We narrowed to 5,817 results for: pCas

1. pFREE2-SpCas9-sgRNA

   Plasmid

   #179582

   Purpose

   Expresses spCas9 and gRNA that targets ColE1 plasmids for curing

   Depositor

   Kevin Solomon

   Article

   Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

   Insert

   Cas9
   

   Expression

   Bacterial

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-Venus-MDGA1

   Plasmid

   #82543

   Purpose

   Overexpression vector for Venus-MDGA1 in neurons

   Depositor

   Alice Ting

   Article

   Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

   Insert

   MDGA1 (MDGA1 Human)
   

   Tags

   Venus fluorescent protein

   Expression

   Mammalian

   Promoter

   chicken beta actin

   Available Since

   Sept. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-Ace2_D92N_E199V-mRuby3

   Plasmid

   #129257

   Purpose

   Express Ace2_D92N_E199V-mRuby3 in mammalian cells

   Depositor

   Eric Schreiter

   Article

   Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

   Insert

   Ace2_D92N_E199V-mRuby3
   

   Expression

   Mammalian

   Mutation

   D92N, E199V

   Promoter

   CAG

   Available Since

   Aug. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. DN2-Gli3 in AINGpCAGGS

   Plasmid

   #121969

   Purpose

   strong Gli3 transcriptional activator

   Depositor

   James Briscoe

   Article

   Stamataki et al Genes Dev. 2005 Mar 1;19(5):626-41. doi:

   Insert

   DN2Gli3 (GLI3 Human)
   

   Use

   Chick electroporation

   Tags

   5xmyc

   Expression

   Mammalian

   Mutation

   N terminus deletion (nt1-1401)

   Available Since

   April 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAFNF-ECFP-Actb

   Plasmid

   #169787

   Purpose

   An actin FRET sensor. Must be expressed with Venus-actin for FRET.

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Actb (Actb Mouse)
   

   Tags

   ECFP

   Expression

   Mammalian

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-GBP7-p65AD

   Plasmid

   #49441

   Purpose

   Expression of a GFP-dependent transcription factor component in mammalian cells

   Depositor

   Connie Cepko

   Article

   Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

   Insert

   GBP7-p65 transactivation domain fusion protein
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-C1V1-TT-mRuby2-ST

   Plasmid

   #109124

   Purpose

   Cation channelrhodopsin C1V1 fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

   Depositor

   Hillel Adesnik

   Article

   Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

   Insert

   C1V1-TT-mRuby2-ST
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9_BB_2A-GFP_MAPRE1-gRNA#1

   Plasmid

   #107726

   Purpose

   Knock out of EB1 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTrex-b-NLS-hSpCas9

   Plasmid

   #62543

   Purpose

   Expression of Cas9 in T. cruzi

   Depositor

   Rick Tarleton

   Article

   Peng et al MBio. 2014 Dec 30;6(1). pii: e02097-14.

   Insert

   NLS-hSpCas9
   

   Use

   CRISPR; Trypanosoma cruzi expression

   Tags

   3xFLAG and NLS

   Promoter

   Ribo-HX1

   Available Since

   April 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. G1333 DddAtox-N–dSpCas9

    Plasmid

    #157833

    Purpose

    expresses split DddAtox-Cas9 construct in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    bpNLS–G1333 DddAtox-N–dSpCas9–UGI–UGI–bpNLS
    

    Expression

    Mammalian

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-cofilin S3A

    Plasmid

    #163604

    Purpose

    Expresses constitutively active cofilin in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    cofilin1 (Cfl1 Mouse)
    

    Expression

    Mammalian

    Mutation

    S3A substitution to make cofilin constitutively a…

    Available Since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMA-SpCas9-g10

    Plasmid

    #80793

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 10/20/30 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG1.1-TSSK2/3xFLAG

    Plasmid

    #199625

    Purpose

    Expression vector of mouse TSSK2. 3xFLAG tag at C-terminus.

    Depositor

    Masahito Ikawa

    Article

    Shimada et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

    Insert

    Tssk2 mRNA (Tssk2 Mouse)
    

    Tags

    3xFLAG

    Promoter

    CAG

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-Ace2_D92N_E199V-mNeon-ST

    Plasmid

    #129261

    Purpose

    Express soma-localized Ace2_D92N_E199V-mNeon in mammalian cells

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

    Insert

    Ace2_D92N_E199V-mNeon-ST
    

    Expression

    Mammalian

    Mutation

    D92N, E199V

    Promoter

    CAG

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCaST-elav-VP16AD

    Plasmid

    #15308

    Depositor

    Benjamin White

    Article

    Luan et al Neuron. 2006 Nov 9. 52(3):425-36.

    Insert

    VP16AD-ZIP+
    

    Expression

    Insect

    Available Since

    July 6, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-Venus-MDGA2

    Plasmid

    #82544

    Purpose

    Overexpression vector for Venus-MDGA2 in neurons

    Depositor

    Alice Ting

    Article

    Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

    Insert

    MDGA2 (Mdga2 Rat)
    

    Tags

    Venus fluorescent protein

    Expression

    Mammalian

    Promoter

    chicken beta actin

    Available Since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330-NL-DHFR-SpCas9

    Plasmid

    #124522

    Purpose

    Expresses SpCas9 fused to DHFR domains on both the N-terminus and an internal loop in mammalian cells

    Depositor

    Amit Choudhary

    Article

    Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

    Insert

    NL-DHFR-SpCas9
    

    Use

    AAV

    Tags

    Destabilized domain of E.coli dihydrofolate reduc…

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-mDrc7-FLAG

    Plasmid

    #176470

    Purpose

    Expression vector of mouse dynein regulatory complex subunit 7 (Drc7) tagged with FLAG at C-terminus.

    Depositor

    Masahito Ikawa

    Article

    Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

    Insert

    dynein regulatory complex subunit 7 (Drc7 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330_Actb sgRNA / hSpCas9

    Plasmid

    #172832

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1of Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 1 of Actb under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-ChETA-mRuby2-ST

    Plasmid

    #109126

    Purpose

    Cation channelrhodopsin ChETA fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    ChETA-mRuby2-ST
    

    Expression

    Mammalian

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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