insert

    pCMV-ABE8.8m-SpG-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8.8 mutations in TadA, SpG mutations in SpCas9…

    Promoter

    CMV and T7

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-ABE8.20m-eVRQR-P2A-EGFP (LLH569)

    Plasmid

    #242656

    Purpose

    CMV promoter expression plasmid for human codon optimized ABE8.20 A-to-G base editor with neVRQR(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

    Insert

    pCMV-ABE8.20m-VRQR-S55R-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8.20 mutations in TadA, eVRQR mutations in SpC…

    Promoter

    CMV and T7

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. C-term AAV-SpCas9-VRQR-gRNA_entry (HES1477)

    Plasmid

    #242660

    Purpose

    CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and pU6 SpCas9 gRNA entry cassette

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

    Insert

    pAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-SpCas9_gRNA_entrycassette
    

    Use

    AAV and CRISPR

    Tags

    BPNLS

    Mutation

    VRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…

    Promoter

    CBh

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. C-term AAV-eVRQR-ACTA2-gRNA-A4 (mouse) (LLH310)

    Plasmid

    #242662

    Purpose

    CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and mouse gRNA-A4

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

    Insert

    pAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-ACTA2_mouse_NGA_gRNA-pU6
    

    Use

    AAV and CRISPR

    Tags

    BPNLS

    Mutation

    VRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…

    Promoter

    CBh

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. C-term AAV-eVRQR-ACTA2-gRNA-A4 (human) (LLH301)

    Plasmid

    #242661

    Purpose

    CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and human gRNA-A4

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

    Insert

    pAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-ACTA2_human_NGA_gRNA-pU6
    

    Use

    AAV and CRISPR

    Tags

    BPNLS

    Mutation

    VRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…

    Promoter

    CBh

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-SpCas9-VRQR(+R221K)-P2A-EGFP (LRF95)

    Plasmid

    #242666

    Purpose

    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(R221K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

    Insert

    pCMV-SpCas9-VRQR(R221K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    VRQR + R221K mutations in SpCas9(R221K/D1135V/G12…

    Promoter

    CMV and T7

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge